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Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides.

Lee JW, Baek SJ, Kim YS - Mycobiology (2008)

Bottom Line: The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4·7H2O, 0.50 g/l; FeCl2·62O, 0.01 g/l; MnCl2·4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4·7H2O, 0.005 g/l.The optimal culture conditions were determined to be as follows: temperature, 28℃; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively.Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Processing and Cooking, Kyungbuk College, Yeongjusi 750-712, Korea.

ABSTRACT
In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4·7H2O, 0.50 g/l; FeCl2·62O, 0.01 g/l; MnCl2·4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4·7H2O, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28℃; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

No MeSH data available.


Related in: MedlinePlus

Mycelial growth and fructose consumption from Phellinus linteus in a 5 l fermentor. Mycelial weight (•), Residual fructose (○), Viscosity (▪), pH (□).
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Figure 1: Mycelial growth and fructose consumption from Phellinus linteus in a 5 l fermentor. Mycelial weight (•), Residual fructose (○), Viscosity (▪), pH (□).

Mentions: Fig. 1 shows the typical time courses of mycelial growth and fructose consumption in a 5 l jar fermentor. Under optimum culture conditions, the maximum mycelial production rate was 29.9 g/l at 144 h of fermentation. To the best of our knowledge, the mycelial production obtained in this study is the highest yield amongst liquid cultures of P. linteus reported in the literature. The apparent viscosity of the fermentation broth of P. linteus IY003 increased rapidly during the exponential phase, but a rapid decrease was detected in a later stage. This drop in viscosity may be attributable to the occurrence of severe fragmentation in mycelial clumps of P. linteus IY003. Based on the results reported by another investigator, the apparent viscosity was influenced by mycelial fragmentation (Hwang et al., 2004).


Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides.

Lee JW, Baek SJ, Kim YS - Mycobiology (2008)

Mycelial growth and fructose consumption from Phellinus linteus in a 5 l fermentor. Mycelial weight (•), Residual fructose (○), Viscosity (▪), pH (□).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755190&req=5

Figure 1: Mycelial growth and fructose consumption from Phellinus linteus in a 5 l fermentor. Mycelial weight (•), Residual fructose (○), Viscosity (▪), pH (□).
Mentions: Fig. 1 shows the typical time courses of mycelial growth and fructose consumption in a 5 l jar fermentor. Under optimum culture conditions, the maximum mycelial production rate was 29.9 g/l at 144 h of fermentation. To the best of our knowledge, the mycelial production obtained in this study is the highest yield amongst liquid cultures of P. linteus reported in the literature. The apparent viscosity of the fermentation broth of P. linteus IY003 increased rapidly during the exponential phase, but a rapid decrease was detected in a later stage. This drop in viscosity may be attributable to the occurrence of severe fragmentation in mycelial clumps of P. linteus IY003. Based on the results reported by another investigator, the apparent viscosity was influenced by mycelial fragmentation (Hwang et al., 2004).

Bottom Line: The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4·7H2O, 0.50 g/l; FeCl2·62O, 0.01 g/l; MnCl2·4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4·7H2O, 0.005 g/l.The optimal culture conditions were determined to be as follows: temperature, 28℃; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively.Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Processing and Cooking, Kyungbuk College, Yeongjusi 750-712, Korea.

ABSTRACT
In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4·7H2O, 0.50 g/l; FeCl2·62O, 0.01 g/l; MnCl2·4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4·7H2O, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28℃; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

No MeSH data available.


Related in: MedlinePlus