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Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

Li R, Jiang Y, Wang X, Yang J, Gao Y, Zi X, Zhang X, Gao H, Hu N - Springerplus (2013)

Bottom Line: Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii.P. mandelii CBS-1 grew optimally at 20°C.When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB.

View Article: PubMed Central - PubMed

Affiliation: Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.

ABSTRACT
A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

No MeSH data available.


Related in: MedlinePlus

Effect of culture conditions onP. mandeliiCBS-1 growth. Effects of temperature (A), C/N ratio (B), pH (C), and NaCl concentration (D) were investigated.
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Fig3: Effect of culture conditions onP. mandeliiCBS-1 growth. Effects of temperature (A), C/N ratio (B), pH (C), and NaCl concentration (D) were investigated.

Mentions: Sucrose and monosodium glutamate were the optimum carbon and nitrogen sources for culturing CBS-1 (Table 2) at a C/N ratio of 5 (Figure 3B). Strain CBS-1 grew to significant numbers between 10 and 35°C, and the optimum growth temperature was 20°C (Figure 3A). Strain CBS-1 grew between pH 6.0 and 9.0 and optimally between pH 7.0 and 7.5 (Figure 3C). Significant growth was observed in media containing 0–3% NaCl (Figure 3D).Table 2


Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

Li R, Jiang Y, Wang X, Yang J, Gao Y, Zi X, Zhang X, Gao H, Hu N - Springerplus (2013)

Effect of culture conditions onP. mandeliiCBS-1 growth. Effects of temperature (A), C/N ratio (B), pH (C), and NaCl concentration (D) were investigated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755782&req=5

Fig3: Effect of culture conditions onP. mandeliiCBS-1 growth. Effects of temperature (A), C/N ratio (B), pH (C), and NaCl concentration (D) were investigated.
Mentions: Sucrose and monosodium glutamate were the optimum carbon and nitrogen sources for culturing CBS-1 (Table 2) at a C/N ratio of 5 (Figure 3B). Strain CBS-1 grew to significant numbers between 10 and 35°C, and the optimum growth temperature was 20°C (Figure 3A). Strain CBS-1 grew between pH 6.0 and 9.0 and optimally between pH 7.0 and 7.5 (Figure 3C). Significant growth was observed in media containing 0–3% NaCl (Figure 3D).Table 2

Bottom Line: Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii.P. mandelii CBS-1 grew optimally at 20°C.When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB.

View Article: PubMed Central - PubMed

Affiliation: Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.

ABSTRACT
A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

No MeSH data available.


Related in: MedlinePlus