Limits...
Flow cytometric analysis of the CD4+ TCR Vβ repertoire in the peripheral blood of children with type 1 diabetes mellitus, systemic lupus erythematosus and age-matched healthy controls.

Tzifi F, Kanariou M, Tzanoudaki M, Mihas C, Paschali E, Chrousos G, Kanaka-Gantenbein C - BMC Immunol. (2013)

Bottom Line: Data regarding the quantitative expression of TCR Vβ subpopulations in children with autoimmune diseases provided interesting and sometimes conflicting results.Statistical analysis revealed that the CD4 + Vβ4 chain was significantly increased in patients with T1DM (p < 0.001), whereas CD4 + Vβ16 one was significantly increased in SLE patients (p < 0.001) compared to controls.CD4 + Vβ4 and CD4 + Vβ16 chains could be possibly involved in the cascade of events precipitating the pathogenesis of T1DM and SLE in children, respectively.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Data regarding the quantitative expression of TCR Vβ subpopulations in children with autoimmune diseases provided interesting and sometimes conflicting results. The aim of the present study was to assess by comparative flow cytometric analysis the peripheral blood CD4+ TCR Vβ repertoire of children with an organ-specific autoimmune disorder, such as type 1 diabetes mellitus (T1DM), in comparison to children with a systemic autoimmune disease, such as Systemic Lupus Erythematosus (SLE) in comparison to healthy age-matched controls of the same ethnic origin. The CD4+ TCR Vβ repertoire was analysed by flow cytometry in three groups of participants: a) fifteen newly diagnosed children with T1DM (mean age: 9.2 ± 4.78 years old), b) nine newly diagnosed children with SLE, positive for ANA and anti-dsDNA, prior to treatment (mean age: 12.8 ±1.76 years old) and c) 31 healthy age-matched controls (mean age: 6.58 ± 3.65 years old), all of Hellenic origin.

Results: CD4 + TCR Vβ abnormalities (± 3SD of controls) were observed mainly in SLE patients. Statistical analysis revealed that the CD4 + Vβ4 chain was significantly increased in patients with T1DM (p < 0.001), whereas CD4 + Vβ16 one was significantly increased in SLE patients (p < 0.001) compared to controls.

Conclusions: CD4 + Vβ4 and CD4 + Vβ16 chains could be possibly involved in the cascade of events precipitating the pathogenesis of T1DM and SLE in children, respectively.

Show MeSH

Related in: MedlinePlus

CD4 + TCR Vβ chains quantitative expression in healthy children. CD4 + TCR Vβ repertoire usage (values %) in healthy controls revealed that increased usage of Vβ subpopulations can be observed sporadically even in healthy children.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750582&req=5

Figure 1: CD4 + TCR Vβ chains quantitative expression in healthy children. CD4 + TCR Vβ repertoire usage (values %) in healthy controls revealed that increased usage of Vβ subpopulations can be observed sporadically even in healthy children.

Mentions: The increased/decreased usage of a Vβ subfamily has arbitrarily been defined as mean value ± 2 or 3 standard deviations (mean value is the one derived from the control sample of the present study) [10,11]. Of the children tested, 16 individuals had increased usage (expressed as mean value + 2 or 3SD) of one or more Vβ lymphocyte populations and these findings concerned different Vβ chains in each subject. Seven children had an increased usage of mean value + 2SD and nine an increased usage of mean value + 3SD. Of these 16 subjects, 11 controls presented with an increased usage in only one chain (Vβ1, Vβ3, Vβ5.1, Vβ5.2, Vβ5.3, Vβ7.1, Vβ12, Vβ14, Vβ16, Vβ20, Vβ22), 4 controls had increased usage in two Vβ subpopulations (Vβ4-Vβ2, Vβ5.1-Vβ21.3, Vβ13.1-Vβ13.6, Vβ7.2-Vβ13.2 ) and one control in five ones (Vβ5.2, Vβ7.4, Vβ8, Vβ9, Vβ18) (Figure 1). No discrepancies were noticed in the Vβ11 and Vβ17 chains.


