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Immunohistochemical detection of the latent nuclear antigen-1 of the human herpesvirus type 8 to differentiate cutaneous epidemic Kaposi sarcoma and its histological simulators.

Pereira PF, Cuzzi T, Galhardo MC - An Bras Dermatol (2013 Mar-Apr)

Bottom Line: Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis.HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident.It also contributes to its histological differential diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Evandro Chagas Clinical Research Institute, Oswaldo Cruz Foundation (IPEC-FIOCRUZ), Rio de Janeiro, Brazil. drapatriciafonsecap@gmail.com

ABSTRACT
Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis. The aim of this study is to confirm that immunohistochemistry is a valuable tool for differentiating KS from its simulators in skin biopsies of HIV patients. Immunohistochemical and histological analyses were performed in 49 Kaposi's sarcoma skin biopsies and 60 of its histological simulators. Positivity was present in the 49 Kaposi's sarcoma skin biopsies and no staining was observed in the 60 simulators analyzed, resulting in sensibility and specificity of 100%. HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident. It also contributes to its histological differential diagnosis.

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Immunohistochemistry - Inicial KS lesion. Positivity for the HHV-8-LNA-1 antigenas observed in endothelium cells is initial KS lesion. (IHQ, 40x)
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f01: Immunohistochemistry - Inicial KS lesion. Positivity for the HHV-8-LNA-1 antigenas observed in endothelium cells is initial KS lesion. (IHQ, 40x)

Mentions: Positive nuclear reaction to HHV-8 was detected in the lesional areas in all cases of KS(Figures 1 and 2). The endothelium cells of the normal vessels were negative. Negativereaction was observed in all of its histological simulators analyzed.Immuno-histochemical positive nuclear reaction was observed in all lymph node KS slidesused as controls.


Immunohistochemical detection of the latent nuclear antigen-1 of the human herpesvirus type 8 to differentiate cutaneous epidemic Kaposi sarcoma and its histological simulators.

Pereira PF, Cuzzi T, Galhardo MC - An Bras Dermatol (2013 Mar-Apr)

Immunohistochemistry - Inicial KS lesion. Positivity for the HHV-8-LNA-1 antigenas observed in endothelium cells is initial KS lesion. (IHQ, 40x)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750888&req=5

f01: Immunohistochemistry - Inicial KS lesion. Positivity for the HHV-8-LNA-1 antigenas observed in endothelium cells is initial KS lesion. (IHQ, 40x)
Mentions: Positive nuclear reaction to HHV-8 was detected in the lesional areas in all cases of KS(Figures 1 and 2). The endothelium cells of the normal vessels were negative. Negativereaction was observed in all of its histological simulators analyzed.Immuno-histochemical positive nuclear reaction was observed in all lymph node KS slidesused as controls.

Bottom Line: Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis.HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident.It also contributes to its histological differential diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Evandro Chagas Clinical Research Institute, Oswaldo Cruz Foundation (IPEC-FIOCRUZ), Rio de Janeiro, Brazil. drapatriciafonsecap@gmail.com

ABSTRACT
Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis. The aim of this study is to confirm that immunohistochemistry is a valuable tool for differentiating KS from its simulators in skin biopsies of HIV patients. Immunohistochemical and histological analyses were performed in 49 Kaposi's sarcoma skin biopsies and 60 of its histological simulators. Positivity was present in the 49 Kaposi's sarcoma skin biopsies and no staining was observed in the 60 simulators analyzed, resulting in sensibility and specificity of 100%. HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident. It also contributes to its histological differential diagnosis.

Show MeSH
Related in: MedlinePlus