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Local and systemic neutrophilic inflammation in patients with lung cancer and chronic obstructive pulmonary disease.

Vaguliene N, Zemaitis M, Lavinskiene S, Miliauskas S, Sakalauskas R - BMC Immunol. (2013)

Bottom Line: NE and MPO levels in the serum and BAL fluid were determined by ELISA.ROS production was analyzed by flow cytometer.However, BAL fluid and serum levels of both NE and MPO were significantly higher in patients with lung cancer than COPD patients or healthy individuals (P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pulmonology and Immunology, Medical Academy, Hospital of Lithuanian University of Health Sciences, Eiveniu 2, Kaunas LT-50028, Lithuania. neringa.vaguliene@gmail.com

ABSTRACT

Background: Recent investigations suggest that neutrophils play an important role in the immune response to lung cancer as well as chronic obstructive pulmonary disease (COPD). The aim of this study was to evaluate the amount of neutrophils and markers of their activity in lung cancer and COPD and in coexistence of these two diseases.

Methods: In total, 267 persons were included in the study: 139 patients with lung cancer, 55 patients with lung cancer and COPD, 40 patients with COPD, and 33 healthy subjects. Peripheral blood and BAL fluid samples were obtained for cell count analysis and determination of NE, MPO levels and ROS production. NE and MPO levels in the serum and BAL fluid were determined by ELISA. ROS production was analyzed by flow cytometer.

Results: The percentage, cell count of neutrophils and neutrophil to lymphocyte ratio in the peripheral blood were significantly higher in lung cancer patients with or without COPD compared to COPD patients or healthy individuals (P < 0.05). The percentage and cell count of neutrophils in BAL fluid were significantly lower in patients with lung cancer with or without COPD than in patients with COPD (P < 0.05). However, BAL fluid and serum levels of both NE and MPO were significantly higher in patients with lung cancer than COPD patients or healthy individuals (P < 0.05). Neutrophils produced higher amounts of ROS in patients with lung cancer with or without COPD compared with COPD patients or healthy individuals (P < 0.05).

Conclusions: The results from this study demonstrate higher degree of local and systemic neutrophilic inflammation in patients with lung cancer (with or without COPD) than in patients with COPD.

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Related in: MedlinePlus

NE and MPO levels (ng/mL) in serum and BAL fluid in patients with advanced and early stage lung cancer, patients with lung cancer/COPD, patients with COPD and healthy individuals. Data are presented as mean ± SEM. *P < 0.05, when compared with COPD and healthy individuals; §P < 0.05, when compared with healthy individuals.
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Figure 2: NE and MPO levels (ng/mL) in serum and BAL fluid in patients with advanced and early stage lung cancer, patients with lung cancer/COPD, patients with COPD and healthy individuals. Data are presented as mean ± SEM. *P < 0.05, when compared with COPD and healthy individuals; §P < 0.05, when compared with healthy individuals.

