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Evaluation of a novel thermosensitive heparin-poloxamer hydrogel for improving vascular anastomosis quality and safety in a rabbit model.

Zhao YZ, Lv HF, Lu CT, Chen LJ, Lin M, Zhang M, Jiang X, Shen XT, Jin RR, Cai J, Tian XQ, Wong HL - PLoS ONE (2013)

Bottom Line: Our results showed that HP copolymer displayed special gel-sol-gel phase transition behavior with increasing temperature from 5 to 60 °C.In animal studies, imaging and histological examination of rabbit common jugular artery confirmed that HP hydrogel group had similar equivalent patency, flow and burst strength as Poloxamer 407 group.Moreover, HP hydrogel was superior to poloxamer 407 hydrogel and hand-sewn method for restoring the functions and epithelial structure of the broken vessel junctions after operation.

View Article: PubMed Central - PubMed

Affiliation: Wenzhou Medical University, Wenzhou City, Zhejiang Province, China ; College of Medicine, Drexel University, Philadelphia, Pennsylvania, United States of America.

ABSTRACT
Despite progress in the design of advanced surgical techniques, stenosis recurs in a large percentage of vascular anastomosis. In this study, a novel heparin-poloxamer (HP) hydrogel was designed and its effects for improving the quality and safety of vascular anastomosis were studied. HP copolymer was synthesized and its structure was confirmed by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy ((1)H-NMR). Hydrogels containing HP were prepared and their important characteristics related to the application in vascular anastomosis including gelation temperature, rheological behaviour and micromorphology were measured. Vascular anastomosis were performed on the right common carotid arteries of rabbits, and the in vivo efficiency and safety of HP hydrogel to achieve vascular anastomosis was verified and compared with Poloxamer 407 hydrogel and the conventional hand-sewn method using Doppler ultrasound, CT angiograms, scanning electron microscopy (SEM) and histological technique. Our results showed that HP copolymer displayed special gel-sol-gel phase transition behavior with increasing temperature from 5 to 60 °C. HP hydrogel prepared from 18 wt% HP solution had a porous sponge-like structure, with gelation temperature at approximately 38 °C and maximum elastic modulus at 10,000 Pa. In animal studies, imaging and histological examination of rabbit common jugular artery confirmed that HP hydrogel group had similar equivalent patency, flow and burst strength as Poloxamer 407 group. Moreover, HP hydrogel was superior to poloxamer 407 hydrogel and hand-sewn method for restoring the functions and epithelial structure of the broken vessel junctions after operation. By combining the advantages of heparin and poloxamer 407, HP hydrogel holds high promise for improving vascular anastomosis quality and safety.

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Related in: MedlinePlus

Postoperative CT angiograms, ultrasound Doppler images, and scanning electron microscopes of experimental groups.(A: Postoperative CT angiograms; B: Ultrasound Doppler images performed at 6 weeks after operation; C: Scanning electron microscope of different vascular anastomosis after 12 weeks. a: Sham-operated group, b: hand-sewn group, c: poloxamer 407 group, d: HP hydrogel group).
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pone-0073178-g005: Postoperative CT angiograms, ultrasound Doppler images, and scanning electron microscopes of experimental groups.(A: Postoperative CT angiograms; B: Ultrasound Doppler images performed at 6 weeks after operation; C: Scanning electron microscope of different vascular anastomosis after 12 weeks. a: Sham-operated group, b: hand-sewn group, c: poloxamer 407 group, d: HP hydrogel group).

Mentions: In order to evaluate the recanalization of blood flow at the jugular artery after vascular anastomosis, sixteen slice spiral CT and Ultrasound Doppler were used in this experiment. As shown in Figure 5A, postoperative CT angiograms performed at 6 weeks confirmed equivalent anastomoses using the poloxamer 407, HP hydrogel and hand-sewn techniques to sites of anastomoses. Little differences were found in the postoperative CT angiograms of the three groups (P > 0.05). Ultrasound Doppler studies also showed no observable differences in vessel diameter (2.1± 0.23 mm versus 2.2 ± 0.19 mm, P > 0.05) and confirmed patency of all anastomoses with similar volumetric flow rates (hand-sewn group: 0.225 ± 0.005 m/s, poloxamer 407 group: 0.236 ± 0.021 m/s, HP hydrogel group: 0.251 ± 0.324m/s, P > 0.05) (Figure 5B).


