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Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

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Optimum flocculating activity determination of purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium following Jar-test.
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Figure 3: Optimum flocculating activity determination of purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium following Jar-test.

Mentions: The evaluation of bioflocculant concentrations, optimal, for flocculation activity revealed 1 mg/ml in 100 ml Kaolin clay suspension (4 g/L) following the Jar test experimentation (Figure 3). At higher concentrations of bioflocculant, flocculation activity declined and this may be explained as inappropriate interaction of the surfaces charges on the bioflocculant in the medium due to saturation effect consequently, less surface area for binding activity ensured. Better still, the counteractive effect of higher bioflocculant concentration, which disturbs the surface charge distribution, may have accounted for the observation. On a similar note, Chan and Chiang, [19] observed that, when flocculants optimum concentration required for flocculation is exceeded; aggregated particles are re-dispersed thus, disallowing particle settling. Nonetheless, optimum concentrations required for flocculation activity are organism dependent; Wang et al.[20] reported 12 mg/l, from the bioflocculant produced by the consortium of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, as the optimum concentration required for the flocculation of Kaolin clay in Jar test experimentation. Likewise, Zheng et al. [21] reported 80 mg/l of bioflocculant produced by Bacillus sp. as optimally causing flocculation of Kaolin clay. Conversely, a lower concentration of 0.3-8.2 (mg/l) with flocculation activity at above 90% [22] was reported for Chryseobacterium daeguese W6.


Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Optimum flocculating activity determination of purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium following Jar-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750929&req=5

Figure 3: Optimum flocculating activity determination of purified bioflocculant produced by Actinobacterium sp. and Methylobacterium sp. consortium following Jar-test.
Mentions: The evaluation of bioflocculant concentrations, optimal, for flocculation activity revealed 1 mg/ml in 100 ml Kaolin clay suspension (4 g/L) following the Jar test experimentation (Figure 3). At higher concentrations of bioflocculant, flocculation activity declined and this may be explained as inappropriate interaction of the surfaces charges on the bioflocculant in the medium due to saturation effect consequently, less surface area for binding activity ensured. Better still, the counteractive effect of higher bioflocculant concentration, which disturbs the surface charge distribution, may have accounted for the observation. On a similar note, Chan and Chiang, [19] observed that, when flocculants optimum concentration required for flocculation is exceeded; aggregated particles are re-dispersed thus, disallowing particle settling. Nonetheless, optimum concentrations required for flocculation activity are organism dependent; Wang et al.[20] reported 12 mg/l, from the bioflocculant produced by the consortium of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, as the optimum concentration required for the flocculation of Kaolin clay in Jar test experimentation. Likewise, Zheng et al. [21] reported 80 mg/l of bioflocculant produced by Bacillus sp. as optimally causing flocculation of Kaolin clay. Conversely, a lower concentration of 0.3-8.2 (mg/l) with flocculation activity at above 90% [22] was reported for Chryseobacterium daeguese W6.

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH