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Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.

Vu VH, Hong SI, Kim K - Mycobiology (2008)

Bottom Line: The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids.The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time.The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea.

ABSTRACT
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

No MeSH data available.


Related in: MedlinePlus

Effect of rice pH on aerial conidia production. The inoculated steamed polished rice with different pHs and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 12 days.
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Figure 5: Effect of rice pH on aerial conidia production. The inoculated steamed polished rice with different pHs and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 12 days.

Mentions: pH is one of the factors that influences microbial development during SSF (Doelle, 1985). The results depicted in Fig. 5 indicated that rice pH markedly affected aerial conidia production of L. lecanii 41185 (P < 0.01). Rice pH from 5.5 to 6.0 was optimal for the highest conidia production at 6.45 × 109 and 6.47 × 109 conidia/g polished rice, respectively, after 12 d of cultivation at 25℃. Beyond the range of optimal pH (5.5~6.0) the production of aerial conidia of L. lecanii 41185 declined sharply.


Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.

Vu VH, Hong SI, Kim K - Mycobiology (2008)

Effect of rice pH on aerial conidia production. The inoculated steamed polished rice with different pHs and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 12 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755191&req=5

Figure 5: Effect of rice pH on aerial conidia production. The inoculated steamed polished rice with different pHs and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 12 days.
Mentions: pH is one of the factors that influences microbial development during SSF (Doelle, 1985). The results depicted in Fig. 5 indicated that rice pH markedly affected aerial conidia production of L. lecanii 41185 (P < 0.01). Rice pH from 5.5 to 6.0 was optimal for the highest conidia production at 6.45 × 109 and 6.47 × 109 conidia/g polished rice, respectively, after 12 d of cultivation at 25℃. Beyond the range of optimal pH (5.5~6.0) the production of aerial conidia of L. lecanii 41185 declined sharply.

Bottom Line: The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids.The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time.The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea.

ABSTRACT
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

No MeSH data available.


Related in: MedlinePlus