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The mouse and ferret models for studying the novel avian-origin human influenza A (H7N9) virus.

Xu L, Bao L, Deng W, Zhu H, Chen T, Lv Q, Li F, Yuan J, Xiang Z, Gao K, Xu Y, Huang L, Li Y, Liu J, Yao Y, Yu P, Yong W, Wei Q, Zhang L, Qin C - Virol. J. (2013)

Bottom Line: Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets.Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comptive Medicine Center, Peking Union Medical Collage (PUMC), Key Laboratory of Human Disease Comptive Medicine, Ministry of Health, Beijing, China.

ABSTRACT

Background: The current study was conducted to establish animal models (including mouse and ferret) for the novel avian-origin H7N9 influenza virus.

Findings: A/Anhui/1/2013 (H7N9) virus was administered by intranasal instillation to groups of mice and ferrets, and animals developed typical clinical signs including body weight loss (mice and ferrets), ruffled fur (mice), sneezing (ferrets), and death (mice). Peak virus shedding from respiratory tract was observed on 2 days post inoculation (d.p.i.) for mice and 3-5 d.p.i. for ferrets. Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets. The inoculation of H7N9 could elicit seroconversion titers up to 1280 in ferrets and 160 in mice. Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.

Conclusions: The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

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Histopathological analyses of tissues of inoculated mice and ferrets. (A) Hematoxylin and Eosin (H-E) stain and immunohistochemical (IHC) analyses of tissues of inoculated mice. (B) Hematoxylin and Eosin (H-E) stain and IHC analyses of lungs of inoculated ferrets.
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Figure 4: Histopathological analyses of tissues of inoculated mice and ferrets. (A) Hematoxylin and Eosin (H-E) stain and immunohistochemical (IHC) analyses of tissues of inoculated mice. (B) Hematoxylin and Eosin (H-E) stain and IHC analyses of lungs of inoculated ferrets.

Mentions: The dissemination of virus in inoculated mice were also assessed by immunohistochemical analysis (IHC), the viral antigens were mainly located within the epithelial cells of the bronchial in the lung, the choroid plexus in the brain, the villous column in the small intestine, and the renal tubules in the kidney (Figure 4A (i-iv)). Meanwhile, all tissues of inoculated mice were subjected to pathological analysis. Characterization of inflammation in the lungs revealed that from 1 d.p.i., lung tissues exhibited characteristic pathology of influenza infection, including inflammatory hyperaemia and exudative pathological changes. From 3 to 7 d.p.i., the lesions of lung tissue became larger, and more severe interstitial pneumonia were observed (Figure 4A (v-viii)).


The mouse and ferret models for studying the novel avian-origin human influenza A (H7N9) virus.

Xu L, Bao L, Deng W, Zhu H, Chen T, Lv Q, Li F, Yuan J, Xiang Z, Gao K, Xu Y, Huang L, Li Y, Liu J, Yao Y, Yu P, Yong W, Wei Q, Zhang L, Qin C - Virol. J. (2013)

Histopathological analyses of tissues of inoculated mice and ferrets. (A) Hematoxylin and Eosin (H-E) stain and immunohistochemical (IHC) analyses of tissues of inoculated mice. (B) Hematoxylin and Eosin (H-E) stain and IHC analyses of lungs of inoculated ferrets.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750532&req=5

Figure 4: Histopathological analyses of tissues of inoculated mice and ferrets. (A) Hematoxylin and Eosin (H-E) stain and immunohistochemical (IHC) analyses of tissues of inoculated mice. (B) Hematoxylin and Eosin (H-E) stain and IHC analyses of lungs of inoculated ferrets.
Mentions: The dissemination of virus in inoculated mice were also assessed by immunohistochemical analysis (IHC), the viral antigens were mainly located within the epithelial cells of the bronchial in the lung, the choroid plexus in the brain, the villous column in the small intestine, and the renal tubules in the kidney (Figure 4A (i-iv)). Meanwhile, all tissues of inoculated mice were subjected to pathological analysis. Characterization of inflammation in the lungs revealed that from 1 d.p.i., lung tissues exhibited characteristic pathology of influenza infection, including inflammatory hyperaemia and exudative pathological changes. From 3 to 7 d.p.i., the lesions of lung tissue became larger, and more severe interstitial pneumonia were observed (Figure 4A (v-viii)).

Bottom Line: Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets.Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comptive Medicine Center, Peking Union Medical Collage (PUMC), Key Laboratory of Human Disease Comptive Medicine, Ministry of Health, Beijing, China.

ABSTRACT

Background: The current study was conducted to establish animal models (including mouse and ferret) for the novel avian-origin H7N9 influenza virus.

Findings: A/Anhui/1/2013 (H7N9) virus was administered by intranasal instillation to groups of mice and ferrets, and animals developed typical clinical signs including body weight loss (mice and ferrets), ruffled fur (mice), sneezing (ferrets), and death (mice). Peak virus shedding from respiratory tract was observed on 2 days post inoculation (d.p.i.) for mice and 3-5 d.p.i. for ferrets. Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets. The inoculation of H7N9 could elicit seroconversion titers up to 1280 in ferrets and 160 in mice. Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.

Conclusions: The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

Show MeSH
Related in: MedlinePlus