Limits...
Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH
Effect of initial fermentation pH on bioflocculant production by the consortium of Actinobacterium sp. and Methylobacterium sp.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750929&req=5

Figure 2: Effect of initial fermentation pH on bioflocculant production by the consortium of Actinobacterium sp. and Methylobacterium sp.

Mentions: An alkaline pH of 9, at the start of fermentation, optimally supported bioflocculant production with flocculation activity of 89% (Figure 2). At acidic initial fermentation pH, lower flocculation activity was observed. In addition, fermentation time was prolonged yet, comparable flocculation activity with the ambient pH was not achieved. The longer time observed at acidic initial fermentation pH may be interpreted as time needed for the mixed culture to adjust physiologically to the acidic medium. Nonetheless, various initial medium pH has been reported for the production of bioflocculant by different microbial species. Wang et al.[14], reported optimum bioflocculant production by Rhizobium radiobacter F2 and Bacillus sphaeicus F6 at neutral and weak alkaline pH while Nwodo et al. [18] reported weak acidic condition (pH 6.8) as optimally supporting bioflocculant production by Streptomyces sp. Gansen.


Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Effect of initial fermentation pH on bioflocculant production by the consortium of Actinobacterium sp. and Methylobacterium sp.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750929&req=5

Figure 2: Effect of initial fermentation pH on bioflocculant production by the consortium of Actinobacterium sp. and Methylobacterium sp.
Mentions: An alkaline pH of 9, at the start of fermentation, optimally supported bioflocculant production with flocculation activity of 89% (Figure 2). At acidic initial fermentation pH, lower flocculation activity was observed. In addition, fermentation time was prolonged yet, comparable flocculation activity with the ambient pH was not achieved. The longer time observed at acidic initial fermentation pH may be interpreted as time needed for the mixed culture to adjust physiologically to the acidic medium. Nonetheless, various initial medium pH has been reported for the production of bioflocculant by different microbial species. Wang et al.[14], reported optimum bioflocculant production by Rhizobium radiobacter F2 and Bacillus sphaeicus F6 at neutral and weak alkaline pH while Nwodo et al. [18] reported weak acidic condition (pH 6.8) as optimally supporting bioflocculant production by Streptomyces sp. Gansen.

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH