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Identification of qRBS1, a QTL involved in resistance to bacterial seedling rot in rice.

Mizobuchi R, Sato H, Fukuoka S, Tsushima S, Imbe T, Yano M - Theor. Appl. Genet. (2013)

Bottom Line: Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance.The Nona Bokra allele was associated with resistance to BSR.Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan. ritsuko@affrc.go.jp

ABSTRACT
Bacterial seedling rot (BSR), a destructive disease of rice (Oryza sativa L.), is caused by the bacterial pathogen Burkholderia glumae. To identify QTLs for resistance to BSR, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (resistant) and Koshihikari (susceptible). Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance. Further genetic analyses using an F5 population derived from a cross between a resistant CSSL and Koshihikari confirmed that a QTL for BSR resistance was located on the short arm of chromosome 10. The Nona Bokra allele was associated with resistance to BSR. Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

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Related in: MedlinePlus

Differences in resistance to bacterial seedling rot between Nona Bokra (left) and Koshihikari (right)
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Fig1: Differences in resistance to bacterial seedling rot between Nona Bokra (left) and Koshihikari (right)

Mentions: The BSR ratios of Koshihikari and Nona Bokra were scored 8 days after sowing (Fig. 1). Almost all of the seeds of both cultivars germinated and their shoots emerged from the soil 3–4 days after sowing. The seedlings of Koshihikari showed typical symptoms 6–8 days after sowing: some sheaths showed reddish-brown lesions and most of the others became necrotic. In contrast, most seedlings of Nona Bokra grew normally.Fig. 1


Identification of qRBS1, a QTL involved in resistance to bacterial seedling rot in rice.

Mizobuchi R, Sato H, Fukuoka S, Tsushima S, Imbe T, Yano M - Theor. Appl. Genet. (2013)

Differences in resistance to bacterial seedling rot between Nona Bokra (left) and Koshihikari (right)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755214&req=5

Fig1: Differences in resistance to bacterial seedling rot between Nona Bokra (left) and Koshihikari (right)
Mentions: The BSR ratios of Koshihikari and Nona Bokra were scored 8 days after sowing (Fig. 1). Almost all of the seeds of both cultivars germinated and their shoots emerged from the soil 3–4 days after sowing. The seedlings of Koshihikari showed typical symptoms 6–8 days after sowing: some sheaths showed reddish-brown lesions and most of the others became necrotic. In contrast, most seedlings of Nona Bokra grew normally.Fig. 1

Bottom Line: Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance.The Nona Bokra allele was associated with resistance to BSR.Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan. ritsuko@affrc.go.jp

ABSTRACT
Bacterial seedling rot (BSR), a destructive disease of rice (Oryza sativa L.), is caused by the bacterial pathogen Burkholderia glumae. To identify QTLs for resistance to BSR, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (resistant) and Koshihikari (susceptible). Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance. Further genetic analyses using an F5 population derived from a cross between a resistant CSSL and Koshihikari confirmed that a QTL for BSR resistance was located on the short arm of chromosome 10. The Nona Bokra allele was associated with resistance to BSR. Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.

Show MeSH
Related in: MedlinePlus