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Antifungal Activity of Securinine against Some Plant Pathogenic Fungi.

Singh AK, Pandey MB, Singh S, Singh AK, Singh UP - Mycobiology (2008)

Bottom Line: The alkaloid securinine was assessed against spore germination of some plant pathogenic and saprophytic fungi (Alternaria alternata, Alternaria brassicae, Alternaria brassicicola, Curvularia lunata, Curvularia maculans, Curvularia pallenscens, Colletotrichum musae, Colletotrichum sp., Erysiphe pisi, Helminthosporium echinoclova, Helminthosporium spiciferum, Heterosporium sp.).Spore germinations of all the tested fungi were inhibited.Alternaria brassicicola, C. lunata, C. pallenscens and H. spiciferum were highly sensitive as complete inhibition of spore germination was observed at very low concentrations (200 ppm).

View Article: PubMed Central - PubMed

Affiliation: Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221005, India.

ABSTRACT
The alkaloid securinine was assessed against spore germination of some plant pathogenic and saprophytic fungi (Alternaria alternata, Alternaria brassicae, Alternaria brassicicola, Curvularia lunata, Curvularia maculans, Curvularia pallenscens, Colletotrichum musae, Colletotrichum sp., Erysiphe pisi, Helminthosporium echinoclova, Helminthosporium spiciferum, Heterosporium sp.). Spore germinations of all the tested fungi were inhibited. Alternaria brassicicola, C. lunata, C. pallenscens and H. spiciferum were highly sensitive as complete inhibition of spore germination was observed at very low concentrations (200 ppm).

No MeSH data available.


Structure of securinine and allosecurinine.
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Figure 1: Structure of securinine and allosecurinine.

Mentions: The whole plant of P. amarus was collected from Varanasi and dried in sun light. The dried, powdered whole plant (4 kg) was extracted with methanol in a Soxhlet extractor. The methanol extract was dried on water bath and extracted with 7% aqueous citric acid. The acidic fraction was basified with NH4OH and extracted with CHCl3. The CHCl3 fraction was concentrated and chromatographed over SiO2 gel column eluting with solvents of increasing polarity and each eluted fraction was monitored by thin layer chromatography for their homogeinity. Fractions eluted from CHCl3-methanol (1 : 2) were mixed together and crystallised from methanol which furnished an alkaloid (56 mg), as colourless granules, Rf 0.45 (CHCl3-methanol, 4 : 1), m.p. 140~43℃. It exhibited IR absorption bands at 1840 and 1760 cm-1 and UV absorption maxima at 256 nm. Its molecular formula was determined as C13H15NO2 from its molecular ion peak at m/z 217 [M+] in the mass spectrum. The spectral data, i.e.1H-NMR, 13C-NMR and MS were identical with reported data of securinine. Finally, it was identified as securinine (1) (Fig. 1) (Manske, 1977) by direct comparison with an authentic sample (mmp, co-TLC and superimposable IR).


Antifungal Activity of Securinine against Some Plant Pathogenic Fungi.

Singh AK, Pandey MB, Singh S, Singh AK, Singh UP - Mycobiology (2008)

Structure of securinine and allosecurinine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755242&req=5

Figure 1: Structure of securinine and allosecurinine.
Mentions: The whole plant of P. amarus was collected from Varanasi and dried in sun light. The dried, powdered whole plant (4 kg) was extracted with methanol in a Soxhlet extractor. The methanol extract was dried on water bath and extracted with 7% aqueous citric acid. The acidic fraction was basified with NH4OH and extracted with CHCl3. The CHCl3 fraction was concentrated and chromatographed over SiO2 gel column eluting with solvents of increasing polarity and each eluted fraction was monitored by thin layer chromatography for their homogeinity. Fractions eluted from CHCl3-methanol (1 : 2) were mixed together and crystallised from methanol which furnished an alkaloid (56 mg), as colourless granules, Rf 0.45 (CHCl3-methanol, 4 : 1), m.p. 140~43℃. It exhibited IR absorption bands at 1840 and 1760 cm-1 and UV absorption maxima at 256 nm. Its molecular formula was determined as C13H15NO2 from its molecular ion peak at m/z 217 [M+] in the mass spectrum. The spectral data, i.e.1H-NMR, 13C-NMR and MS were identical with reported data of securinine. Finally, it was identified as securinine (1) (Fig. 1) (Manske, 1977) by direct comparison with an authentic sample (mmp, co-TLC and superimposable IR).

Bottom Line: The alkaloid securinine was assessed against spore germination of some plant pathogenic and saprophytic fungi (Alternaria alternata, Alternaria brassicae, Alternaria brassicicola, Curvularia lunata, Curvularia maculans, Curvularia pallenscens, Colletotrichum musae, Colletotrichum sp., Erysiphe pisi, Helminthosporium echinoclova, Helminthosporium spiciferum, Heterosporium sp.).Spore germinations of all the tested fungi were inhibited.Alternaria brassicicola, C. lunata, C. pallenscens and H. spiciferum were highly sensitive as complete inhibition of spore germination was observed at very low concentrations (200 ppm).

View Article: PubMed Central - PubMed

Affiliation: Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221005, India.

ABSTRACT
The alkaloid securinine was assessed against spore germination of some plant pathogenic and saprophytic fungi (Alternaria alternata, Alternaria brassicae, Alternaria brassicicola, Curvularia lunata, Curvularia maculans, Curvularia pallenscens, Colletotrichum musae, Colletotrichum sp., Erysiphe pisi, Helminthosporium echinoclova, Helminthosporium spiciferum, Heterosporium sp.). Spore germinations of all the tested fungi were inhibited. Alternaria brassicicola, C. lunata, C. pallenscens and H. spiciferum were highly sensitive as complete inhibition of spore germination was observed at very low concentrations (200 ppm).

No MeSH data available.