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Aflatoxin-mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn.

Fapohunda SO, Ezekiel CN, Alabi OA, Omole A, Chioma SO - Mycobiology (2008)

Bottom Line: Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks.The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively.PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Biotechnology, Babcock University, Ilishan Remo, Ogun State, Nigeria.

ABSTRACT
The effect of aflatoxin-contaminated corn on albino mice was investigated using the sperm morphology assay. Blood parameter levels including; total white blood cells (WBC), total red blood cells (RBC), packed cell volume (PCV), serum bilirubin (SB) and fasting blood sugar (FBS) were also determined in the tested mice. Test mice were exposed to aflatoxin-contaminated corn (contamination level of 100 ppb) for 1~4 weeks while aflatoxin-free corn and cyclophosphamide were used as negative and positive controls, respectively. Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks. The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively. PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test. Therefore, abnormal sperm cell induction is concentration-dependent such that continuous consumption of aflatoxin-contaminated corn is capable of negatively affecting spermatogenesis by inducing or increasing the frequency of morphologically abnormal sperm cells produced.

No MeSH data available.


Related in: MedlinePlus

Weight changes (grams) recorded every 4 days (T) in mice during the experiment.
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Figure 1: Weight changes (grams) recorded every 4 days (T) in mice during the experiment.

Mentions: Analysis of the weight of the mice in all the groups showed that the weight of mice for the negative control treatment increased throughout the duration of the study while all other treatment groups started decreasing after acclimatization i.e. when the contaminated corn was introduced (Fig. 1). It was observed that most of the mice had swollen limbs after being fed with contaminated corn with the exception of the negative and positive controls (data not shown).


Aflatoxin-mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn.

Fapohunda SO, Ezekiel CN, Alabi OA, Omole A, Chioma SO - Mycobiology (2008)

Weight changes (grams) recorded every 4 days (T) in mice during the experiment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755205&req=5

Figure 1: Weight changes (grams) recorded every 4 days (T) in mice during the experiment.
Mentions: Analysis of the weight of the mice in all the groups showed that the weight of mice for the negative control treatment increased throughout the duration of the study while all other treatment groups started decreasing after acclimatization i.e. when the contaminated corn was introduced (Fig. 1). It was observed that most of the mice had swollen limbs after being fed with contaminated corn with the exception of the negative and positive controls (data not shown).

Bottom Line: Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks.The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively.PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Biotechnology, Babcock University, Ilishan Remo, Ogun State, Nigeria.

ABSTRACT
The effect of aflatoxin-contaminated corn on albino mice was investigated using the sperm morphology assay. Blood parameter levels including; total white blood cells (WBC), total red blood cells (RBC), packed cell volume (PCV), serum bilirubin (SB) and fasting blood sugar (FBS) were also determined in the tested mice. Test mice were exposed to aflatoxin-contaminated corn (contamination level of 100 ppb) for 1~4 weeks while aflatoxin-free corn and cyclophosphamide were used as negative and positive controls, respectively. Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks. The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively. PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test. Therefore, abnormal sperm cell induction is concentration-dependent such that continuous consumption of aflatoxin-contaminated corn is capable of negatively affecting spermatogenesis by inducing or increasing the frequency of morphologically abnormal sperm cells produced.

No MeSH data available.


Related in: MedlinePlus