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Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.

Xiyang YB, Wang F, Qian BJ, You L, Lu BT, Zhang W, Quan XZ, Ge WP, Liu S, Zhang LF, Wang TH - BMC Biochem. (2013)

Bottom Line: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling.TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival.However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.

ABSTRACT

Background: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.

Results: Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.

Conclusions: The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.

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Related in: MedlinePlus

Relative expressions of TGF-β2 in different tissues of Tg mice. Figure 3 showed the relative optical density (O.D.) of TGF-β2 protein levels in multiple tissues of Tg mice and that of WT ones (n = 6). Values plotted are means ± SD. * compared with WT, P < 0.05. According to formula of the down-regulated rates of TGF-β2 protein, the average down-regulated rates of the four transgenic lines were calculated and described as followed. The average rates were 52%, 25%, 13% and 2% in Founder 66, Founder 16, Founder 53 and Founder 41, respectively. The relative expressions of TGF-β1 proteins in Founder 66, Founder 16 and Founder 53 were significantly different compared with that of WT mice (P < 0.05).
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Figure 3: Relative expressions of TGF-β2 in different tissues of Tg mice. Figure 3 showed the relative optical density (O.D.) of TGF-β2 protein levels in multiple tissues of Tg mice and that of WT ones (n = 6). Values plotted are means ± SD. * compared with WT, P < 0.05. According to formula of the down-regulated rates of TGF-β2 protein, the average down-regulated rates of the four transgenic lines were calculated and described as followed. The average rates were 52%, 25%, 13% and 2% in Founder 66, Founder 16, Founder 53 and Founder 41, respectively. The relative expressions of TGF-β1 proteins in Founder 66, Founder 16 and Founder 53 were significantly different compared with that of WT mice (P < 0.05).

Mentions: Results of Western blot, which detected in different multiple tissues of four genotypes TG (Founder 66, Founder 16, Founder 53 and Founder 41), indicated that TGF-β2 expressions were down-regulated by different percentages in the four kinds of TG mice (Figures 2 and 3). The rates of protein down-regulation were calculated as following: Rates of protein down-regulation = O.D. of WT- O.D. of Founder/O.D. of WT *100%. (O.D.: optical density).


Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.

Xiyang YB, Wang F, Qian BJ, You L, Lu BT, Zhang W, Quan XZ, Ge WP, Liu S, Zhang LF, Wang TH - BMC Biochem. (2013)

Relative expressions of TGF-β2 in different tissues of Tg mice. Figure 3 showed the relative optical density (O.D.) of TGF-β2 protein levels in multiple tissues of Tg mice and that of WT ones (n = 6). Values plotted are means ± SD. * compared with WT, P < 0.05. According to formula of the down-regulated rates of TGF-β2 protein, the average down-regulated rates of the four transgenic lines were calculated and described as followed. The average rates were 52%, 25%, 13% and 2% in Founder 66, Founder 16, Founder 53 and Founder 41, respectively. The relative expressions of TGF-β1 proteins in Founder 66, Founder 16 and Founder 53 were significantly different compared with that of WT mice (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750643&req=5

Figure 3: Relative expressions of TGF-β2 in different tissues of Tg mice. Figure 3 showed the relative optical density (O.D.) of TGF-β2 protein levels in multiple tissues of Tg mice and that of WT ones (n = 6). Values plotted are means ± SD. * compared with WT, P < 0.05. According to formula of the down-regulated rates of TGF-β2 protein, the average down-regulated rates of the four transgenic lines were calculated and described as followed. The average rates were 52%, 25%, 13% and 2% in Founder 66, Founder 16, Founder 53 and Founder 41, respectively. The relative expressions of TGF-β1 proteins in Founder 66, Founder 16 and Founder 53 were significantly different compared with that of WT mice (P < 0.05).
Mentions: Results of Western blot, which detected in different multiple tissues of four genotypes TG (Founder 66, Founder 16, Founder 53 and Founder 41), indicated that TGF-β2 expressions were down-regulated by different percentages in the four kinds of TG mice (Figures 2 and 3). The rates of protein down-regulation were calculated as following: Rates of protein down-regulation = O.D. of WT- O.D. of Founder/O.D. of WT *100%. (O.D.: optical density).

Bottom Line: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling.TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival.However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.

ABSTRACT

Background: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.

Results: Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.

Conclusions: The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.

Show MeSH
Related in: MedlinePlus