Flow cytometric analysis of the CD4+ TCR Vβ repertoire in the peripheral blood of children with type 1 diabetes mellitus, systemic lupus erythematosus and age-matched healthy controls.

Tzifi F, Kanariou M, Tzanoudaki M, Mihas C, Paschali E, Chrousos G, Kanaka-Gantenbein C - BMC Immunol. (2013)

CD4 + TCR Vβ chains quantitative expression in healthy children. CD4 + TCR Vβ repertoire usage (values %) in healthy controls revealed that increased usage of Vβ subpopulations can be observed sporadically even in healthy children.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750582&req=5

Figure 1: CD4 + TCR Vβ chains quantitative expression in healthy children. CD4 + TCR Vβ repertoire usage (values %) in healthy controls revealed that increased usage of Vβ subpopulations can be observed sporadically even in healthy children.
Mentions: The increased/decreased usage of a Vβ subfamily has arbitrarily been defined as mean value ± 2 or 3 standard deviations (mean value is the one derived from the control sample of the present study) [10,11]. Of the children tested, 16 individuals had increased usage (expressed as mean value + 2 or 3SD) of one or more Vβ lymphocyte populations and these findings concerned different Vβ chains in each subject. Seven children had an increased usage of mean value + 2SD and nine an increased usage of mean value + 3SD. Of these 16 subjects, 11 controls presented with an increased usage in only one chain (Vβ1, Vβ3, Vβ5.1, Vβ5.2, Vβ5.3, Vβ7.1, Vβ12, Vβ14, Vβ16, Vβ20, Vβ22), 4 controls had increased usage in two Vβ subpopulations (Vβ4-Vβ2, Vβ5.1-Vβ21.3, Vβ13.1-Vβ13.6, Vβ7.2-Vβ13.2 ) and one control in five ones (Vβ5.2, Vβ7.4, Vβ8, Vβ9, Vβ18) (Figure 1). No discrepancies were noticed in the Vβ11 and Vβ17 chains.

Bottom Line: Data regarding the quantitative expression of TCR Vβ subpopulations in children with autoimmune diseases provided interesting and sometimes conflicting results.Statistical analysis revealed that the CD4 + Vβ4 chain was significantly increased in patients with T1DM (p < 0.001), whereas CD4 + Vβ16 one was significantly increased in SLE patients (p < 0.001) compared to controls.CD4 + Vβ4 and CD4 + Vβ16 chains could be possibly involved in the cascade of events precipitating the pathogenesis of T1DM and SLE in children, respectively.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Data regarding the quantitative expression of TCR Vβ subpopulations in children with autoimmune diseases provided interesting and sometimes conflicting results. The aim of the present study was to assess by comparative flow cytometric analysis the peripheral blood CD4+ TCR Vβ repertoire of children with an organ-specific autoimmune disorder, such as type 1 diabetes mellitus (T1DM), in comparison to children with a systemic autoimmune disease, such as Systemic Lupus Erythematosus (SLE) in comparison to healthy age-matched controls of the same ethnic origin. The CD4+ TCR Vβ repertoire was analysed by flow cytometry in three groups of participants: a) fifteen newly diagnosed children with T1DM (mean age: 9.2 ± 4.78 years old), b) nine newly diagnosed children with SLE, positive for ANA and anti-dsDNA, prior to treatment (mean age: 12.8 ±1.76 years old) and c) 31 healthy age-matched controls (mean age: 6.58 ± 3.65 years old), all of Hellenic origin.

Results: CD4 + TCR Vβ abnormalities (± 3SD of controls) were observed mainly in SLE patients. Statistical analysis revealed that the CD4 + Vβ4 chain was significantly increased in patients with T1DM (p < 0.001), whereas CD4 + Vβ16 one was significantly increased in SLE patients (p < 0.001) compared to controls.

Conclusions: CD4 + Vβ4 and CD4 + Vβ16 chains could be possibly involved in the cascade of events precipitating the pathogenesis of T1DM and SLE in children, respectively.

Show MeSH
Related in: MedlinePlus