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Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.

Xiyang YB, Wang F, Qian BJ, You L, Lu BT, Zhang W, Quan XZ, Ge WP, Liu S, Zhang LF, Wang TH - BMC Biochem. (2013)

Bottom Line: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling.TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival.However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.

ABSTRACT

Background: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.

Results: Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.

Conclusions: The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.

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Protein expressions of TGF-β2 detected by WB in different tissues. Figure 2 Lane 1–5, TGF-β2 protein expression; Lane 1, WT; Lane 2, Founder 66; Lane 3, Founder 16; Lane 4, Founder 53; Lane 5, Founder 41. A, a: olfactory bulb; B, b: cortex; C, c: frontal lobe; D, d: basal forebrain; E, e: cerebellum; F, f: hypothalamus; G, g: medulla oblongata; H, h: spinal cord; I, i: trachea; J, j: lung; K, k: heart; L, l: liver; M, m: spleen; N, n: kidney; O, o: adrenal gland; P, p: intestines; Q, q: skeletal muscles; R, r: epidermis. Beta-tubulin was chased as the control.
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Figure 2: Protein expressions of TGF-β2 detected by WB in different tissues. Figure 2 Lane 1–5, TGF-β2 protein expression; Lane 1, WT; Lane 2, Founder 66; Lane 3, Founder 16; Lane 4, Founder 53; Lane 5, Founder 41. A, a: olfactory bulb; B, b: cortex; C, c: frontal lobe; D, d: basal forebrain; E, e: cerebellum; F, f: hypothalamus; G, g: medulla oblongata; H, h: spinal cord; I, i: trachea; J, j: lung; K, k: heart; L, l: liver; M, m: spleen; N, n: kidney; O, o: adrenal gland; P, p: intestines; Q, q: skeletal muscles; R, r: epidermis. Beta-tubulin was chased as the control.

Mentions: Results of Western blot, which detected in different multiple tissues of four genotypes TG (Founder 66, Founder 16, Founder 53 and Founder 41), indicated that TGF-β2 expressions were down-regulated by different percentages in the four kinds of TG mice (Figures 2 and 3). The rates of protein down-regulation were calculated as following: Rates of protein down-regulation = O.D. of WT- O.D. of Founder/O.D. of WT *100%. (O.D.: optical density).


Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.

Xiyang YB, Wang F, Qian BJ, You L, Lu BT, Zhang W, Quan XZ, Ge WP, Liu S, Zhang LF, Wang TH - BMC Biochem. (2013)

Protein expressions of TGF-β2 detected by WB in different tissues. Figure 2 Lane 1–5, TGF-β2 protein expression; Lane 1, WT; Lane 2, Founder 66; Lane 3, Founder 16; Lane 4, Founder 53; Lane 5, Founder 41. A, a: olfactory bulb; B, b: cortex; C, c: frontal lobe; D, d: basal forebrain; E, e: cerebellum; F, f: hypothalamus; G, g: medulla oblongata; H, h: spinal cord; I, i: trachea; J, j: lung; K, k: heart; L, l: liver; M, m: spleen; N, n: kidney; O, o: adrenal gland; P, p: intestines; Q, q: skeletal muscles; R, r: epidermis. Beta-tubulin was chased as the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750643&req=5

Figure 2: Protein expressions of TGF-β2 detected by WB in different tissues. Figure 2 Lane 1–5, TGF-β2 protein expression; Lane 1, WT; Lane 2, Founder 66; Lane 3, Founder 16; Lane 4, Founder 53; Lane 5, Founder 41. A, a: olfactory bulb; B, b: cortex; C, c: frontal lobe; D, d: basal forebrain; E, e: cerebellum; F, f: hypothalamus; G, g: medulla oblongata; H, h: spinal cord; I, i: trachea; J, j: lung; K, k: heart; L, l: liver; M, m: spleen; N, n: kidney; O, o: adrenal gland; P, p: intestines; Q, q: skeletal muscles; R, r: epidermis. Beta-tubulin was chased as the control.
Mentions: Results of Western blot, which detected in different multiple tissues of four genotypes TG (Founder 66, Founder 16, Founder 53 and Founder 41), indicated that TGF-β2 expressions were down-regulated by different percentages in the four kinds of TG mice (Figures 2 and 3). The rates of protein down-regulation were calculated as following: Rates of protein down-regulation = O.D. of WT- O.D. of Founder/O.D. of WT *100%. (O.D.: optical density).

Bottom Line: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling.TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival.However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.

ABSTRACT

Background: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.

Results: Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.

Conclusions: The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.

Show MeSH
Related in: MedlinePlus