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Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

Hakoda M, Hirota Y - Bioprocess Biosyst Eng (2012)

Bottom Line: The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained.The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)].Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan. hakoda@cee.gunma-u.ac.jp

ABSTRACT
The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

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Relation between viability and Re[K(ω)] of 3-2H3 cells
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Fig13: Relation between viability and Re[K(ω)] of 3-2H3 cells

Mentions: To evaluate the cell activity by measuring the Re[K(ω)], the relation between the viability and Re[K(ω)] of the exposed cells in the DC electric field or the AC electric field was examined. The experimental results are shown in Fig. 13. The Re[K(ω)] of the cells after the AC electric field stress in this figure represents the value at 300 kHz. The viability of the cells exposed to the AC electric field decreased by decreasing Re[K(ω)]. Therefore, the correlation between the viability and the Re[K(ω)] of the exposed cells in the AC electric field was clarified. However, when the cells were cultivated for 24 h the viability was restored to more than 90 %, but the recovery of the Re[K(ω)] were low.Fig. 13


Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

Hakoda M, Hirota Y - Bioprocess Biosyst Eng (2012)

Relation between viability and Re[K(ω)] of 3-2H3 cells
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755225&req=5

Fig13: Relation between viability and Re[K(ω)] of 3-2H3 cells
Mentions: To evaluate the cell activity by measuring the Re[K(ω)], the relation between the viability and Re[K(ω)] of the exposed cells in the DC electric field or the AC electric field was examined. The experimental results are shown in Fig. 13. The Re[K(ω)] of the cells after the AC electric field stress in this figure represents the value at 300 kHz. The viability of the cells exposed to the AC electric field decreased by decreasing Re[K(ω)]. Therefore, the correlation between the viability and the Re[K(ω)] of the exposed cells in the AC electric field was clarified. However, when the cells were cultivated for 24 h the viability was restored to more than 90 %, but the recovery of the Re[K(ω)] were low.Fig. 13

Bottom Line: The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained.The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)].Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan. hakoda@cee.gunma-u.ac.jp

ABSTRACT
The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

Show MeSH
Related in: MedlinePlus