Limits...
Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH
Effect of the inoculum size of Actinobacterium sp. and Methylobacterium sp. consortium on bioflocculant production.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750929&req=5

Figure 1: Effect of the inoculum size of Actinobacterium sp. and Methylobacterium sp. consortium on bioflocculant production.

Mentions: An optimum flocculation activity of 92% was achieved with 1% (v/v) of the mixed culture (Figure 1). Higher inoculum cell densities (1.5% and 2.0%) evaluated, did not yield corresponding increase in flocculating activity. Rather, a slight decrease was observed. This observation is expected as inappropriate ratio of inoculum cell densities to nutrient ratio leads to a phenomenon termed “inoculum effect” consequently, a reduction in the desired effect. Similar trend was observed by Zhang et al.[13] in a study on bioflocculant production by a consortium of Staphylococcus and Pseudomonas species, while Wang et al.[17] obtained maximum flocculating activity for a bioflocculant produced by an axenic culture of Klebsiella mobilis when 5% inoculum size was used.


Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor.

Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI - BMC Biotechnol. (2013)

Effect of the inoculum size of Actinobacterium sp. and Methylobacterium sp. consortium on bioflocculant production.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750929&req=5

Figure 1: Effect of the inoculum size of Actinobacterium sp. and Methylobacterium sp. consortium on bioflocculant production.
Mentions: An optimum flocculation activity of 92% was achieved with 1% (v/v) of the mixed culture (Figure 1). Higher inoculum cell densities (1.5% and 2.0%) evaluated, did not yield corresponding increase in flocculating activity. Rather, a slight decrease was observed. This observation is expected as inappropriate ratio of inoculum cell densities to nutrient ratio leads to a phenomenon termed “inoculum effect” consequently, a reduction in the desired effect. Similar trend was observed by Zhang et al.[13] in a study on bioflocculant production by a consortium of Staphylococcus and Pseudomonas species, while Wang et al.[17] obtained maximum flocculating activity for a bioflocculant produced by an axenic culture of Klebsiella mobilis when 5% inoculum size was used.

Bottom Line: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems.Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.The mixed culture produced bioflocculant with high flocculating activity and an improved yield.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results: 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Conclusions: The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

Show MeSH