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Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.

Vu VH, Hong SI, Kim K - Mycobiology (2008)

Bottom Line: The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids.The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time.The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea.

ABSTRACT
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

No MeSH data available.


Related in: MedlinePlus

Effect of cultivation time on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.
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Figure 3: Effect of cultivation time on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.

Mentions: The conidial yield significantly depended on the culture time as shown in Fig. 3. The longer the incubation time, the higher the conidia yield. Thus by using steamed polished rice containing 28.5% moisture, the conidia yield was 6.13 × 109 conidia/g polished rice after 12 d of cultivation. Further cultivation after 12 d did not increase the conidial number.


Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.

Vu VH, Hong SI, Kim K - Mycobiology (2008)

Effect of cultivation time on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755191&req=5

Figure 3: Effect of cultivation time on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.
Mentions: The conidial yield significantly depended on the culture time as shown in Fig. 3. The longer the incubation time, the higher the conidia yield. Thus by using steamed polished rice containing 28.5% moisture, the conidia yield was 6.13 × 109 conidia/g polished rice after 12 d of cultivation. Further cultivation after 12 d did not increase the conidial number.

Bottom Line: The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids.The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time.The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea.

ABSTRACT
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

No MeSH data available.


Related in: MedlinePlus