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Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.

Xiyang YB, Wang F, Qian BJ, You L, Lu BT, Zhang W, Quan XZ, Ge WP, Liu S, Zhang LF, Wang TH - BMC Biochem. (2013)

Bottom Line: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling.TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival.However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.

ABSTRACT

Background: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.

Results: Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.

Conclusions: The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.

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Related in: MedlinePlus

Genotypes detection for the TGF-β2-kd Tg mice. The positive Tg mice detected by PCR. Figure 1 showed the representative lanes of products electrophoresed in 1% agarose gel stained with EB. Lane 1: DNA Marker DL 2,000 (from up to down: 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp respectively). Lane 2–9: The PCR productions of inserted fragment from different heterozygous transgenic offspring of TGF-β2-kd lines. Lane 2, Lane 3, Lane 6 and Lane 7: WT; Lane 4: Founder 66; Lane 5: Founder 16; Lane 8: Founder 53; Lane 9: Founder 41.
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Figure 1: Genotypes detection for the TGF-β2-kd Tg mice. The positive Tg mice detected by PCR. Figure 1 showed the representative lanes of products electrophoresed in 1% agarose gel stained with EB. Lane 1: DNA Marker DL 2,000 (from up to down: 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp respectively). Lane 2–9: The PCR productions of inserted fragment from different heterozygous transgenic offspring of TGF-β2-kd lines. Lane 2, Lane 3, Lane 6 and Lane 7: WT; Lane 4: Founder 66; Lane 5: Founder 16; Lane 8: Founder 53; Lane 9: Founder 41.

Mentions: Five heterozygosis transgenic offspring of TGF-β2-kd lines were obtained. Four of them could generate offspring, which were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The Tg mice with inserted fragment, identified by PCR, were regarded as positive Tg (Figure 1).


Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.

Xiyang YB, Wang F, Qian BJ, You L, Lu BT, Zhang W, Quan XZ, Ge WP, Liu S, Zhang LF, Wang TH - BMC Biochem. (2013)

Genotypes detection for the TGF-β2-kd Tg mice. The positive Tg mice detected by PCR. Figure 1 showed the representative lanes of products electrophoresed in 1% agarose gel stained with EB. Lane 1: DNA Marker DL 2,000 (from up to down: 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp respectively). Lane 2–9: The PCR productions of inserted fragment from different heterozygous transgenic offspring of TGF-β2-kd lines. Lane 2, Lane 3, Lane 6 and Lane 7: WT; Lane 4: Founder 66; Lane 5: Founder 16; Lane 8: Founder 53; Lane 9: Founder 41.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750643&req=5

Figure 1: Genotypes detection for the TGF-β2-kd Tg mice. The positive Tg mice detected by PCR. Figure 1 showed the representative lanes of products electrophoresed in 1% agarose gel stained with EB. Lane 1: DNA Marker DL 2,000 (from up to down: 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp respectively). Lane 2–9: The PCR productions of inserted fragment from different heterozygous transgenic offspring of TGF-β2-kd lines. Lane 2, Lane 3, Lane 6 and Lane 7: WT; Lane 4: Founder 66; Lane 5: Founder 16; Lane 8: Founder 53; Lane 9: Founder 41.
Mentions: Five heterozygosis transgenic offspring of TGF-β2-kd lines were obtained. Four of them could generate offspring, which were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The Tg mice with inserted fragment, identified by PCR, were regarded as positive Tg (Figure 1).

Bottom Line: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling.TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival.However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.

ABSTRACT

Background: Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.

Results: Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.

Conclusions: The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.

Show MeSH
Related in: MedlinePlus