Limits...
Identification of qRBS1, a QTL involved in resistance to bacterial seedling rot in rice.

Mizobuchi R, Sato H, Fukuoka S, Tsushima S, Imbe T, Yano M - Theor. Appl. Genet. (2013)

Bottom Line: Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance.The Nona Bokra allele was associated with resistance to BSR.Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan. ritsuko@affrc.go.jp

ABSTRACT
Bacterial seedling rot (BSR), a destructive disease of rice (Oryza sativa L.), is caused by the bacterial pathogen Burkholderia glumae. To identify QTLs for resistance to BSR, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (resistant) and Koshihikari (susceptible). Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance. Further genetic analyses using an F5 population derived from a cross between a resistant CSSL and Koshihikari confirmed that a QTL for BSR resistance was located on the short arm of chromosome 10. The Nona Bokra allele was associated with resistance to BSR. Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

Show MeSH

Related in: MedlinePlus

a Bacterial seedling rot (BSR) ratios of Koshihikari, Nona Bokra, and 44 CSSLs derived from Koshihikari × Nona Bokra. The BSR ratio of each CSSL was scored 8 days after sowing of inoculated seeds. Chromosome numbers below the x axis indicate the main Nona Bokra chromosome segment contained within each CSSL. Bars indicate means, and error bars indicate SD. *Significant difference from Koshihikari at the 5 % level by Dunnett’s test. b Graphical genotypes of chromosome 10 in CSSLs containing substitutions in this chromosome. SSR markers and physical distances based on RAP-DB (IRGSP ver. 1) are indicated above the chromosome maps. White and blackbars indicate regions from Koshihikari and Nona Bokra, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3755214&req=5

Fig2: a Bacterial seedling rot (BSR) ratios of Koshihikari, Nona Bokra, and 44 CSSLs derived from Koshihikari × Nona Bokra. The BSR ratio of each CSSL was scored 8 days after sowing of inoculated seeds. Chromosome numbers below the x axis indicate the main Nona Bokra chromosome segment contained within each CSSL. Bars indicate means, and error bars indicate SD. *Significant difference from Koshihikari at the 5 % level by Dunnett’s test. b Graphical genotypes of chromosome 10 in CSSLs containing substitutions in this chromosome. SSR markers and physical distances based on RAP-DB (IRGSP ver. 1) are indicated above the chromosome maps. White and blackbars indicate regions from Koshihikari and Nona Bokra, respectively

Mentions: To identify the chromosomal segments involved in resistance to BSR, we determined the BSR ratios of the parents and the 44 CSSLs (Fig. 2a). The ratios of Koshihikari and Nona Bokra were 52.0 and 13.0 %, respectively. The BSR ratio varied widely among the CSSLs ranging from 16.6 to 67.4 %. The BSR ratios of 11 CSSLs (SL504, SL510, SL511, SL529, SL533, SL535, SL536, SL539, SL540, SL541, and SL542) were <30.0 %; two of these—SL535 and SL536—had significantly lower ratios (17.1 and 16.6 %, respectively) than the Koshihikari control (P < 0.05 by Dunnett’s test). Both had segments of chromosome 10 derived from Nona Bokra (Fig. 2b). Therefore, we hypothesized that this region of chromosome 10 might be involved in the difference in the BSR ratio between Nona Bokra and Koshihikari.Fig. 2


Identification of qRBS1, a QTL involved in resistance to bacterial seedling rot in rice.

Mizobuchi R, Sato H, Fukuoka S, Tsushima S, Imbe T, Yano M - Theor. Appl. Genet. (2013)

a Bacterial seedling rot (BSR) ratios of Koshihikari, Nona Bokra, and 44 CSSLs derived from Koshihikari × Nona Bokra. The BSR ratio of each CSSL was scored 8 days after sowing of inoculated seeds. Chromosome numbers below the x axis indicate the main Nona Bokra chromosome segment contained within each CSSL. Bars indicate means, and error bars indicate SD. *Significant difference from Koshihikari at the 5 % level by Dunnett’s test. b Graphical genotypes of chromosome 10 in CSSLs containing substitutions in this chromosome. SSR markers and physical distances based on RAP-DB (IRGSP ver. 1) are indicated above the chromosome maps. White and blackbars indicate regions from Koshihikari and Nona Bokra, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755214&req=5

Fig2: a Bacterial seedling rot (BSR) ratios of Koshihikari, Nona Bokra, and 44 CSSLs derived from Koshihikari × Nona Bokra. The BSR ratio of each CSSL was scored 8 days after sowing of inoculated seeds. Chromosome numbers below the x axis indicate the main Nona Bokra chromosome segment contained within each CSSL. Bars indicate means, and error bars indicate SD. *Significant difference from Koshihikari at the 5 % level by Dunnett’s test. b Graphical genotypes of chromosome 10 in CSSLs containing substitutions in this chromosome. SSR markers and physical distances based on RAP-DB (IRGSP ver. 1) are indicated above the chromosome maps. White and blackbars indicate regions from Koshihikari and Nona Bokra, respectively
Mentions: To identify the chromosomal segments involved in resistance to BSR, we determined the BSR ratios of the parents and the 44 CSSLs (Fig. 2a). The ratios of Koshihikari and Nona Bokra were 52.0 and 13.0 %, respectively. The BSR ratio varied widely among the CSSLs ranging from 16.6 to 67.4 %. The BSR ratios of 11 CSSLs (SL504, SL510, SL511, SL529, SL533, SL535, SL536, SL539, SL540, SL541, and SL542) were <30.0 %; two of these—SL535 and SL536—had significantly lower ratios (17.1 and 16.6 %, respectively) than the Koshihikari control (P < 0.05 by Dunnett’s test). Both had segments of chromosome 10 derived from Nona Bokra (Fig. 2b). Therefore, we hypothesized that this region of chromosome 10 might be involved in the difference in the BSR ratio between Nona Bokra and Koshihikari.Fig. 2

Bottom Line: Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance.The Nona Bokra allele was associated with resistance to BSR.Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan. ritsuko@affrc.go.jp

ABSTRACT
Bacterial seedling rot (BSR), a destructive disease of rice (Oryza sativa L.), is caused by the bacterial pathogen Burkholderia glumae. To identify QTLs for resistance to BSR, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (resistant) and Koshihikari (susceptible). Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance. Further genetic analyses using an F5 population derived from a cross between a resistant CSSL and Koshihikari confirmed that a QTL for BSR resistance was located on the short arm of chromosome 10. The Nona Bokra allele was associated with resistance to BSR. Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

Show MeSH
Related in: MedlinePlus