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Analysis of the Chemical Constituents of Agaricus brasiliensis.

Cho SM, Jang KY, Park HJ, Park JS - Mycobiology (2008)

Bottom Line: Through GP-HPLC, the substance was found to be homogeneous.By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue.In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agricultural Science and Technology, R.D.A. Suwon 441-707, Korea.

ABSTRACT
This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by (13)C-NMR. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue. The β-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

No MeSH data available.


Immuno-stimulating activity on antibody forming ability of B-lymphocytes of the polysaccharide purified from hot-water extract of A. blasiliensis fruiting body by the hemolytic suspension assay.
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Figure 2: Immuno-stimulating activity on antibody forming ability of B-lymphocytes of the polysaccharide purified from hot-water extract of A. blasiliensis fruiting body by the hemolytic suspension assay.

Mentions: To find out its effect on the antibody production of B-lymphocytes, this study directly added the polysaccharide to the culture medium at concentrations of 1, 10, and 100 µg/ml and LPS (6.25 µg/ml) was used as the positive control. The polysaccharide increased the antibody production of B-lymphocytes (Fig. 2).


Analysis of the Chemical Constituents of Agaricus brasiliensis.

Cho SM, Jang KY, Park HJ, Park JS - Mycobiology (2008)

Immuno-stimulating activity on antibody forming ability of B-lymphocytes of the polysaccharide purified from hot-water extract of A. blasiliensis fruiting body by the hemolytic suspension assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755252&req=5

Figure 2: Immuno-stimulating activity on antibody forming ability of B-lymphocytes of the polysaccharide purified from hot-water extract of A. blasiliensis fruiting body by the hemolytic suspension assay.
Mentions: To find out its effect on the antibody production of B-lymphocytes, this study directly added the polysaccharide to the culture medium at concentrations of 1, 10, and 100 µg/ml and LPS (6.25 µg/ml) was used as the positive control. The polysaccharide increased the antibody production of B-lymphocytes (Fig. 2).

Bottom Line: Through GP-HPLC, the substance was found to be homogeneous.By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue.In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agricultural Science and Technology, R.D.A. Suwon 441-707, Korea.

ABSTRACT
This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by (13)C-NMR. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue. The β-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

No MeSH data available.