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Aflatoxin-mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn.

Fapohunda SO, Ezekiel CN, Alabi OA, Omole A, Chioma SO - Mycobiology (2008)

Bottom Line: Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks.The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively.PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Biotechnology, Babcock University, Ilishan Remo, Ogun State, Nigeria.

ABSTRACT
The effect of aflatoxin-contaminated corn on albino mice was investigated using the sperm morphology assay. Blood parameter levels including; total white blood cells (WBC), total red blood cells (RBC), packed cell volume (PCV), serum bilirubin (SB) and fasting blood sugar (FBS) were also determined in the tested mice. Test mice were exposed to aflatoxin-contaminated corn (contamination level of 100 ppb) for 1~4 weeks while aflatoxin-free corn and cyclophosphamide were used as negative and positive controls, respectively. Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks. The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively. PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test. Therefore, abnormal sperm cell induction is concentration-dependent such that continuous consumption of aflatoxin-contaminated corn is capable of negatively affecting spermatogenesis by inducing or increasing the frequency of morphologically abnormal sperm cells produced.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph of mice sperm cells. A, normal sperm cell; B, folded; C, amorphous head; D, pin head; E, hook at wrong angle; F, knobbed hook; G, no hook.
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Figure 2: Photomicrograph of mice sperm cells. A, normal sperm cell; B, folded; C, amorphous head; D, pin head; E, hook at wrong angle; F, knobbed hook; G, no hook.

Mentions: Sperm-head abnormalities were analyzed at 5 weeks after first day exposure to the aflatoxin-contaminated corn. Sperm cells observed at this time were presumably exposed to the aflatoxin-contaminated corn while they were early primary spermatocytes and spermatogonia. Table 1 shows the summary of the frequency of occurrence, standard deviation, mean and standard error of the different sperm abnormalities induced by varying concentrations of aflatoxin-contaminated corn. The negative control showed a percentage frequency of 18.8% while the positive control showed a statistically significant increase of 48.8%. The test concentrations showed different types of abnormal sperm heads (Fig. 2a~g). The percentage abnormalities for the different exposures of weeks 1, 2, 3 and 4 were 41.38%, 48.17%, 57.13% and 61.67%, respectively. This induction of abnormal sperm cells was concentration-dependant.


Aflatoxin-mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn.

Fapohunda SO, Ezekiel CN, Alabi OA, Omole A, Chioma SO - Mycobiology (2008)

Photomicrograph of mice sperm cells. A, normal sperm cell; B, folded; C, amorphous head; D, pin head; E, hook at wrong angle; F, knobbed hook; G, no hook.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755205&req=5

Figure 2: Photomicrograph of mice sperm cells. A, normal sperm cell; B, folded; C, amorphous head; D, pin head; E, hook at wrong angle; F, knobbed hook; G, no hook.
Mentions: Sperm-head abnormalities were analyzed at 5 weeks after first day exposure to the aflatoxin-contaminated corn. Sperm cells observed at this time were presumably exposed to the aflatoxin-contaminated corn while they were early primary spermatocytes and spermatogonia. Table 1 shows the summary of the frequency of occurrence, standard deviation, mean and standard error of the different sperm abnormalities induced by varying concentrations of aflatoxin-contaminated corn. The negative control showed a percentage frequency of 18.8% while the positive control showed a statistically significant increase of 48.8%. The test concentrations showed different types of abnormal sperm heads (Fig. 2a~g). The percentage abnormalities for the different exposures of weeks 1, 2, 3 and 4 were 41.38%, 48.17%, 57.13% and 61.67%, respectively. This induction of abnormal sperm cells was concentration-dependant.

Bottom Line: Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks.The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively.PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Biotechnology, Babcock University, Ilishan Remo, Ogun State, Nigeria.

ABSTRACT
The effect of aflatoxin-contaminated corn on albino mice was investigated using the sperm morphology assay. Blood parameter levels including; total white blood cells (WBC), total red blood cells (RBC), packed cell volume (PCV), serum bilirubin (SB) and fasting blood sugar (FBS) were also determined in the tested mice. Test mice were exposed to aflatoxin-contaminated corn (contamination level of 100 ppb) for 1~4 weeks while aflatoxin-free corn and cyclophosphamide were used as negative and positive controls, respectively. Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks. The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively. PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test. Therefore, abnormal sperm cell induction is concentration-dependent such that continuous consumption of aflatoxin-contaminated corn is capable of negatively affecting spermatogenesis by inducing or increasing the frequency of morphologically abnormal sperm cells produced.

No MeSH data available.


Related in: MedlinePlus