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Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

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ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

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Effect of pH on flocculating activity of purified bioflocculant.
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Figure 5: Effect of pH on flocculating activity of purified bioflocculant.

Mentions: Adjusting the pH of the Kaolin clay suspension from acidic to alkaline (3-12) showed recalcitrance to the effects of H+ concentration. Flocculating activities of more than 60% were observed in the pH range assessed with the highest flocculating activity of 79% obtained at pH 11. Similarly, high flocculation activity (76%) was observed at the acidic pH of 3 (Figure 5). The reason behind the dual flocculation optimum, at an acidic and alkaline pH is unclear. However, the weak acidic pH of 6 was reported as optimum for the bioflocculant produced by the mixed cultures of Staphylococcus and Pseudomonas[13]. Nwodo et al. [18] reported neutral pH as optimally supporting flocculation activity of purified bioflocculant. Various pH has been documented to maximally support flocculation activity of bioflocculants [10,20,23].


Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Effect of pH on flocculating activity of purified bioflocculant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750929&req=5

Figure 5: Effect of pH on flocculating activity of purified bioflocculant.
Mentions: Adjusting the pH of the Kaolin clay suspension from acidic to alkaline (3-12) showed recalcitrance to the effects of H+ concentration. Flocculating activities of more than 60% were observed in the pH range assessed with the highest flocculating activity of 79% obtained at pH 11. Similarly, high flocculation activity (76%) was observed at the acidic pH of 3 (Figure 5). The reason behind the dual flocculation optimum, at an acidic and alkaline pH is unclear. However, the weak acidic pH of 6 was reported as optimum for the bioflocculant produced by the mixed cultures of Staphylococcus and Pseudomonas[13]. Nwodo et al. [18] reported neutral pH as optimally supporting flocculation activity of purified bioflocculant. Various pH has been documented to maximally support flocculation activity of bioflocculants [10,20,23].

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH