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Effect of Casing Layer on Growth Promotion of the Edible Mushroom Pleurotus ostreatus.

Cho YS, Weon HY, Joh JH, Lim JH, Kim KY, Son ES, Lee CS, Cho BG - Mycobiology (2008)

Bottom Line: Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil.Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil.The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biochemistry, Konkuk University, Chungju 380-701, Korea.

ABSTRACT
Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil. Fluorescent Pseudomonas was predominant in the non-sterilized casing layer soil. Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil. In particular, an increase in the fluorescent Pseudomonas population was observed in the non-sterilized casing layer accompanied by induction of fruiting body and enhanced mushroom production yield. The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

No MeSH data available.


Related in: MedlinePlus

Casing layer treatment process and growth of P. ostreatus. A: Completed nutritional growth of mycelium. B: Casing layer on mycelium. C: Initiated primodia formation on the casing layer. D: Promoted fruiting body.
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Figure 2: Casing layer treatment process and growth of P. ostreatus. A: Completed nutritional growth of mycelium. B: Casing layer on mycelium. C: Initiated primodia formation on the casing layer. D: Promoted fruiting body.

Mentions: P. ostreatus was cultured on potato dextrose agar (PDA). Bacteria from the casing layer of P. ostreatus were cultured on 10% TSA and P1 medium (Cho et al., 2003). Actinomycetes were grown on humic acid vitamin (HV) agar, and fungi were grown on Rose-Bengal agar. For identification of bacteria by fatty acid analysis, bacteria isolated from the casing layer soil of P. ostreatus were grown on TSA. Bacteria isolated from the casing layer soils were identified by fatty acid methyl ester (FAME) analysis following cultivation of the individual isolates on TSBA for 24 h at 28℃ prior to the analysis. FAME(fatty acid methyl ester) was conducted using the Sherlock Microbial Identification System (MIDI, Inc., Newark, DE, USA), using protocols recommended by the manufacturer. Bacteria were collected from the casing layer soil (sterilized and non-sterilized) after P. ostreatus was harvested (Fig. 2). The casing layer soil for this experiment was chosen according to the highest yield from 6 boxes of planted mushrooms which divided into sterilized soil (S-S), non-sterilized (N-S) soil and bulk soil.


Effect of Casing Layer on Growth Promotion of the Edible Mushroom Pleurotus ostreatus.

Cho YS, Weon HY, Joh JH, Lim JH, Kim KY, Son ES, Lee CS, Cho BG - Mycobiology (2008)

Casing layer treatment process and growth of P. ostreatus. A: Completed nutritional growth of mycelium. B: Casing layer on mycelium. C: Initiated primodia formation on the casing layer. D: Promoted fruiting body.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755250&req=5

Figure 2: Casing layer treatment process and growth of P. ostreatus. A: Completed nutritional growth of mycelium. B: Casing layer on mycelium. C: Initiated primodia formation on the casing layer. D: Promoted fruiting body.
Mentions: P. ostreatus was cultured on potato dextrose agar (PDA). Bacteria from the casing layer of P. ostreatus were cultured on 10% TSA and P1 medium (Cho et al., 2003). Actinomycetes were grown on humic acid vitamin (HV) agar, and fungi were grown on Rose-Bengal agar. For identification of bacteria by fatty acid analysis, bacteria isolated from the casing layer soil of P. ostreatus were grown on TSA. Bacteria isolated from the casing layer soils were identified by fatty acid methyl ester (FAME) analysis following cultivation of the individual isolates on TSBA for 24 h at 28℃ prior to the analysis. FAME(fatty acid methyl ester) was conducted using the Sherlock Microbial Identification System (MIDI, Inc., Newark, DE, USA), using protocols recommended by the manufacturer. Bacteria were collected from the casing layer soil (sterilized and non-sterilized) after P. ostreatus was harvested (Fig. 2). The casing layer soil for this experiment was chosen according to the highest yield from 6 boxes of planted mushrooms which divided into sterilized soil (S-S), non-sterilized (N-S) soil and bulk soil.

Bottom Line: Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil.Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil.The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biochemistry, Konkuk University, Chungju 380-701, Korea.

ABSTRACT
Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil. Fluorescent Pseudomonas was predominant in the non-sterilized casing layer soil. Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil. In particular, an increase in the fluorescent Pseudomonas population was observed in the non-sterilized casing layer accompanied by induction of fruiting body and enhanced mushroom production yield. The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

No MeSH data available.


Related in: MedlinePlus