Limits...
Growing poplars for research with and without mycorrhizas.

Müller A, Volmer K, Mishra-Knyrim M, Polle A - Front Plant Sci (2013)

Bottom Line: The basis of these investigations is the reproducible production of homogeneous plant material.Maintenance and plant preparation require different multiplication and rooting media.Growth and vitality of the trees in vitro and outdoors with and without ectomycorrhizas are reported.

View Article: PubMed Central - PubMed

Affiliation: Forest Botany and Tree Physiology, Büsgen-Institut, Georg-August Universität Göttingen Göttingen, Germany.

ABSTRACT
During the last decades the importance of the genus Populus increased because the poplar genome has been sequenced and molecular tools for basic research have become available. Poplar species occur in different habitats and harbor large genetic variation, which can be exploited for economic applications and for increasing our knowledge on the basic molecular mechanisms of the woody life style. Poplars are, therefore, employed to unravel the molecular mechanisms of wood formation, stress tolerance, tree nutrition and interaction with other organisms such as pathogens or mycorrhiza. The basis of these investigations is the reproducible production of homogeneous plant material. In this method paper we describe techniques and growth conditions for the in vitro propagation of different poplar species (Populus × canescens, P. trichocarpa, P. tremula, and P. euphratica) and ectomycorrhizal fungi (Laccaria bicolor, Paxillus involutus) as well as for their co-cultivation for ectomycorrhizal synthesis. Maintenance and plant preparation require different multiplication and rooting media. Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are described. Transfer of the plants from in vitro to in situ conditions is critical and hardening is important to prevent high mortality. Growth and vitality of the trees in vitro and outdoors with and without ectomycorrhizas are reported.

No MeSH data available.


Related in: MedlinePlus

Cocultivation of Populus × canescens with L. bicolor under axenic conditions. (A) Preculture of L. bicolor in a Petri dish on a cellophane membrane on P20 medium. It is possible to equip this system with two membranes in one Petri dish. (B) Cocultivation of P. × canescens with L. bicolor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3753594&req=5

Figure 5: Cocultivation of Populus × canescens with L. bicolor under axenic conditions. (A) Preculture of L. bicolor in a Petri dish on a cellophane membrane on P20 medium. It is possible to equip this system with two membranes in one Petri dish. (B) Cocultivation of P. × canescens with L. bicolor.

Mentions: Square Petri dishes (12 × 12 cm) were filled with 50 ml sugar-reduced P20 medium. Cellophane membranes were cut into halves (6 × 12 cm). The membranes were first boiled twice for 30 min in distilled water and subsequently autoclaved twice. Two membranes were placed in one Petri dish onto the P20 medium next to each other (Figure 5A). Eleven fungal plugs of Laccaria bicolor cultures, which had been pre-cultured on P05 media, were placed on the membrane (Figure 5A). The Petri dishes were sealed with Parafilm and kept at 23°C in permanent darkness. The regular placement of the fungal plugs on the cellophane membranes ensured the regular coverage of the cellophane surface with actively growing fungal mycelium after 10 days of culture. The arrangement on the cellophane membrane as well as the precultivation time should be adjusted according to the growth of the fungus. It is therefore recommended to analyze the growth of the fungus on the used media before starting the final experiment.


Growing poplars for research with and without mycorrhizas.

Müller A, Volmer K, Mishra-Knyrim M, Polle A - Front Plant Sci (2013)

Cocultivation of Populus × canescens with L. bicolor under axenic conditions. (A) Preculture of L. bicolor in a Petri dish on a cellophane membrane on P20 medium. It is possible to equip this system with two membranes in one Petri dish. (B) Cocultivation of P. × canescens with L. bicolor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3753594&req=5

Figure 5: Cocultivation of Populus × canescens with L. bicolor under axenic conditions. (A) Preculture of L. bicolor in a Petri dish on a cellophane membrane on P20 medium. It is possible to equip this system with two membranes in one Petri dish. (B) Cocultivation of P. × canescens with L. bicolor.
Mentions: Square Petri dishes (12 × 12 cm) were filled with 50 ml sugar-reduced P20 medium. Cellophane membranes were cut into halves (6 × 12 cm). The membranes were first boiled twice for 30 min in distilled water and subsequently autoclaved twice. Two membranes were placed in one Petri dish onto the P20 medium next to each other (Figure 5A). Eleven fungal plugs of Laccaria bicolor cultures, which had been pre-cultured on P05 media, were placed on the membrane (Figure 5A). The Petri dishes were sealed with Parafilm and kept at 23°C in permanent darkness. The regular placement of the fungal plugs on the cellophane membranes ensured the regular coverage of the cellophane surface with actively growing fungal mycelium after 10 days of culture. The arrangement on the cellophane membrane as well as the precultivation time should be adjusted according to the growth of the fungus. It is therefore recommended to analyze the growth of the fungus on the used media before starting the final experiment.

Bottom Line: The basis of these investigations is the reproducible production of homogeneous plant material.Maintenance and plant preparation require different multiplication and rooting media.Growth and vitality of the trees in vitro and outdoors with and without ectomycorrhizas are reported.

View Article: PubMed Central - PubMed

Affiliation: Forest Botany and Tree Physiology, Büsgen-Institut, Georg-August Universität Göttingen Göttingen, Germany.

ABSTRACT
During the last decades the importance of the genus Populus increased because the poplar genome has been sequenced and molecular tools for basic research have become available. Poplar species occur in different habitats and harbor large genetic variation, which can be exploited for economic applications and for increasing our knowledge on the basic molecular mechanisms of the woody life style. Poplars are, therefore, employed to unravel the molecular mechanisms of wood formation, stress tolerance, tree nutrition and interaction with other organisms such as pathogens or mycorrhiza. The basis of these investigations is the reproducible production of homogeneous plant material. In this method paper we describe techniques and growth conditions for the in vitro propagation of different poplar species (Populus × canescens, P. trichocarpa, P. tremula, and P. euphratica) and ectomycorrhizal fungi (Laccaria bicolor, Paxillus involutus) as well as for their co-cultivation for ectomycorrhizal synthesis. Maintenance and plant preparation require different multiplication and rooting media. Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are described. Transfer of the plants from in vitro to in situ conditions is critical and hardening is important to prevent high mortality. Growth and vitality of the trees in vitro and outdoors with and without ectomycorrhizas are reported.

No MeSH data available.


Related in: MedlinePlus