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Identification of Diachea leucopodia on Strawberry from Greenhouse in Korea.

Lee JH, Han KS, Bae DW, Kim DK, Kim HK - Mycobiology (2008)

Bottom Line: The above characteristics confirm its taxonomic position in the genus Diachea.Hence, this genus had been classified as a member of the Stemonitales until the mid-1970's, on the basis of its iridescent peridium and noncalcareous capillitial system, similar to Comatricha of the Stemonitaceae.The presence of a phaneroplasmodium during the trophic stage and lime deposition in its sporophores, as was confirmed in this work, supported the inclusion of Diachea in the Physarales, and the noncalcareous capillitial system verified its identification as a member of the Didymiaceae.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biology & Environmental Sciences, Gyeongsang National University, Jinju 660-701, Korea.

ABSTRACT
We have detected the slime mold, Diachea leucopodia (GNU06-10) in a strawberry greenhouse located in Sancheong-gun, Gyeongnam. Typical fruiting bodies had developed gregariously on the strawberry leaves, petioles, and plant debris on ground soil habitat, and also surprisingly on plastic pipes and a vinyl covering. Field samples were examined via stereomicroscopy, light microscopy, and SEM for the determination of morphological characteristics. Dark-brown to black spores formed gregariously within the stipitate cylindrical sporangium, and were covered by an iridescent peridium, which may be intact at maturity, or may have disintegrated. The upper portion of the peridium generally breaks up to expose the spores, whereas the lower portion was usually persistent. The results of energy dispersive X-ray spectrometer (EDS) analysis showed that lime was present in the stalk and columella but absent from the spores, capillitium, and peridium. The above characteristics confirm its taxonomic position in the genus Diachea. However, this genus is intermediate in character between the Physarales and Stemonitales of the Myxogastromycetidae. Hence, this genus had been classified as a member of the Stemonitales until the mid-1970's, on the basis of its iridescent peridium and noncalcareous capillitial system, similar to Comatricha of the Stemonitaceae. By way of contrast, emphasis on morphological characteristics, most notably the calcareous stalk and typical columella, places Diachea within the order Physarales. The presence of a phaneroplasmodium during the trophic stage and lime deposition in its sporophores, as was confirmed in this work, supported the inclusion of Diachea in the Physarales, and the noncalcareous capillitial system verified its identification as a member of the Didymiaceae. Further characteristics of the species D. leucopodia include the following: phaneroplasmodium, spore globose 7.5 µm in diameter, very minutely roughened; sporangia 500 µm × 1mm, more or less cylindrical, gregarious, stalked 1.2mm; stalk and columella white.

No MeSH data available.


Related in: MedlinePlus

EDS spectra of element deposition for spore against stalk or spore against carbon ribbon. Portion of SEM view shown as a tetragon was subjected to EDS analysis (SPs) and EDS digital Ca mapping (SPm). SPs-1 shows the EDS spectrum for spores adsorbed on a carbon ribbon. LE, Longitudinal extrusion of membrane wrinkled and folded up due to desiccation.
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Figure 2: EDS spectra of element deposition for spore against stalk or spore against carbon ribbon. Portion of SEM view shown as a tetragon was subjected to EDS analysis (SPs) and EDS digital Ca mapping (SPm). SPs-1 shows the EDS spectrum for spores adsorbed on a carbon ribbon. LE, Longitudinal extrusion of membrane wrinkled and folded up due to desiccation.

Mentions: The morphology of fruiting bodies that developed on the strawberry plants and ground habitat is described in Fig. 1A and B. The FESEM micrograph in Fig. 1C shows the cylindrical sporangia; globose, very minutely echinulated (Fig. 2) spores, capillitium, columella, single peridium, stalk and hypothallus. Further relevant characteristics, in detail, are as follows. Sporangia: stipitate, closely gregarious, cylindric, ochre to grayish black, apex obtuse, base slightly umbilicate or navel-shaped, total height 2.2 mm, up to 0.5 mm broad; peridium single, membranous, iridescent distinct in paler peridium, the upper portion dehisce to expose the spores while the lower portion remains persistent; stalk long, up to 1.2 mm in height, chalk white, looks very prominent against the black sporangium, broader at the base, tapering towards the apex, striated; hypothallus white, not very well developed. Columella: an elongation of the stipe, thick, blunt, white, calcareous, tapering, reaching two-thirds to the apex. Capillitium: profuse, anastomosing, flexuous threads, white. Spores: dark-brown to black, medium-brown in shade in transmitted light, globose, very minutely roughened, 7.5 µm in diameter.


