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Diel leaf growth of soybean: a novel method to analyze two-dimensional leaf expansion in high temporal resolution based on a marker tracking approach (Martrack Leaf).

Mielewczik M, Friedli M, Kirchgessner N, Walter A - Plant Methods (2013)

Bottom Line: The beads are mechanically constricted to the focal plane of a camera.Algorithms are provided to the entire community interested in using such approaches.It will be beneficial for the further elucidation of genotype x environment x management interactions affecting leaf growth processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: ETH Zürich, Institute of Agricultural Sciences, Universitätstr, 2, CH-8092 Zürich, Switzerland. norbert.kirchgessner@usys.ethz.ch.

ABSTRACT

Background: We present a novel method for quantitative analysis of dicot leaf expansion at high temporal resolution. Image sequences of growing leaves were assessed using a marker tracking algorithm. An important feature of the method is the attachment of dark beads that serve as artificial landmarks to the leaf margin. The beads are mechanically constricted to the focal plane of a camera. Leaf expansion is approximated by the increase in area of the polygon defined by the centers of mass of the beads surrounding the leaf. Fluctuating illumination conditions often pose serious problems for tracking natural structures of a leaf; this problem is circumvented here by the use of the beads.

Results: The new method has been used to assess leaf growth in environmental situations with different illumination conditions that are typical in agricultural and biological experiments: Constant illumination via fluorescent light tubes in a climate chamber, a mix of natural and artificial illumination in a greenhouse and natural illumination of the situation on typical summer days in the field. Typical features of diel (24h) soybean leaf growth patterns were revealed in all three conditions, thereby demonstrating the general applicability of the method. Algorithms are provided to the entire community interested in using such approaches.

Conclusions: The implementation Martrack Leaf presented here is a robust method to investigate diel leaf growth rhythms both under natural and artificial illumination conditions. It will be beneficial for the further elucidation of genotype x environment x management interactions affecting leaf growth processes.

No MeSH data available.


Related in: MedlinePlus

Diel growth pattern of soybean leaves in the field. (A, B) Relative growth rates (RGR) of two soybean leaves on day 1 and day 2; (C) global radiation (Whm-2); (D) relative humidity (%) and (E) temperature (°C) throughout two days in the field.
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Figure 7: Diel growth pattern of soybean leaves in the field. (A, B) Relative growth rates (RGR) of two soybean leaves on day 1 and day 2; (C) global radiation (Whm-2); (D) relative humidity (%) and (E) temperature (°C) throughout two days in the field.

Mentions: In the field, similar diel leaf growth patterns were obtained (Figure 7). At noon though, plants grown in the field showed a pronounced, transient drop in their RGR. Fixation of leaves in the field worked well, and movement of leaves was prevented by leaf fixation even when relatively strong gusts of wind were present. In a period of dry weather, it was therefore possible to monitor leaf growth on three consecutive days without rain. Since the setup and outdoor computer installation was not waterproof, care was taken to protect it from eventually upcoming rain if necessary (Figure 1B).


Diel leaf growth of soybean: a novel method to analyze two-dimensional leaf expansion in high temporal resolution based on a marker tracking approach (Martrack Leaf).

Mielewczik M, Friedli M, Kirchgessner N, Walter A - Plant Methods (2013)

Diel growth pattern of soybean leaves in the field. (A, B) Relative growth rates (RGR) of two soybean leaves on day 1 and day 2; (C) global radiation (Whm-2); (D) relative humidity (%) and (E) temperature (°C) throughout two days in the field.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750653&req=5

Figure 7: Diel growth pattern of soybean leaves in the field. (A, B) Relative growth rates (RGR) of two soybean leaves on day 1 and day 2; (C) global radiation (Whm-2); (D) relative humidity (%) and (E) temperature (°C) throughout two days in the field.
Mentions: In the field, similar diel leaf growth patterns were obtained (Figure 7). At noon though, plants grown in the field showed a pronounced, transient drop in their RGR. Fixation of leaves in the field worked well, and movement of leaves was prevented by leaf fixation even when relatively strong gusts of wind were present. In a period of dry weather, it was therefore possible to monitor leaf growth on three consecutive days without rain. Since the setup and outdoor computer installation was not waterproof, care was taken to protect it from eventually upcoming rain if necessary (Figure 1B).

Bottom Line: The beads are mechanically constricted to the focal plane of a camera.Algorithms are provided to the entire community interested in using such approaches.It will be beneficial for the further elucidation of genotype x environment x management interactions affecting leaf growth processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: ETH Zürich, Institute of Agricultural Sciences, Universitätstr, 2, CH-8092 Zürich, Switzerland. norbert.kirchgessner@usys.ethz.ch.

ABSTRACT

Background: We present a novel method for quantitative analysis of dicot leaf expansion at high temporal resolution. Image sequences of growing leaves were assessed using a marker tracking algorithm. An important feature of the method is the attachment of dark beads that serve as artificial landmarks to the leaf margin. The beads are mechanically constricted to the focal plane of a camera. Leaf expansion is approximated by the increase in area of the polygon defined by the centers of mass of the beads surrounding the leaf. Fluctuating illumination conditions often pose serious problems for tracking natural structures of a leaf; this problem is circumvented here by the use of the beads.

Results: The new method has been used to assess leaf growth in environmental situations with different illumination conditions that are typical in agricultural and biological experiments: Constant illumination via fluorescent light tubes in a climate chamber, a mix of natural and artificial illumination in a greenhouse and natural illumination of the situation on typical summer days in the field. Typical features of diel (24h) soybean leaf growth patterns were revealed in all three conditions, thereby demonstrating the general applicability of the method. Algorithms are provided to the entire community interested in using such approaches.

Conclusions: The implementation Martrack Leaf presented here is a robust method to investigate diel leaf growth rhythms both under natural and artificial illumination conditions. It will be beneficial for the further elucidation of genotype x environment x management interactions affecting leaf growth processes.

No MeSH data available.


Related in: MedlinePlus