Evaluation of a novel thermosensitive heparin-poloxamer hydrogel for improving vascular anastomosis quality and safety in a rabbit model.

Zhao YZ, Lv HF, Lu CT, Chen LJ, Lin M, Zhang M, Jiang X, Shen XT, Jin RR, Cai J, Tian XQ, Wong HL - PLoS ONE (2013)

Postoperative CT angiograms, ultrasound Doppler images, and scanning electron microscopes of experimental groups.(A: Postoperative CT angiograms; B: Ultrasound Doppler images performed at 6 weeks after operation; C: Scanning electron microscope of different vascular anastomosis after 12 weeks. a: Sham-operated group, b: hand-sewn group, c: poloxamer 407 group, d: HP hydrogel group).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755001&req=5

pone-0073178-g005: Postoperative CT angiograms, ultrasound Doppler images, and scanning electron microscopes of experimental groups.(A: Postoperative CT angiograms; B: Ultrasound Doppler images performed at 6 weeks after operation; C: Scanning electron microscope of different vascular anastomosis after 12 weeks. a: Sham-operated group, b: hand-sewn group, c: poloxamer 407 group, d: HP hydrogel group).
Mentions: In order to evaluate the recanalization of blood flow at the jugular artery after vascular anastomosis, sixteen slice spiral CT and Ultrasound Doppler were used in this experiment. As shown in Figure 5A, postoperative CT angiograms performed at 6 weeks confirmed equivalent anastomoses using the poloxamer 407, HP hydrogel and hand-sewn techniques to sites of anastomoses. Little differences were found in the postoperative CT angiograms of the three groups (P > 0.05). Ultrasound Doppler studies also showed no observable differences in vessel diameter (2.1± 0.23 mm versus 2.2 ± 0.19 mm, P > 0.05) and confirmed patency of all anastomoses with similar volumetric flow rates (hand-sewn group: 0.225 ± 0.005 m/s, poloxamer 407 group: 0.236 ± 0.021 m/s, HP hydrogel group: 0.251 ± 0.324m/s, P > 0.05) (Figure 5B).

Bottom Line: Our results showed that HP copolymer displayed special gel-sol-gel phase transition behavior with increasing temperature from 5 to 60 °C.In animal studies, imaging and histological examination of rabbit common jugular artery confirmed that HP hydrogel group had similar equivalent patency, flow and burst strength as Poloxamer 407 group.Moreover, HP hydrogel was superior to poloxamer 407 hydrogel and hand-sewn method for restoring the functions and epithelial structure of the broken vessel junctions after operation.

View Article: PubMed Central - PubMed

Affiliation: Wenzhou Medical University, Wenzhou City, Zhejiang Province, China ; College of Medicine, Drexel University, Philadelphia, Pennsylvania, United States of America.

ABSTRACT
Despite progress in the design of advanced surgical techniques, stenosis recurs in a large percentage of vascular anastomosis. In this study, a novel heparin-poloxamer (HP) hydrogel was designed and its effects for improving the quality and safety of vascular anastomosis were studied. HP copolymer was synthesized and its structure was confirmed by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy ((1)H-NMR). Hydrogels containing HP were prepared and their important characteristics related to the application in vascular anastomosis including gelation temperature, rheological behaviour and micromorphology were measured. Vascular anastomosis were performed on the right common carotid arteries of rabbits, and the in vivo efficiency and safety of HP hydrogel to achieve vascular anastomosis was verified and compared with Poloxamer 407 hydrogel and the conventional hand-sewn method using Doppler ultrasound, CT angiograms, scanning electron microscopy (SEM) and histological technique. Our results showed that HP copolymer displayed special gel-sol-gel phase transition behavior with increasing temperature from 5 to 60 °C. HP hydrogel prepared from 18 wt% HP solution had a porous sponge-like structure, with gelation temperature at approximately 38 °C and maximum elastic modulus at 10,000 Pa. In animal studies, imaging and histological examination of rabbit common jugular artery confirmed that HP hydrogel group had similar equivalent patency, flow and burst strength as Poloxamer 407 group. Moreover, HP hydrogel was superior to poloxamer 407 hydrogel and hand-sewn method for restoring the functions and epithelial structure of the broken vessel junctions after operation. By combining the advantages of heparin and poloxamer 407, HP hydrogel holds high promise for improving vascular anastomosis quality and safety.

Show MeSH
Related in: MedlinePlus