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Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

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Stability of the purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium to different medium temperature.
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Figure 4: Stability of the purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium to different medium temperature.

Mentions: Subjecting the bioflocculant to different temperature regimes (50°C, 80°C and 100°C) for a period of 30 min showed thermal stability as flocculation activity was retained. At 80°C, flocculation activity of 86% was obtained (Figure 4). The heat stability of the bioflocculant is remarkable as flocculation activity of more than 70% was retained at 80°C and 100°C of the incubation time. The bioflocculant produced by Rhizobium radiobacter F2 and Bacillus sphaeicus F6 [19] consortium was similarly reported to exhibit thermal stability. Temperature tolerance of polymers is important in deciding suitability for water treatment Patil et al. [4].


Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Stability of the purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium to different medium temperature.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750929&req=5

Figure 4: Stability of the purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium to different medium temperature.
Mentions: Subjecting the bioflocculant to different temperature regimes (50°C, 80°C and 100°C) for a period of 30 min showed thermal stability as flocculation activity was retained. At 80°C, flocculation activity of 86% was obtained (Figure 4). The heat stability of the bioflocculant is remarkable as flocculation activity of more than 70% was retained at 80°C and 100°C of the incubation time. The bioflocculant produced by Rhizobium radiobacter F2 and Bacillus sphaeicus F6 [19] consortium was similarly reported to exhibit thermal stability. Temperature tolerance of polymers is important in deciding suitability for water treatment Patil et al. [4].

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH