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Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.

Vu VH, Hong SI, Kim K - Mycobiology (2008)

Bottom Line: The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids.The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time.The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea.

ABSTRACT
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

No MeSH data available.


Related in: MedlinePlus

Effect of rice moisture content on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.
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Figure 2: Effect of rice moisture content on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.

Mentions: Moisture content in the substrate plays a significant role in the final yield of conidia (Jenkins et al., 1998). Most mitosporic fungi prefer humid conditions; however, different substrates vary in their moisture sorption curves and will reach maximum adsorption at different moisture content levels. In this study, moisture content in rice was depended on the draining time of water from the soaked substrate; where less draining time resulted in increased water content of the rice. The production of aerial conidia of L. lecanii 41185 drastically depended on both moisture content and culture time (P < 0.001) for the duration of conidia production, as depicted in Fig. 2. The conidial yield increased with the length of incubation time. The conidia number produced on rice containing 28.5% moisture was higher than those at other moisture contents, over any culture time, and the highest amount of conidia was 6.13 × 109 conidia/g polished rice at 12 days after inoculation. The amount of conidia was the lowest on the rice containing 23.5% moisture.


Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.

Vu VH, Hong SI, Kim K - Mycobiology (2008)

Effect of rice moisture content on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755191&req=5

Figure 2: Effect of rice moisture content on aerial conidia production. The inoculated steamed polished rice with a pH of 6.5 and 28.5% moisture content was incubated at 25℃ and 50~60% RH for 7~12 days.
Mentions: Moisture content in the substrate plays a significant role in the final yield of conidia (Jenkins et al., 1998). Most mitosporic fungi prefer humid conditions; however, different substrates vary in their moisture sorption curves and will reach maximum adsorption at different moisture content levels. In this study, moisture content in rice was depended on the draining time of water from the soaked substrate; where less draining time resulted in increased water content of the rice. The production of aerial conidia of L. lecanii 41185 drastically depended on both moisture content and culture time (P < 0.001) for the duration of conidia production, as depicted in Fig. 2. The conidial yield increased with the length of incubation time. The conidia number produced on rice containing 28.5% moisture was higher than those at other moisture contents, over any culture time, and the highest amount of conidia was 6.13 × 109 conidia/g polished rice at 12 days after inoculation. The amount of conidia was the lowest on the rice containing 23.5% moisture.

Bottom Line: The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids.The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time.The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea.

ABSTRACT
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25℃ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 × 10(9) conidia/g polished rice to 18.2 × 10(9) conidia/g polished rice following application of these optimized conditions.

No MeSH data available.


Related in: MedlinePlus