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Dietary glutamine supplementation prevents mucosal injury and modulates intestinal epithelial restitution following acetic acid induced intestinal injury in rats.

Swaid F, Sukhotnik I, Matter I, Berkowitz D, Hadjittofi C, Pollak Y, Lavy A - Nutr Metab (Lond) (2013)

Bottom Line: Beneficial effects of glutamine (GLN) have been described in many gastrointestinal disorders.AA-induced intestinal injury resulted in a significantly increased intestinal injury score with concomitant inhibition of cell turnover (reduced proliferation and enhanced apoptosis).Treatment with dietary GLN supplementation resulted in a decreased intestinal injury score with concomitant stimulation of cell turnover (enhanced proliferation and reduced apoptosis).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Surgery, Bnai Zion Medical Center, Haifa, Israel.

ABSTRACT
Beneficial effects of glutamine (GLN) have been described in many gastrointestinal disorders. The aim of the present study was to evaluate the preventative effect of oral GLN supplementation against acetic acid (AA) induced intestinal injury in a rat. Male Sprague-Dawley rats were divided into four experimental groups: control (CONTR) rats underwent laparotomy, control-glutamine (CONTR-GLN) rats were treated with enteral glutamine given in drinking water (2%) 48 hours before and five days following laparotomy, AA rats underwent laparotomy and injection of AA into an isolated jejunal loop, and acetic acid-glutamine (AA-GLN) rats underwent AA-induced injury and were treated with enteral GLN 48 hours before and 5 days following laparotomy. Intestinal mucosal damage (Park's injury score), mucosal structural changes, enterocyte proliferation and enterocyte apoptosis were determined five days following intestinal injury. Western blotting was used to determine p-ERK and bax protein levels. AA-induced intestinal injury resulted in a significantly increased intestinal injury score with concomitant inhibition of cell turnover (reduced proliferation and enhanced apoptosis). Treatment with dietary GLN supplementation resulted in a decreased intestinal injury score with concomitant stimulation of cell turnover (enhanced proliferation and reduced apoptosis). In conclusion, pre-treatment with oral GLN prevents mucosal injury and improves intestinal recovery following AA-induced intestinal injury in rats.

No MeSH data available.


Related in: MedlinePlus

Effect of enteral glutamine on macroscopic intestinal appearance during acetic acid-induced intestinal damage. Values are mean ± SEM. CONTR-control; AA- acetic acid; GLN- glutamine. * P < 0.05 vs CONTR rats, † P < 0.05 AA-GLN vs AA rats.
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Figure 3: Effect of enteral glutamine on macroscopic intestinal appearance during acetic acid-induced intestinal damage. Values are mean ± SEM. CONTR-control; AA- acetic acid; GLN- glutamine. * P < 0.05 vs CONTR rats, † P < 0.05 AA-GLN vs AA rats.

Mentions: Treatment of control animals with enteral glutamine (CONTR-GLN group) did not significantly change bowel and mucosal weight compared to control animals (C group). 5 days after intestinal damage, there was a decrease in intestinal wall thickness and gut diameter. AA rats had a significantly lower bowel weight in ileum (44 ± 7 vs. 54 ± 2, p < 0.05) and mucosal weight in both jejunum (23 ± 4 vs. 28 ± 1, p < 0.05) and ileum (9 ± 3 vs. 22 ± 1, p < 0.05) compared to control animals (C group) (Figure 3). AA-GLN rats demonstrated a trend toward increase in ileal bowel and mucosal weight; however, this trend did not achieve statistical significance.


Dietary glutamine supplementation prevents mucosal injury and modulates intestinal epithelial restitution following acetic acid induced intestinal injury in rats.

Swaid F, Sukhotnik I, Matter I, Berkowitz D, Hadjittofi C, Pollak Y, Lavy A - Nutr Metab (Lond) (2013)

Effect of enteral glutamine on macroscopic intestinal appearance during acetic acid-induced intestinal damage. Values are mean ± SEM. CONTR-control; AA- acetic acid; GLN- glutamine. * P < 0.05 vs CONTR rats, † P < 0.05 AA-GLN vs AA rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750704&req=5

Figure 3: Effect of enteral glutamine on macroscopic intestinal appearance during acetic acid-induced intestinal damage. Values are mean ± SEM. CONTR-control; AA- acetic acid; GLN- glutamine. * P < 0.05 vs CONTR rats, † P < 0.05 AA-GLN vs AA rats.
Mentions: Treatment of control animals with enteral glutamine (CONTR-GLN group) did not significantly change bowel and mucosal weight compared to control animals (C group). 5 days after intestinal damage, there was a decrease in intestinal wall thickness and gut diameter. AA rats had a significantly lower bowel weight in ileum (44 ± 7 vs. 54 ± 2, p < 0.05) and mucosal weight in both jejunum (23 ± 4 vs. 28 ± 1, p < 0.05) and ileum (9 ± 3 vs. 22 ± 1, p < 0.05) compared to control animals (C group) (Figure 3). AA-GLN rats demonstrated a trend toward increase in ileal bowel and mucosal weight; however, this trend did not achieve statistical significance.

Bottom Line: Beneficial effects of glutamine (GLN) have been described in many gastrointestinal disorders.AA-induced intestinal injury resulted in a significantly increased intestinal injury score with concomitant inhibition of cell turnover (reduced proliferation and enhanced apoptosis).Treatment with dietary GLN supplementation resulted in a decreased intestinal injury score with concomitant stimulation of cell turnover (enhanced proliferation and reduced apoptosis).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Surgery, Bnai Zion Medical Center, Haifa, Israel.

ABSTRACT
Beneficial effects of glutamine (GLN) have been described in many gastrointestinal disorders. The aim of the present study was to evaluate the preventative effect of oral GLN supplementation against acetic acid (AA) induced intestinal injury in a rat. Male Sprague-Dawley rats were divided into four experimental groups: control (CONTR) rats underwent laparotomy, control-glutamine (CONTR-GLN) rats were treated with enteral glutamine given in drinking water (2%) 48 hours before and five days following laparotomy, AA rats underwent laparotomy and injection of AA into an isolated jejunal loop, and acetic acid-glutamine (AA-GLN) rats underwent AA-induced injury and were treated with enteral GLN 48 hours before and 5 days following laparotomy. Intestinal mucosal damage (Park's injury score), mucosal structural changes, enterocyte proliferation and enterocyte apoptosis were determined five days following intestinal injury. Western blotting was used to determine p-ERK and bax protein levels. AA-induced intestinal injury resulted in a significantly increased intestinal injury score with concomitant inhibition of cell turnover (reduced proliferation and enhanced apoptosis). Treatment with dietary GLN supplementation resulted in a decreased intestinal injury score with concomitant stimulation of cell turnover (enhanced proliferation and reduced apoptosis). In conclusion, pre-treatment with oral GLN prevents mucosal injury and improves intestinal recovery following AA-induced intestinal injury in rats.

No MeSH data available.


Related in: MedlinePlus