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Effect of Chitosan Acetate on Bacteria Occurring on Neungee Mushrooms, Sarcodon aspratus.

Park BS, Koo CD, Ka KH, Lee YN - Mycobiology (2008)

Bottom Line: Survival fractions of the MK1 and S strains at the MIC were 3 × 10(-4) and 1.4 × 10(-3) after 24 h, and 2 × 10(-4) and 7 × 10(-4) after 48 h, respectively.Thirty-eight percent of Neungee pieces treated in a 0.06% chitosan acetate solution for 2~3 second did not show any bacterial growth at 4 days, whereas bacterial growth around untreated mushroom pieces occurred within 2 days.These data suggest that chitosan acetate is highly effective in controlling growth of indigenous microorganisms on Neungee.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
Minimal growth inhibitory concentrations (MICs) of chitosan acetate (M.W. 60 kDa) on heterotrophic bacteria (strains MK1, S, and R) isolated from the soft-rotten tissues of Neungee mushroom (Sarcodon aspratus) were measured. The slimy substance produced by the MK1 strain was responsible for the diseased mushroom's appearance. The S and R strains were members of the Burkholderia cepacia complex. These strains showed different levels of susceptibility toward chitosan acetate. The MIC of chitosan acetate against the MK1 and S strains was 0.06%. The MIC against the R strain was greater than 0.10%. Survival fractions of the MK1 and S strains at the MIC were 3 × 10(-4) and 1.4 × 10(-3) after 24 h, and 2 × 10(-4) and 7 × 10(-4) after 48 h, respectively. Survival fractions of the R strain after 24 and 48 hr at 0.1% chitosan acetate were 1 × 10(-2) and 6.9 × 10(-3), respectively. Compared to the MK1 and S strains, the low susceptibility of the R stain towards chitosan acetate could be due to the ability of the R strain to utilize chitosan as a carbon source. Thirty-eight percent of Neungee pieces treated in a 0.06% chitosan acetate solution for 2~3 second did not show any bacterial growth at 4 days, whereas bacterial growth around untreated mushroom pieces occurred within 2 days. These data suggest that chitosan acetate is highly effective in controlling growth of indigenous microorganisms on Neungee. The scanning electron micrographs of the MK1 strain treated with chitosan revealed a higher degree of disintegrated and distorted cellular structures.

No MeSH data available.


Related in: MedlinePlus

Number of instances of bacterial growth around pileus pieces of Neungee. The pileus pieces of Neungee mushroom dipped in different concentrations of chitosan acetate were placed on PDA.
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Figure 6: Number of instances of bacterial growth around pileus pieces of Neungee. The pileus pieces of Neungee mushroom dipped in different concentrations of chitosan acetate were placed on PDA.

Mentions: There was a delay in the appearance of bacterial growth around the pieces of the freshly harvested Neungee mushroom that were dipped very briefly in chitosan solution, as shown in Fig. 5 and 6. While the control mushroom pieces (no chitosan treatment) shown 100% bacterial growth at 2 days, delayed bacterial growth was recorded on chitosan treated mushroom pieces; the higher the chitosan concentration the longer growth delay. For instance, there was no bacterial growth observed for 6 out of 16 mushroom pieces (38%) treated with 0.06% chitosan even at 4 days. This suggested that chitosan would be effective in controlling quality depreciation of Neungee mushrooms caused by the indigenous bacteria.


Effect of Chitosan Acetate on Bacteria Occurring on Neungee Mushrooms, Sarcodon aspratus.

Park BS, Koo CD, Ka KH, Lee YN - Mycobiology (2008)

Number of instances of bacterial growth around pileus pieces of Neungee. The pileus pieces of Neungee mushroom dipped in different concentrations of chitosan acetate were placed on PDA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755204&req=5

Figure 6: Number of instances of bacterial growth around pileus pieces of Neungee. The pileus pieces of Neungee mushroom dipped in different concentrations of chitosan acetate were placed on PDA.
Mentions: There was a delay in the appearance of bacterial growth around the pieces of the freshly harvested Neungee mushroom that were dipped very briefly in chitosan solution, as shown in Fig. 5 and 6. While the control mushroom pieces (no chitosan treatment) shown 100% bacterial growth at 2 days, delayed bacterial growth was recorded on chitosan treated mushroom pieces; the higher the chitosan concentration the longer growth delay. For instance, there was no bacterial growth observed for 6 out of 16 mushroom pieces (38%) treated with 0.06% chitosan even at 4 days. This suggested that chitosan would be effective in controlling quality depreciation of Neungee mushrooms caused by the indigenous bacteria.

Bottom Line: Survival fractions of the MK1 and S strains at the MIC were 3 × 10(-4) and 1.4 × 10(-3) after 24 h, and 2 × 10(-4) and 7 × 10(-4) after 48 h, respectively.Thirty-eight percent of Neungee pieces treated in a 0.06% chitosan acetate solution for 2~3 second did not show any bacterial growth at 4 days, whereas bacterial growth around untreated mushroom pieces occurred within 2 days.These data suggest that chitosan acetate is highly effective in controlling growth of indigenous microorganisms on Neungee.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
Minimal growth inhibitory concentrations (MICs) of chitosan acetate (M.W. 60 kDa) on heterotrophic bacteria (strains MK1, S, and R) isolated from the soft-rotten tissues of Neungee mushroom (Sarcodon aspratus) were measured. The slimy substance produced by the MK1 strain was responsible for the diseased mushroom's appearance. The S and R strains were members of the Burkholderia cepacia complex. These strains showed different levels of susceptibility toward chitosan acetate. The MIC of chitosan acetate against the MK1 and S strains was 0.06%. The MIC against the R strain was greater than 0.10%. Survival fractions of the MK1 and S strains at the MIC were 3 × 10(-4) and 1.4 × 10(-3) after 24 h, and 2 × 10(-4) and 7 × 10(-4) after 48 h, respectively. Survival fractions of the R strain after 24 and 48 hr at 0.1% chitosan acetate were 1 × 10(-2) and 6.9 × 10(-3), respectively. Compared to the MK1 and S strains, the low susceptibility of the R stain towards chitosan acetate could be due to the ability of the R strain to utilize chitosan as a carbon source. Thirty-eight percent of Neungee pieces treated in a 0.06% chitosan acetate solution for 2~3 second did not show any bacterial growth at 4 days, whereas bacterial growth around untreated mushroom pieces occurred within 2 days. These data suggest that chitosan acetate is highly effective in controlling growth of indigenous microorganisms on Neungee. The scanning electron micrographs of the MK1 strain treated with chitosan revealed a higher degree of disintegrated and distorted cellular structures.

No MeSH data available.


Related in: MedlinePlus