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Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

Hakoda M, Hirota Y - Bioprocess Biosyst Eng (2012)

Bottom Line: The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained.The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)].Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan. hakoda@cee.gunma-u.ac.jp

ABSTRACT
The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

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Effect of electric field on growth activity of 3-2H3 cells
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Fig10: Effect of electric field on growth activity of 3-2H3 cells

Mentions: Effects of the AC electric field or the DC electric field on the specific growth rate of the cells were examined. The specific growth rates after 24 and 48 h of the cells exposed to the electric field are shown in Fig. 10. Both specific growth rates after 24 and 48 h in cultivation of the cells exposed to the AC electric field decreased slightly compared with the cells not exposed to the electric field. At the frequency of 1 kHz, the specific growth rate decreased slightly by increasing the electric field strength. On the other hand, both the specific growth rates of the cells exposed to the DC electric field decreased dramatically after 24 and 48 h in cultivation. In addition, the specific growth rate was a negative value in the DC electric field 10 kV/m. In other words, the cells received the serious damage at the DC electric field 10 kV/m, and the cells died.Fig. 10


Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

Hakoda M, Hirota Y - Bioprocess Biosyst Eng (2012)

Effect of electric field on growth activity of 3-2H3 cells
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755225&req=5

Fig10: Effect of electric field on growth activity of 3-2H3 cells
Mentions: Effects of the AC electric field or the DC electric field on the specific growth rate of the cells were examined. The specific growth rates after 24 and 48 h of the cells exposed to the electric field are shown in Fig. 10. Both specific growth rates after 24 and 48 h in cultivation of the cells exposed to the AC electric field decreased slightly compared with the cells not exposed to the electric field. At the frequency of 1 kHz, the specific growth rate decreased slightly by increasing the electric field strength. On the other hand, both the specific growth rates of the cells exposed to the DC electric field decreased dramatically after 24 and 48 h in cultivation. In addition, the specific growth rate was a negative value in the DC electric field 10 kV/m. In other words, the cells received the serious damage at the DC electric field 10 kV/m, and the cells died.Fig. 10

Bottom Line: The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained.The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)].Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan. hakoda@cee.gunma-u.ac.jp

ABSTRACT
The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

Show MeSH
Related in: MedlinePlus