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Identification of Microdochium bolleyi Associated with Basal Rot of Creeping Bent Grass in Korea.

Hong SK, Kim WG, Choi HW, Lee SY - Mycobiology (2008)

Bottom Line: Symptoms of basal rot occurred sporadically on creeping bent grasses growing at a golf course in Hampyeong, Korea in April 2007.Ten isolates of Microdochium sp. were obtained from leaves and crowns of the diseased bent grasses.All isolates were identified as Microdochium bolleyi based on morphological, cultural, and molecular characteristics.

View Article: PubMed Central - PubMed

Affiliation: Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.

ABSTRACT
Symptoms of basal rot occurred sporadically on creeping bent grasses growing at a golf course in Hampyeong, Korea in April 2007. Ten isolates of Microdochium sp. were obtained from leaves and crowns of the diseased bent grasses. All isolates were identified as Microdochium bolleyi based on morphological, cultural, and molecular characteristics. This is the first report on M. bolleyi associated with basal rot on creeping bent grass in Korea.

No MeSH data available.


Mycelial growth of Microdochium bolleyi isolates on PDA, MA, and OA after a week of incubation at different temperatures in darkness.
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Figure 3: Mycelial growth of Microdochium bolleyi isolates on PDA, MA, and OA after a week of incubation at different temperatures in darkness.

Mentions: Recently developed colonies on PDA were white, and dark-colored chylamydospores formed leading to discoloration of with mycelial growth being optimal at 23℃ (Fig. 3). Two distinct types of conidiogenous cells were evident: ampullate and cylindrical (Figs. 4A and 4B). They formed laterally along with hyaline hyphal cells. Both types were hyaline and thin-walled. The size of the types were 3.1~6.4 × 2.5~3.8 µm (average 4.9 × 3.2 µm) and 1.5~2.7 (~5.9) × 0.8~1.4 µm (average 2.1 × 1.0 µm), respectively. Chlamydospores that formed in chain or clusters in older cultures were brown, swollen, thick walled, and were 5.2~14.9 × 4.4~11.3 µm (average 8.9 × 8.3 µm) in size (Fig. 4C). Conidia formed either singly or in a cluster on each locus of the conidiogenous cells (Fig. 4D). The conidia were hyaline, smooth, one-celled, thin-walled, lunate, and 5.0~8.7 × 1.6~2.3 µm (average 6.4 × 1.9 µm) in size. All 10 isolates were identified as Microdochium bolleyi (Sprauge) de Hoog and Herm.-Nijh. based on their morphological and cultural characteristics (Table 1). These characteristics were consistent with previous observations (De Hoog and Hermanides-Nijhof, 1977; Von Arx, 1982).


Identification of Microdochium bolleyi Associated with Basal Rot of Creeping Bent Grass in Korea.

Hong SK, Kim WG, Choi HW, Lee SY - Mycobiology (2008)

Mycelial growth of Microdochium bolleyi isolates on PDA, MA, and OA after a week of incubation at different temperatures in darkness.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755238&req=5

Figure 3: Mycelial growth of Microdochium bolleyi isolates on PDA, MA, and OA after a week of incubation at different temperatures in darkness.
Mentions: Recently developed colonies on PDA were white, and dark-colored chylamydospores formed leading to discoloration of with mycelial growth being optimal at 23℃ (Fig. 3). Two distinct types of conidiogenous cells were evident: ampullate and cylindrical (Figs. 4A and 4B). They formed laterally along with hyaline hyphal cells. Both types were hyaline and thin-walled. The size of the types were 3.1~6.4 × 2.5~3.8 µm (average 4.9 × 3.2 µm) and 1.5~2.7 (~5.9) × 0.8~1.4 µm (average 2.1 × 1.0 µm), respectively. Chlamydospores that formed in chain or clusters in older cultures were brown, swollen, thick walled, and were 5.2~14.9 × 4.4~11.3 µm (average 8.9 × 8.3 µm) in size (Fig. 4C). Conidia formed either singly or in a cluster on each locus of the conidiogenous cells (Fig. 4D). The conidia were hyaline, smooth, one-celled, thin-walled, lunate, and 5.0~8.7 × 1.6~2.3 µm (average 6.4 × 1.9 µm) in size. All 10 isolates were identified as Microdochium bolleyi (Sprauge) de Hoog and Herm.-Nijh. based on their morphological and cultural characteristics (Table 1). These characteristics were consistent with previous observations (De Hoog and Hermanides-Nijhof, 1977; Von Arx, 1982).

Bottom Line: Symptoms of basal rot occurred sporadically on creeping bent grasses growing at a golf course in Hampyeong, Korea in April 2007.Ten isolates of Microdochium sp. were obtained from leaves and crowns of the diseased bent grasses.All isolates were identified as Microdochium bolleyi based on morphological, cultural, and molecular characteristics.

View Article: PubMed Central - PubMed

Affiliation: Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.

ABSTRACT
Symptoms of basal rot occurred sporadically on creeping bent grasses growing at a golf course in Hampyeong, Korea in April 2007. Ten isolates of Microdochium sp. were obtained from leaves and crowns of the diseased bent grasses. All isolates were identified as Microdochium bolleyi based on morphological, cultural, and molecular characteristics. This is the first report on M. bolleyi associated with basal rot on creeping bent grass in Korea.

No MeSH data available.