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Ecological and physiological studies on soil fungi at Western region, libya.

El-Said AH, Saleem A - Mycobiology (2008)

Bottom Line: The most prevalent species from the three types of soils on the three types of media were Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Emericella nidulans, Fusarium oxysporum, Mycosphaerella tassiana, Nectria haematococca and Penicillium chrysogenum.Chaetomium globosum was in the top of fungi in producing endo-β-1,4-glucanases among the 42 tested isolates obtained from soils on cellulose-Czapek's agar.Maximum production of this enzyme by C. globosum obtained after 6 days of incubation at 30℃ with culture medium containing maltose as a carbon source and ammonium nitrate as a nitrogen source and pH initially adjusted to 6.

View Article: PubMed Central - PubMed

Affiliation: Botany Department, Faculty of Science, South Valley University, Qena, Egypt.

ABSTRACT
Sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. Cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-Czapek's agar at 28℃. The most common genera were Alternaria, Aspergillus, Emericella, Fusarium, Mycosphaerella, Nectria and Penicillium. The most prevalent species from the three types of soils on the three types of media were Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Emericella nidulans, Fusarium oxysporum, Mycosphaerella tassiana, Nectria haematococca and Penicillium chrysogenum. Chaetomium globosum was in the top of fungi in producing endo-β-1,4-glucanases among the 42 tested isolates obtained from soils on cellulose-Czapek's agar. Maximum production of this enzyme by C. globosum obtained after 6 days of incubation at 30℃ with culture medium containing maltose as a carbon source and ammonium nitrate as a nitrogen source and pH initially adjusted to 6.

No MeSH data available.


Cellulase production by C. globosum in culture media containing different carbon (A) and nitrogen (B) sources.
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Figure 3: Cellulase production by C. globosum in culture media containing different carbon (A) and nitrogen (B) sources.

Mentions: C. globosum was in the top of fungi in producing of endo 1,4-β-glucanase (Cx enzyme) in this investigation. Maximum production of the enzyme by C. globosum was achieved 6 days after incubation at 30℃ with the incorporation of maltose as carbon sucrce and NH4NO3 as nitrogen source in the culture medium which is initially adjusted to pH 6 (Figs. 2 and 3). These findings are almost in agreement with those reported by Sandhu and Kalra (1985) and Kalra and Sandhu (1986). They noticed that the maximum production of C1 and Cx enzymes produced by Trichoderma longibrachiatum and T. harzianum was achieved after 5~7 days of incubation at 27℃ but with the incorporation of 1% lactose in culture medium which initially adjusted to pH 5. They also found that CMC and malt extract were favourable for the enzyme production. The maximum production of exo- and endo-β-1,4-glucanase by C. globosum and Trichoderma viride were after 6 and 8 days of incubation at 25℃ with culture medium containing wheat bran as a carbon source and peptone as nitrogen source and initially adjusted to pH 6 (Abdel-Hafez et al., 1995; El-Said, 2001). Recently, El-Said et al. (2006) found that maximum production of endo-β-1,4-glucanase by F. oxysporum could be achieved after 8 days of incubation at 30℃ with the incoporation of carboxymethylcellulose as a carbon source and peptone as nitrogen source in the culture medium which initially adjusted to pH 6.


Ecological and physiological studies on soil fungi at Western region, libya.

El-Said AH, Saleem A - Mycobiology (2008)

Cellulase production by C. globosum in culture media containing different carbon (A) and nitrogen (B) sources.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755243&req=5

Figure 3: Cellulase production by C. globosum in culture media containing different carbon (A) and nitrogen (B) sources.
Mentions: C. globosum was in the top of fungi in producing of endo 1,4-β-glucanase (Cx enzyme) in this investigation. Maximum production of the enzyme by C. globosum was achieved 6 days after incubation at 30℃ with the incorporation of maltose as carbon sucrce and NH4NO3 as nitrogen source in the culture medium which is initially adjusted to pH 6 (Figs. 2 and 3). These findings are almost in agreement with those reported by Sandhu and Kalra (1985) and Kalra and Sandhu (1986). They noticed that the maximum production of C1 and Cx enzymes produced by Trichoderma longibrachiatum and T. harzianum was achieved after 5~7 days of incubation at 27℃ but with the incorporation of 1% lactose in culture medium which initially adjusted to pH 5. They also found that CMC and malt extract were favourable for the enzyme production. The maximum production of exo- and endo-β-1,4-glucanase by C. globosum and Trichoderma viride were after 6 and 8 days of incubation at 25℃ with culture medium containing wheat bran as a carbon source and peptone as nitrogen source and initially adjusted to pH 6 (Abdel-Hafez et al., 1995; El-Said, 2001). Recently, El-Said et al. (2006) found that maximum production of endo-β-1,4-glucanase by F. oxysporum could be achieved after 8 days of incubation at 30℃ with the incoporation of carboxymethylcellulose as a carbon source and peptone as nitrogen source in the culture medium which initially adjusted to pH 6.

Bottom Line: The most prevalent species from the three types of soils on the three types of media were Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Emericella nidulans, Fusarium oxysporum, Mycosphaerella tassiana, Nectria haematococca and Penicillium chrysogenum.Chaetomium globosum was in the top of fungi in producing endo-β-1,4-glucanases among the 42 tested isolates obtained from soils on cellulose-Czapek's agar.Maximum production of this enzyme by C. globosum obtained after 6 days of incubation at 30℃ with culture medium containing maltose as a carbon source and ammonium nitrate as a nitrogen source and pH initially adjusted to 6.

View Article: PubMed Central - PubMed

Affiliation: Botany Department, Faculty of Science, South Valley University, Qena, Egypt.

ABSTRACT
Sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. Cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-Czapek's agar at 28℃. The most common genera were Alternaria, Aspergillus, Emericella, Fusarium, Mycosphaerella, Nectria and Penicillium. The most prevalent species from the three types of soils on the three types of media were Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Emericella nidulans, Fusarium oxysporum, Mycosphaerella tassiana, Nectria haematococca and Penicillium chrysogenum. Chaetomium globosum was in the top of fungi in producing endo-β-1,4-glucanases among the 42 tested isolates obtained from soils on cellulose-Czapek's agar. Maximum production of this enzyme by C. globosum obtained after 6 days of incubation at 30℃ with culture medium containing maltose as a carbon source and ammonium nitrate as a nitrogen source and pH initially adjusted to 6.

No MeSH data available.