Mentions: Serum and BAL fluid levels of both NE and MPO were significantly higher in patients with lung cancer than in patients with COPD or healthy individuals (P < 0.05) (Figure 2). However, serum and BAL fluid NE and MPO levels did not significantly differ in lung cancer groups (with and without COPD) (P > 0.05). We did not find any significant differences of NE and MPO levels in serum and BAL between the male and female patients with lung cancer (NE serum 518.64 ± 10.38 ng/mL vs. 489.22 ± 15.17 ng/mL, NE BAL fluid 297.34 ± 9.88 ng/mL vs. 327.77 ± 18.79 ng/mL, MPO serum 297.56 ± 3.22 ng/mL vs. 301.54 ± 15.80 ng/mL, MPO BAL fluid 111.69 ± 13.64 ng/mL vs. 112.83 ± 10.76 ng/mL, P > 0.05). There were no significant differences of NE and MPO levels in serum and BAL fluid between the non-small cell lung cancer and small cell lung cancer (NE serum 512.19 ± 8.99 ng/mL vs. 537.72 ± 11.97 ng/mL, NE BAL fluid 316.53 ± 8.43 ng/mL vs. 332.65 ± 25.03 ng/mL, MPO serum 298.35 ± 13.16 ng/mL vs. 305.31 ± 17.32 ng/mL, MPO BAL fluid 114.09 ± 14.08 ng/mL vs. 130.83 ± 10.91 ng/mL, P > 0.05) as well as among various histological types of cancer (data not shown). However, serum NE and MPO levels were significantly higher in patients with advanced lung cancer than in those with early lung cancer (P < 0.05) (Figure 2). Furthermore, patients with early lung cancer had a significantly higher NE levels than patients with COPD (P < 0.01).There were no significant differences of NE and MPO levels in serum and BAL fluid in lung cancer patients with performance status 0–1 when comparing to patients with performance status 2–3 (NE serum 509.51 ± 8.76 ng/mL vs. 528.01 ± 18.45 ng/mL, NE BAL fluid 314.38 ± 8.29 ng/mL vs. 338.21 ± 25.08 ng/mL, MPO serum 297.13 ± 13.00 ng/mL vs. 300.85 ± 18.03 ng/mL, MPO BAL fluid 112.94 ± 14.14 ng/mL vs. 121.72 ± 11.92 ng/mL, P > 0.05). There were no differences of NE and MPO levels in lung cancer groups (with or without COPD) among subjects that have never smoked, former and current smokers (P > 0.05). Additionally, NE and MPO levels in serum and BAL fluid were found to be significantly higher in the lung cancer patients, who have never smoked compared with the current smokers with COPD (NE serum 480.11 ± 19.05 vs. 132.51 ± 18.72, MPO serum 286.96 ± 9.94 vs. 183.42 ± 11.95, MPO BAL fluid 103.65 ± 5.19 vs. 68.43 ± 7.59, P < 0.05). Correlations between NE, MPO levels and spontaneous ROS production in the peripheral blood neutrophils in patients with lung cancer are presented in Table 4. No correlations were found between serum and BAL fluid NE, MPO levels and age, BMI, smoking intensity in the investigated groups (data not shown).


Local and systemic neutrophilic inflammation in patients with lung cancer and chronic obstructive pulmonary disease.

Vaguliene N, Zemaitis M, Lavinskiene S, Miliauskas S, Sakalauskas R - BMC Immunol. (2013)

NE and MPO levels (ng/mL) in serum and BAL fluid in patients with advanced and early stage lung cancer, patients with lung cancer/COPD, patients with COPD and healthy individuals. Data are presented as mean ± SEM. *P < 0.05, when compared with COPD and healthy individuals; §P < 0.05, when compared with healthy individuals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750549&req=5

