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Mixed-function oxidases and esterases associated with permethrin, deltamethrin and bendiocarb resistance in Anopheles gambiae s.l. in the south-north transect Benin, West Africa.

Aïzoun N, Aïkpon R, Padonou GG, Oussou O, Oké-Agbo F, Gnanguenon V, Ossè R, Akogbéto M - Parasit Vectors (2013)

Bottom Line: The susceptibility of Anopheles gambiae Agbalilame and Kandi populations to permethrin and deltamethrin respectively, increased significantly when synergized by PBO, suggesting an implication of mono-oxygenases in resistance of Anopheles gambiae s.l. to pyrethroid.Esterases may play a role in bendiocarb resistance in Anopheles gambiae Tanguieta.Synergists partially restored susceptibility to pyrethroid and carbamate insecticides and might help mitigate the impact of vector resistance in Anopheles gambiae Agbalilame, Kandi and Tanguieta populations.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Insecticide resistance monitoring is essential to help national programmers to implement more effective and sustainable malaria control strategies in endemic countries. The current study aimed at an exploring the involvement of detoxifying enzymes in the insecticide phenotype resistance in Anopheles gambiae s.l.from Benin, in order to guide future malaria vector control interventions.

Methods: Larvae and pupae of Anopheles gambiae s.l. mosquitoes were collected from the breeding sites in Oueme, Atacora and Alibori provinces. CDC susceptibility tests were conducted on unfed female mosquitoes aged 2-5 days old. CDC bioassays were performed with stock solutions of permethrin (21.5 μg per bottle), deltamethrin (12.5 μg per bottle) and bendiocarb (12.5 μg per bottle). CDC biochemical assays using synergists were also conducted to assess the metabolic resistance.

Results: The susceptibility of Anopheles gambiae Agbalilame and Kandi populations to permethrin and deltamethrin respectively, increased significantly when synergized by PBO, suggesting an implication of mono-oxygenases in resistance of Anopheles gambiae s.l. to pyrethroid. Esterases may play a role in bendiocarb resistance in Anopheles gambiae Tanguieta.

Conclusion: Synergists partially restored susceptibility to pyrethroid and carbamate insecticides and might help mitigate the impact of vector resistance in Anopheles gambiae Agbalilame, Kandi and Tanguieta populations. However, additional vector control tools are needed to further impact on malaria transmission in such settings.This will improve the implementation and management of future control programs against this important malaria vector in Benin and in Africa in general.

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Map of the study area.
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Figure 1: Map of the study area.

Mentions: The study was carried out in the South of Benin, more precisely in Agbalilame, in the Seme district of Oueme province and in the North of Benin, in the Tanguieta district of Atacora province and in the Kandi district of Alibori province (Figure 1). The choice of the study sites took into account the economic activities of populations, their usual protection practices against mosquito bites, the indoor residual spraying (IRS) in progress in certain of these localities and peasant practices to control farming pests. These factors have an impact on the resistance development in the local vector mosquitoes. Oueme has a climate with four seasons, two rainy seasons (March-July and September-November) and two dry seasons (December-March and August-September).The temperature ranged from 25 to 30°C with the annual mean rainfall, which is between 900 and 1500 mm. Atacora and Alibori have a climate with two seasons, one rainy season (May-October) and one dry season (November- April).The temperature ranged from 23 to 33°C with the annual mean rainfall, which is 1300 mm.


Mixed-function oxidases and esterases associated with permethrin, deltamethrin and bendiocarb resistance in Anopheles gambiae s.l. in the south-north transect Benin, West Africa.

Aïzoun N, Aïkpon R, Padonou GG, Oussou O, Oké-Agbo F, Gnanguenon V, Ossè R, Akogbéto M - Parasit Vectors (2013)

Map of the study area.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750545&req=5

Figure 1: Map of the study area.
Mentions: The study was carried out in the South of Benin, more precisely in Agbalilame, in the Seme district of Oueme province and in the North of Benin, in the Tanguieta district of Atacora province and in the Kandi district of Alibori province (Figure 1). The choice of the study sites took into account the economic activities of populations, their usual protection practices against mosquito bites, the indoor residual spraying (IRS) in progress in certain of these localities and peasant practices to control farming pests. These factors have an impact on the resistance development in the local vector mosquitoes. Oueme has a climate with four seasons, two rainy seasons (March-July and September-November) and two dry seasons (December-March and August-September).The temperature ranged from 25 to 30°C with the annual mean rainfall, which is between 900 and 1500 mm. Atacora and Alibori have a climate with two seasons, one rainy season (May-October) and one dry season (November- April).The temperature ranged from 23 to 33°C with the annual mean rainfall, which is 1300 mm.

Bottom Line: The susceptibility of Anopheles gambiae Agbalilame and Kandi populations to permethrin and deltamethrin respectively, increased significantly when synergized by PBO, suggesting an implication of mono-oxygenases in resistance of Anopheles gambiae s.l. to pyrethroid.Esterases may play a role in bendiocarb resistance in Anopheles gambiae Tanguieta.Synergists partially restored susceptibility to pyrethroid and carbamate insecticides and might help mitigate the impact of vector resistance in Anopheles gambiae Agbalilame, Kandi and Tanguieta populations.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Insecticide resistance monitoring is essential to help national programmers to implement more effective and sustainable malaria control strategies in endemic countries. The current study aimed at an exploring the involvement of detoxifying enzymes in the insecticide phenotype resistance in Anopheles gambiae s.l.from Benin, in order to guide future malaria vector control interventions.

Methods: Larvae and pupae of Anopheles gambiae s.l. mosquitoes were collected from the breeding sites in Oueme, Atacora and Alibori provinces. CDC susceptibility tests were conducted on unfed female mosquitoes aged 2-5 days old. CDC bioassays were performed with stock solutions of permethrin (21.5 μg per bottle), deltamethrin (12.5 μg per bottle) and bendiocarb (12.5 μg per bottle). CDC biochemical assays using synergists were also conducted to assess the metabolic resistance.

Results: The susceptibility of Anopheles gambiae Agbalilame and Kandi populations to permethrin and deltamethrin respectively, increased significantly when synergized by PBO, suggesting an implication of mono-oxygenases in resistance of Anopheles gambiae s.l. to pyrethroid. Esterases may play a role in bendiocarb resistance in Anopheles gambiae Tanguieta.

Conclusion: Synergists partially restored susceptibility to pyrethroid and carbamate insecticides and might help mitigate the impact of vector resistance in Anopheles gambiae Agbalilame, Kandi and Tanguieta populations. However, additional vector control tools are needed to further impact on malaria transmission in such settings.This will improve the implementation and management of future control programs against this important malaria vector in Benin and in Africa in general.

Show MeSH
Related in: MedlinePlus