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Scanning electron microscopy of lichen sclerosus.

Almeida HL, Bicca Ede B, Breunig Jde A, Rocha NM, Silva RM - An Bras Dermatol (2013 Mar-Apr)

Bottom Line: Normal collagen fibers could be easily found in deeper layers of the specimen, as well as the transition to pathologic area, which seems homogenized.Rupture of collagen fibers and inflammatory cells were not found.These autoimmune changes of the extracellular matrix lead to the aggregation of immune complexes and/or changed matrix proteins along the collagen fibers, the reason why they seem hyalinized when examined by light microscopy.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Pelotas, Pelotas, RS, Brazil. hiramalmeidajr@hotmail.com

ABSTRACT
Lichen sclerosus is an acquired inflammatory condition characterized by whitish fibrotic plaques, with a predilection for the genital skin. We performed scanning electron microscopy of the dermis from a lesion of lichen sclerosus. Normal collagen fibers could be easily found in deeper layers of the specimen, as well as the transition to pathologic area, which seems homogenized. With higher magnifications in this transitional area collagen fibers are adherent to each other, and with very high magnifications a pearl chain aspect became evident along the collagen fibers. In the superficial dermis this homogenization is even more evident, collagen fibers are packed together and round structures are also observed. Rupture of collagen fibers and inflammatory cells were not found. These autoimmune changes of the extracellular matrix lead to the aggregation of immune complexes and/or changed matrix proteins along the collagen fibers, the reason why they seem hyalinized when examined by light microscopy.

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Scanning electron microscopy -(a). homogenized area (x 10,000) (b). detail showingthe pearl chain aspect of the collagen fibers (x 20,000)
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f03: Scanning electron microscopy -(a). homogenized area (x 10,000) (b). detail showingthe pearl chain aspect of the collagen fibers (x 20,000)

Mentions: Normal collagen fibers could be easily found in deeper layers of the fragment, as wellas the transition to the pathologic area, which seems homogenized (Figure 2). With higher magnifications in this transitional areacollagen fibers are adherent to each other, and with very high magnifications (x 20,000)a pearl chain aspect became evident along the collagen fibers (Figure 3). In the superficial dermis this homogenization is evenmore evident; collagen fibers are packed together and round structures are also observed(Figure 4). Rupture of collagen fibers andinflammatory cells were not found.


Scanning electron microscopy of lichen sclerosus.

Almeida HL, Bicca Ede B, Breunig Jde A, Rocha NM, Silva RM - An Bras Dermatol (2013 Mar-Apr)

Scanning electron microscopy -(a). homogenized area (x 10,000) (b). detail showingthe pearl chain aspect of the collagen fibers (x 20,000)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750889&req=5

f03: Scanning electron microscopy -(a). homogenized area (x 10,000) (b). detail showingthe pearl chain aspect of the collagen fibers (x 20,000)
Mentions: Normal collagen fibers could be easily found in deeper layers of the fragment, as wellas the transition to the pathologic area, which seems homogenized (Figure 2). With higher magnifications in this transitional areacollagen fibers are adherent to each other, and with very high magnifications (x 20,000)a pearl chain aspect became evident along the collagen fibers (Figure 3). In the superficial dermis this homogenization is evenmore evident; collagen fibers are packed together and round structures are also observed(Figure 4). Rupture of collagen fibers andinflammatory cells were not found.

Bottom Line: Normal collagen fibers could be easily found in deeper layers of the specimen, as well as the transition to pathologic area, which seems homogenized.Rupture of collagen fibers and inflammatory cells were not found.These autoimmune changes of the extracellular matrix lead to the aggregation of immune complexes and/or changed matrix proteins along the collagen fibers, the reason why they seem hyalinized when examined by light microscopy.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Pelotas, Pelotas, RS, Brazil. hiramalmeidajr@hotmail.com

ABSTRACT
Lichen sclerosus is an acquired inflammatory condition characterized by whitish fibrotic plaques, with a predilection for the genital skin. We performed scanning electron microscopy of the dermis from a lesion of lichen sclerosus. Normal collagen fibers could be easily found in deeper layers of the specimen, as well as the transition to pathologic area, which seems homogenized. With higher magnifications in this transitional area collagen fibers are adherent to each other, and with very high magnifications a pearl chain aspect became evident along the collagen fibers. In the superficial dermis this homogenization is even more evident, collagen fibers are packed together and round structures are also observed. Rupture of collagen fibers and inflammatory cells were not found. These autoimmune changes of the extracellular matrix lead to the aggregation of immune complexes and/or changed matrix proteins along the collagen fibers, the reason why they seem hyalinized when examined by light microscopy.

Show MeSH
Related in: MedlinePlus