Figure 2: NE and MPO levels (ng/mL) in serum and BAL fluid in patients with advanced and early stage lung cancer, patients with lung cancer/COPD, patients with COPD and healthy individuals. Data are presented as mean ± SEM. *P < 0.05, when compared with COPD and healthy individuals; §P < 0.05, when compared with healthy individuals.
Mentions: Serum and BAL fluid levels of both NE and MPO were significantly higher in patients with lung cancer than in patients with COPD or healthy individuals (P < 0.05) (Figure 2). However, serum and BAL fluid NE and MPO levels did not significantly differ in lung cancer groups (with and without COPD) (P > 0.05). We did not find any significant differences of NE and MPO levels in serum and BAL between the male and female patients with lung cancer (NE serum 518.64 ± 10.38 ng/mL vs. 489.22 ± 15.17 ng/mL, NE BAL fluid 297.34 ± 9.88 ng/mL vs. 327.77 ± 18.79 ng/mL, MPO serum 297.56 ± 3.22 ng/mL vs. 301.54 ± 15.80 ng/mL, MPO BAL fluid 111.69 ± 13.64 ng/mL vs. 112.83 ± 10.76 ng/mL, P > 0.05). There were no significant differences of NE and MPO levels in serum and BAL fluid between the non-small cell lung cancer and small cell lung cancer (NE serum 512.19 ± 8.99 ng/mL vs. 537.72 ± 11.97 ng/mL, NE BAL fluid 316.53 ± 8.43 ng/mL vs. 332.65 ± 25.03 ng/mL, MPO serum 298.35 ± 13.16 ng/mL vs. 305.31 ± 17.32 ng/mL, MPO BAL fluid 114.09 ± 14.08 ng/mL vs. 130.83 ± 10.91 ng/mL, P > 0.05) as well as among various histological types of cancer (data not shown). However, serum NE and MPO levels were significantly higher in patients with advanced lung cancer than in those with early lung cancer (P < 0.05) (Figure 2). Furthermore, patients with early lung cancer had a significantly higher NE levels than patients with COPD (P < 0.01).There were no significant differences of NE and MPO levels in serum and BAL fluid in lung cancer patients with performance status 0–1 when comparing to patients with performance status 2–3 (NE serum 509.51 ± 8.76 ng/mL vs. 528.01 ± 18.45 ng/mL, NE BAL fluid 314.38 ± 8.29 ng/mL vs. 338.21 ± 25.08 ng/mL, MPO serum 297.13 ± 13.00 ng/mL vs. 300.85 ± 18.03 ng/mL, MPO BAL fluid 112.94 ± 14.14 ng/mL vs. 121.72 ± 11.92 ng/mL, P > 0.05). There were no differences of NE and MPO levels in lung cancer groups (with or without COPD) among subjects that have never smoked, former and current smokers (P > 0.05). Additionally, NE and MPO levels in serum and BAL fluid were found to be significantly higher in the lung cancer patients, who have never smoked compared with the current smokers with COPD (NE serum 480.11 ± 19.05 vs. 132.51 ± 18.72, MPO serum 286.96 ± 9.94 vs. 183.42 ± 11.95, MPO BAL fluid 103.65 ± 5.19 vs. 68.43 ± 7.59, P < 0.05). Correlations between NE, MPO levels and spontaneous ROS production in the peripheral blood neutrophils in patients with lung cancer are presented in Table 4. No correlations were found between serum and BAL fluid NE, MPO levels and age, BMI, smoking intensity in the investigated groups (data not shown).

Bottom Line: NE and MPO levels in the serum and BAL fluid were determined by ELISA.ROS production was analyzed by flow cytometer.However, BAL fluid and serum levels of both NE and MPO were significantly higher in patients with lung cancer than COPD patients or healthy individuals (P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pulmonology and Immunology, Medical Academy, Hospital of Lithuanian University of Health Sciences, Eiveniu 2, Kaunas LT-50028, Lithuania. neringa.vaguliene@gmail.com

ABSTRACT

Background: Recent investigations suggest that neutrophils play an important role in the immune response to lung cancer as well as chronic obstructive pulmonary disease (COPD). The aim of this study was to evaluate the amount of neutrophils and markers of their activity in lung cancer and COPD and in coexistence of these two diseases.

Methods: In total, 267 persons were included in the study: 139 patients with lung cancer, 55 patients with lung cancer and COPD, 40 patients with COPD, and 33 healthy subjects. Peripheral blood and BAL fluid samples were obtained for cell count analysis and determination of NE, MPO levels and ROS production. NE and MPO levels in the serum and BAL fluid were determined by ELISA. ROS production was analyzed by flow cytometer.

Results: The percentage, cell count of neutrophils and neutrophil to lymphocyte ratio in the peripheral blood were significantly higher in lung cancer patients with or without COPD compared to COPD patients or healthy individuals (P < 0.05). The percentage and cell count of neutrophils in BAL fluid were significantly lower in patients with lung cancer with or without COPD than in patients with COPD (P < 0.05). However, BAL fluid and serum levels of both NE and MPO were significantly higher in patients with lung cancer than COPD patients or healthy individuals (P < 0.05). Neutrophils produced higher amounts of ROS in patients with lung cancer with or without COPD compared with COPD patients or healthy individuals (P < 0.05).

Conclusions: The results from this study demonstrate higher degree of local and systemic neutrophilic inflammation in patients with lung cancer (with or without COPD) than in patients with COPD.

Show MeSH
Related in: MedlinePlus