Limits...
Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

Hakoda M, Hirota Y - Bioprocess Biosyst Eng (2012)

Bottom Line: The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained.The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)].Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan. hakoda@cee.gunma-u.ac.jp

ABSTRACT
The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

Show MeSH

Related in: MedlinePlus

Schematic diagram of electric field loading device
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3755225&req=5

Fig2: Schematic diagram of electric field loading device

Mentions: An electric field loading device is shown in Fig. 2. A parallel plate electrode device was used to expose the sample cells to a uniform electric field. The device is made of pair titanium plate electrodes (100 × 100 × 1.0 mm) that sandwiched the silicon sheet with a thickness of 0.5 mm as a spacer. The shape and size of electric field loading device were necessary to get the high electric field and a lot of cells for the cultivation. The cell suspension was supplied in the device, and the electric field was applied. In the experiment that the cells were exposed to the electric fields, the electric field was applied for 10 min. The cell suspension in the device was circulated through a pipette to control the sedimentation of the cells. The device was immersed in an ice water tank to suppress the temperature rise. The medium temperature used was 278 K to prevent the deactivation of the cells.Fig. 2


Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

Hakoda M, Hirota Y - Bioprocess Biosyst Eng (2012)

Schematic diagram of electric field loading device
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755225&req=5

Fig2: Schematic diagram of electric field loading device
Mentions: An electric field loading device is shown in Fig. 2. A parallel plate electrode device was used to expose the sample cells to a uniform electric field. The device is made of pair titanium plate electrodes (100 × 100 × 1.0 mm) that sandwiched the silicon sheet with a thickness of 0.5 mm as a spacer. The shape and size of electric field loading device were necessary to get the high electric field and a lot of cells for the cultivation. The cell suspension was supplied in the device, and the electric field was applied. In the experiment that the cells were exposed to the electric fields, the electric field was applied for 10 min. The cell suspension in the device was circulated through a pipette to control the sedimentation of the cells. The device was immersed in an ice water tank to suppress the temperature rise. The medium temperature used was 278 K to prevent the deactivation of the cells.Fig. 2

Bottom Line: The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained.The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)].Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan. hakoda@cee.gunma-u.ac.jp

ABSTRACT
The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

Show MeSH
Related in: MedlinePlus