Identification of Diachea leucopodia on Strawberry from Greenhouse in Korea.

Lee JH, Han KS, Bae DW, Kim DK, Kim HK - Mycobiology (2008)

EDS spectra of element deposition for spore against stalk or spore against carbon ribbon. Portion of SEM view shown as a tetragon was subjected to EDS analysis (SPs) and EDS digital Ca mapping (SPm). SPs-1 shows the EDS spectrum for spores adsorbed on a carbon ribbon. LE, Longitudinal extrusion of membrane wrinkled and folded up due to desiccation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755183&req=5

Figure 2: EDS spectra of element deposition for spore against stalk or spore against carbon ribbon. Portion of SEM view shown as a tetragon was subjected to EDS analysis (SPs) and EDS digital Ca mapping (SPm). SPs-1 shows the EDS spectrum for spores adsorbed on a carbon ribbon. LE, Longitudinal extrusion of membrane wrinkled and folded up due to desiccation.
Mentions: The morphology of fruiting bodies that developed on the strawberry plants and ground habitat is described in Fig. 1A and B. The FESEM micrograph in Fig. 1C shows the cylindrical sporangia; globose, very minutely echinulated (Fig. 2) spores, capillitium, columella, single peridium, stalk and hypothallus. Further relevant characteristics, in detail, are as follows. Sporangia: stipitate, closely gregarious, cylindric, ochre to grayish black, apex obtuse, base slightly umbilicate or navel-shaped, total height 2.2 mm, up to 0.5 mm broad; peridium single, membranous, iridescent distinct in paler peridium, the upper portion dehisce to expose the spores while the lower portion remains persistent; stalk long, up to 1.2 mm in height, chalk white, looks very prominent against the black sporangium, broader at the base, tapering towards the apex, striated; hypothallus white, not very well developed. Columella: an elongation of the stipe, thick, blunt, white, calcareous, tapering, reaching two-thirds to the apex. Capillitium: profuse, anastomosing, flexuous threads, white. Spores: dark-brown to black, medium-brown in shade in transmitted light, globose, very minutely roughened, 7.5 µm in diameter.

Bottom Line: The above characteristics confirm its taxonomic position in the genus Diachea.Hence, this genus had been classified as a member of the Stemonitales until the mid-1970's, on the basis of its iridescent peridium and noncalcareous capillitial system, similar to Comatricha of the Stemonitaceae.The presence of a phaneroplasmodium during the trophic stage and lime deposition in its sporophores, as was confirmed in this work, supported the inclusion of Diachea in the Physarales, and the noncalcareous capillitial system verified its identification as a member of the Didymiaceae.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biology & Environmental Sciences, Gyeongsang National University, Jinju 660-701, Korea.

ABSTRACT
We have detected the slime mold, Diachea leucopodia (GNU06-10) in a strawberry greenhouse located in Sancheong-gun, Gyeongnam. Typical fruiting bodies had developed gregariously on the strawberry leaves, petioles, and plant debris on ground soil habitat, and also surprisingly on plastic pipes and a vinyl covering. Field samples were examined via stereomicroscopy, light microscopy, and SEM for the determination of morphological characteristics. Dark-brown to black spores formed gregariously within the stipitate cylindrical sporangium, and were covered by an iridescent peridium, which may be intact at maturity, or may have disintegrated. The upper portion of the peridium generally breaks up to expose the spores, whereas the lower portion was usually persistent. The results of energy dispersive X-ray spectrometer (EDS) analysis showed that lime was present in the stalk and columella but absent from the spores, capillitium, and peridium. The above characteristics confirm its taxonomic position in the genus Diachea. However, this genus is intermediate in character between the Physarales and Stemonitales of the Myxogastromycetidae. Hence, this genus had been classified as a member of the Stemonitales until the mid-1970's, on the basis of its iridescent peridium and noncalcareous capillitial system, similar to Comatricha of the Stemonitaceae. By way of contrast, emphasis on morphological characteristics, most notably the calcareous stalk and typical columella, places Diachea within the order Physarales. The presence of a phaneroplasmodium during the trophic stage and lime deposition in its sporophores, as was confirmed in this work, supported the inclusion of Diachea in the Physarales, and the noncalcareous capillitial system verified its identification as a member of the Didymiaceae. Further characteristics of the species D. leucopodia include the following: phaneroplasmodium, spore globose 7.5 µm in diameter, very minutely roughened; sporangia 500 µm × 1mm, more or less cylindrical, gregarious, stalked 1.2mm; stalk and columella white.

No MeSH data available.


Related in: MedlinePlus