Limits...
Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH
Fourier-transform infrared (FTIR) spectroscopy of purified bioflocculant produced by the consortium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750929&req=5

Figure 7: Fourier-transform infrared (FTIR) spectroscopy of purified bioflocculant produced by the consortium.

Mentions: Fourier-transform infrared (FTIR) spectrum of the pure bioflocculant showed broad absorption band at 3275 cm-1 and two other sharp bands at 1652 and 1456 (cm-1), which represents the presence of carboxyl and hydroxyl groups from polymeric and dimeric OH stretches of phenol or tertiary alcohol bends [19]. The C-O stretching vibration band at 1011 cm-1 in conjunction with sharp peak at 849 cm-1 indicates the presence of furan saccharides (Figure 7). Similar spectrum has been reported for bioflocculant produced by other microbial species [7,24,25]. These functional groups provide surface charges which serve as the binding sites for suspended particles hence, causing aggregation or floc formation in solutions and/or colloids. The roles of OH-, COO- and H+ groups in the flocculation of suspended particles have been reported for several bioflocculants of microbial origin [26,27].


Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Fourier-transform infrared (FTIR) spectroscopy of purified bioflocculant produced by the consortium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750929&req=5

Figure 7: Fourier-transform infrared (FTIR) spectroscopy of purified bioflocculant produced by the consortium.
Mentions: Fourier-transform infrared (FTIR) spectrum of the pure bioflocculant showed broad absorption band at 3275 cm-1 and two other sharp bands at 1652 and 1456 (cm-1), which represents the presence of carboxyl and hydroxyl groups from polymeric and dimeric OH stretches of phenol or tertiary alcohol bends [19]. The C-O stretching vibration band at 1011 cm-1 in conjunction with sharp peak at 849 cm-1 indicates the presence of furan saccharides (Figure 7). Similar spectrum has been reported for bioflocculant produced by other microbial species [7,24,25]. These functional groups provide surface charges which serve as the binding sites for suspended particles hence, causing aggregation or floc formation in solutions and/or colloids. The roles of OH-, COO- and H+ groups in the flocculation of suspended particles have been reported for several bioflocculants of microbial origin [26,27].

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH