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Direct characterization of bulk samples by internal extractive electrospray ionization mass spectrometry.

Zhang H, Gu H, Yan F, Wang N, Wei Y, Xu J, Chen H - Sci Rep (2013)

Bottom Line: The method allows both qualitative and quantitative analysis of analytes distributed in a 3-dimensional volume (e.g., 1 ~ 100 mm(3)) of various synthetic and biological matrices (e.g., chewing gum, leaves, fruits, roots, pork, lung tissues) without either mashing the sample or matrix separation.Using different extraction solvents, online chromatographic separation of chemicals inside the sample volume was observed during iEESI-MS analysis.The presented method is featured by the high speed of analysis, high sensitivity, low sample consumption and minimal sample preparation and/or degradation, offering unique possibilities for advanced applications in plant science, clinical diagnosis, catalyst studies, and materials science.

View Article: PubMed Central - PubMed

Affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.

ABSTRACT
A straight-forward analytical strategy called internal extractive electrospray ionization mass spectrometry (iEESI-MS), which combines solvent extraction of chemicals inside a bulk sample with in situ electrospray ionization mass spectrometry, has been established to directly characterize the interior of a bulk sample with molecular specificity. The method allows both qualitative and quantitative analysis of analytes distributed in a 3-dimensional volume (e.g., 1 ~ 100 mm(3)) of various synthetic and biological matrices (e.g., chewing gum, leaves, fruits, roots, pork, lung tissues) without either mashing the sample or matrix separation. Using different extraction solvents, online chromatographic separation of chemicals inside the sample volume was observed during iEESI-MS analysis. The presented method is featured by the high speed of analysis, high sensitivity, low sample consumption and minimal sample preparation and/or degradation, offering unique possibilities for advanced applications in plant science, clinical diagnosis, catalyst studies, and materials science.

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Conceptual illustration of internal extractive electrospray ionization (iEESI).
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f1: Conceptual illustration of internal extractive electrospray ionization (iEESI).

Mentions: Herein we report a novel strategy termed as internal extractive electrospray ionization (iEESI) for the straight-forward MS analysis of whole-volume (typically ≥ 20 mm3) samples (e.g., food, biological tissues) without either mashing/grinding the sample or matrix clean-up. In iEESI, extraction solution (e.g., methanol, water, etc.) charged at a high voltage (±4.5 kV) is directly infused into the 3-dimensional volume of the analyzed sample (e.g., a tissue, leaf, fruit, etc.) through the inserted capillary. The analytes are extracted by the infused solvent and carried along the electric field gradient inside the bulk volume of the sample. Stable electrospray plume is generated from the opposite sample edge, in front of MS inlet (Fig. 1). Following the iEESI strategy, molecular information of analytes distributed in a bulk volume (1 ~ 100 mm3) of a sample, rather than merely from its surface, can be revealed. The unique mechanism and performance characteristics can render iEESI-MS useful for the direct characterization of bulk samples without affecting their integrity.


Direct characterization of bulk samples by internal extractive electrospray ionization mass spectrometry.

Zhang H, Gu H, Yan F, Wang N, Wei Y, Xu J, Chen H - Sci Rep (2013)

Conceptual illustration of internal extractive electrospray ionization (iEESI).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750536&req=5

f1: Conceptual illustration of internal extractive electrospray ionization (iEESI).
Mentions: Herein we report a novel strategy termed as internal extractive electrospray ionization (iEESI) for the straight-forward MS analysis of whole-volume (typically ≥ 20 mm3) samples (e.g., food, biological tissues) without either mashing/grinding the sample or matrix clean-up. In iEESI, extraction solution (e.g., methanol, water, etc.) charged at a high voltage (±4.5 kV) is directly infused into the 3-dimensional volume of the analyzed sample (e.g., a tissue, leaf, fruit, etc.) through the inserted capillary. The analytes are extracted by the infused solvent and carried along the electric field gradient inside the bulk volume of the sample. Stable electrospray plume is generated from the opposite sample edge, in front of MS inlet (Fig. 1). Following the iEESI strategy, molecular information of analytes distributed in a bulk volume (1 ~ 100 mm3) of a sample, rather than merely from its surface, can be revealed. The unique mechanism and performance characteristics can render iEESI-MS useful for the direct characterization of bulk samples without affecting their integrity.

Bottom Line: The method allows both qualitative and quantitative analysis of analytes distributed in a 3-dimensional volume (e.g., 1 ~ 100 mm(3)) of various synthetic and biological matrices (e.g., chewing gum, leaves, fruits, roots, pork, lung tissues) without either mashing the sample or matrix separation.Using different extraction solvents, online chromatographic separation of chemicals inside the sample volume was observed during iEESI-MS analysis.The presented method is featured by the high speed of analysis, high sensitivity, low sample consumption and minimal sample preparation and/or degradation, offering unique possibilities for advanced applications in plant science, clinical diagnosis, catalyst studies, and materials science.

View Article: PubMed Central - PubMed

Affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.

ABSTRACT
A straight-forward analytical strategy called internal extractive electrospray ionization mass spectrometry (iEESI-MS), which combines solvent extraction of chemicals inside a bulk sample with in situ electrospray ionization mass spectrometry, has been established to directly characterize the interior of a bulk sample with molecular specificity. The method allows both qualitative and quantitative analysis of analytes distributed in a 3-dimensional volume (e.g., 1 ~ 100 mm(3)) of various synthetic and biological matrices (e.g., chewing gum, leaves, fruits, roots, pork, lung tissues) without either mashing the sample or matrix separation. Using different extraction solvents, online chromatographic separation of chemicals inside the sample volume was observed during iEESI-MS analysis. The presented method is featured by the high speed of analysis, high sensitivity, low sample consumption and minimal sample preparation and/or degradation, offering unique possibilities for advanced applications in plant science, clinical diagnosis, catalyst studies, and materials science.

Show MeSH
Related in: MedlinePlus