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Two Species of Endophytic Penicillium from Pinus rigida in Korea.

Kim CS, Park MS, Yu SH - Mycobiology (2008)

Bottom Line: Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered.In this study, we report two species of Penicillium isolated from the needles of Pinus rigida.Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea.

ABSTRACT
Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered. In this study, we report two species of Penicillium isolated from the needles of Pinus rigida. Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.

No MeSH data available.


One of sixth-seven parsimonious trees inferred from sequence of ID region. The numbers above each branch indicate bootstrap values obtained after a bootstrap test with 1000 replications. The bar represents 0.01 substitutions per site. Numbers in parentheses are GenBank accession numbers.
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Figure 1: One of sixth-seven parsimonious trees inferred from sequence of ID region. The numbers above each branch indicate bootstrap values obtained after a bootstrap test with 1000 replications. The bar represents 0.01 substitutions per site. Numbers in parentheses are GenBank accession numbers.

Mentions: The ITS and lsu-rDNA (ID) from both Penicillium isolates from Pinus rigida in Korea were amplified. Amplification of the ID region with primers ITS1 and D2R yielded a fragment of approximately 1120 bp and alignment of the sequences resulted in a dataset of 1156 characters (27.1% variable and 20.9% parsimony informative characters). Parsimony analysis yielded sixth-seven parsimonious trees of 668 steps (CI = 0.6189, RI = 0.8293) (Fig. 1). In the maximum parsimony analysis, CNU 060078 isolate, P. fellutanum NRRL 746 and P. charlesii NRRL 778 were clustered together in a group, which was supported by a bootstrap value of 89%. The ID region sequence of CNU 060078 was identical to that of neotype of P. fellutanum NRRL 746 (Fig. 1).


Two Species of Endophytic Penicillium from Pinus rigida in Korea.

Kim CS, Park MS, Yu SH - Mycobiology (2008)

One of sixth-seven parsimonious trees inferred from sequence of ID region. The numbers above each branch indicate bootstrap values obtained after a bootstrap test with 1000 replications. The bar represents 0.01 substitutions per site. Numbers in parentheses are GenBank accession numbers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755199&req=5

Figure 1: One of sixth-seven parsimonious trees inferred from sequence of ID region. The numbers above each branch indicate bootstrap values obtained after a bootstrap test with 1000 replications. The bar represents 0.01 substitutions per site. Numbers in parentheses are GenBank accession numbers.
Mentions: The ITS and lsu-rDNA (ID) from both Penicillium isolates from Pinus rigida in Korea were amplified. Amplification of the ID region with primers ITS1 and D2R yielded a fragment of approximately 1120 bp and alignment of the sequences resulted in a dataset of 1156 characters (27.1% variable and 20.9% parsimony informative characters). Parsimony analysis yielded sixth-seven parsimonious trees of 668 steps (CI = 0.6189, RI = 0.8293) (Fig. 1). In the maximum parsimony analysis, CNU 060078 isolate, P. fellutanum NRRL 746 and P. charlesii NRRL 778 were clustered together in a group, which was supported by a bootstrap value of 89%. The ID region sequence of CNU 060078 was identical to that of neotype of P. fellutanum NRRL 746 (Fig. 1).

Bottom Line: Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered.In this study, we report two species of Penicillium isolated from the needles of Pinus rigida.Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea.

ABSTRACT
Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered. In this study, we report two species of Penicillium isolated from the needles of Pinus rigida. Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.

No MeSH data available.