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Analysis of the Chemical Constituents of Agaricus brasiliensis.

Cho SM, Jang KY, Park HJ, Park JS - Mycobiology (2008)

Bottom Line: Through GP-HPLC, the substance was found to be homogeneous.By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue.In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agricultural Science and Technology, R.D.A. Suwon 441-707, Korea.

ABSTRACT
This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by (13)C-NMR. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue. The β-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

No MeSH data available.


13C-NMR spectrum at 125MHz and a supposed structure of the polysaccharide isolated from the hot water extract of A. blasiliensis fruiting body at 25℃ in D2O.
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Figure 1: 13C-NMR spectrum at 125MHz and a supposed structure of the polysaccharide isolated from the hot water extract of A. blasiliensis fruiting body at 25℃ in D2O.

Mentions: The 13C NMR spectrum of the polysaccharide (AG-6, Cho et al., 1999) isolated from the hot water extract of A. blasiliensis fruiting body (Fig. 1) provides useful information on its composition and sequence. The structure was in accordance with the values reported in previous literature (Schulz and Rapp, 1991; Gutierrez et al., 1996; Dong et al., 2002). Two closely located signals at 105.8 ppm (parts per million) and 105.2 ppm in the anomeric region indicated that all the anomeric carbons adopted the β configuration. The substituted C-6 signal could be identified at 71.7 ppm and the non-substituted C-6 signal at 63.5 ppm. The signals at 83.6 ppm and 78.7 ppm were assigned to the substituted C-3 and the non-substituted C-3, respectively. The other signals at 78.4 ppm, 77.7 ppm, 75.9 ppm, and 72.3 ppm suggested substituted adjacent glucose residues, non-substituted adjacent glucose residues with a branched β-glucopyranosyl, and two other non-branched β-glucopyranosyl residues, respectively. These results indicated β-(1→3)-(1→6)-glucan composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching to a (1→6)-linked D-glucopyranosyl residue.


Analysis of the Chemical Constituents of Agaricus brasiliensis.

Cho SM, Jang KY, Park HJ, Park JS - Mycobiology (2008)

13C-NMR spectrum at 125MHz and a supposed structure of the polysaccharide isolated from the hot water extract of A. blasiliensis fruiting body at 25℃ in D2O.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755252&req=5

Figure 1: 13C-NMR spectrum at 125MHz and a supposed structure of the polysaccharide isolated from the hot water extract of A. blasiliensis fruiting body at 25℃ in D2O.
Mentions: The 13C NMR spectrum of the polysaccharide (AG-6, Cho et al., 1999) isolated from the hot water extract of A. blasiliensis fruiting body (Fig. 1) provides useful information on its composition and sequence. The structure was in accordance with the values reported in previous literature (Schulz and Rapp, 1991; Gutierrez et al., 1996; Dong et al., 2002). Two closely located signals at 105.8 ppm (parts per million) and 105.2 ppm in the anomeric region indicated that all the anomeric carbons adopted the β configuration. The substituted C-6 signal could be identified at 71.7 ppm and the non-substituted C-6 signal at 63.5 ppm. The signals at 83.6 ppm and 78.7 ppm were assigned to the substituted C-3 and the non-substituted C-3, respectively. The other signals at 78.4 ppm, 77.7 ppm, 75.9 ppm, and 72.3 ppm suggested substituted adjacent glucose residues, non-substituted adjacent glucose residues with a branched β-glucopyranosyl, and two other non-branched β-glucopyranosyl residues, respectively. These results indicated β-(1→3)-(1→6)-glucan composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching to a (1→6)-linked D-glucopyranosyl residue.

Bottom Line: Through GP-HPLC, the substance was found to be homogeneous.By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue.In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agricultural Science and Technology, R.D.A. Suwon 441-707, Korea.

ABSTRACT
This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by (13)C-NMR. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By (13)C-NMR analysis, the immuno-stimulating substance was identified as β-(1→3) (1→6)-glucan, composed of a backbone with (1→3)-linked D-glucopyranosyl residues branching a (1→6)-linked D-glucopyranosyl residue. The β-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

No MeSH data available.