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The mouse and ferret models for studying the novel avian-origin human influenza A (H7N9) virus.

Xu L, Bao L, Deng W, Zhu H, Chen T, Lv Q, Li F, Yuan J, Xiang Z, Gao K, Xu Y, Huang L, Li Y, Liu J, Yao Y, Yu P, Yong W, Wei Q, Zhang L, Qin C - Virol. J. (2013)

Bottom Line: Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets.Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comptive Medicine Center, Peking Union Medical Collage (PUMC), Key Laboratory of Human Disease Comptive Medicine, Ministry of Health, Beijing, China.

ABSTRACT

Background: The current study was conducted to establish animal models (including mouse and ferret) for the novel avian-origin H7N9 influenza virus.

Findings: A/Anhui/1/2013 (H7N9) virus was administered by intranasal instillation to groups of mice and ferrets, and animals developed typical clinical signs including body weight loss (mice and ferrets), ruffled fur (mice), sneezing (ferrets), and death (mice). Peak virus shedding from respiratory tract was observed on 2 days post inoculation (d.p.i.) for mice and 3-5 d.p.i. for ferrets. Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets. The inoculation of H7N9 could elicit seroconversion titers up to 1280 in ferrets and 160 in mice. Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.

Conclusions: The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

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Virus shedding of inoculated BALB/c mice or ferrets with H7N9 virus. (A) Virus shedding in the BALF and lung tissues of inoculated mice. (B) Virus shedding in the nasal and throat swabs of inoculated ferrets. Data are presented as mean value ± SD. * P<0.05.
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Figure 3: Virus shedding of inoculated BALB/c mice or ferrets with H7N9 virus. (A) Virus shedding in the BALF and lung tissues of inoculated mice. (B) Virus shedding in the nasal and throat swabs of inoculated ferrets. Data are presented as mean value ± SD. * P<0.05.

Mentions: The virus dissemination in the bronchoalveolar lavage fluid (BALF), lung, and other main tissues (heart, liver, spleen, kidney, intestine, and brain) of inoculated mice were titrated on MDCK cell. Virus shedding was observed to start on 1 d.p.i. and continued until 7 d.p.i. in both BALF and lungs, with lung tissues containing higher virus titers than BALF from 2 d.p.i. (P<0.05). The peak virus shedding reached 103.75 TCID50 for BALF on 1 d.p.i. and 105.69 TCID50 for lung on 2 d.p.i. (Figure 3A and Table 1). Meanwhile, virus could also be isolated from the brain, liver, spleen, kidney and intestine (Table 1). The brain tropism of H7N9 virus was coincidence with other highly pathogenic avian viruses such as H5N1and H7N7 [2-4].


The mouse and ferret models for studying the novel avian-origin human influenza A (H7N9) virus.

Xu L, Bao L, Deng W, Zhu H, Chen T, Lv Q, Li F, Yuan J, Xiang Z, Gao K, Xu Y, Huang L, Li Y, Liu J, Yao Y, Yu P, Yong W, Wei Q, Zhang L, Qin C - Virol. J. (2013)

Virus shedding of inoculated BALB/c mice or ferrets with H7N9 virus. (A) Virus shedding in the BALF and lung tissues of inoculated mice. (B) Virus shedding in the nasal and throat swabs of inoculated ferrets. Data are presented as mean value ± SD. * P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750532&req=5

Figure 3: Virus shedding of inoculated BALB/c mice or ferrets with H7N9 virus. (A) Virus shedding in the BALF and lung tissues of inoculated mice. (B) Virus shedding in the nasal and throat swabs of inoculated ferrets. Data are presented as mean value ± SD. * P<0.05.
Mentions: The virus dissemination in the bronchoalveolar lavage fluid (BALF), lung, and other main tissues (heart, liver, spleen, kidney, intestine, and brain) of inoculated mice were titrated on MDCK cell. Virus shedding was observed to start on 1 d.p.i. and continued until 7 d.p.i. in both BALF and lungs, with lung tissues containing higher virus titers than BALF from 2 d.p.i. (P<0.05). The peak virus shedding reached 103.75 TCID50 for BALF on 1 d.p.i. and 105.69 TCID50 for lung on 2 d.p.i. (Figure 3A and Table 1). Meanwhile, virus could also be isolated from the brain, liver, spleen, kidney and intestine (Table 1). The brain tropism of H7N9 virus was coincidence with other highly pathogenic avian viruses such as H5N1and H7N7 [2-4].

Bottom Line: Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets.Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comptive Medicine Center, Peking Union Medical Collage (PUMC), Key Laboratory of Human Disease Comptive Medicine, Ministry of Health, Beijing, China.

ABSTRACT

Background: The current study was conducted to establish animal models (including mouse and ferret) for the novel avian-origin H7N9 influenza virus.

Findings: A/Anhui/1/2013 (H7N9) virus was administered by intranasal instillation to groups of mice and ferrets, and animals developed typical clinical signs including body weight loss (mice and ferrets), ruffled fur (mice), sneezing (ferrets), and death (mice). Peak virus shedding from respiratory tract was observed on 2 days post inoculation (d.p.i.) for mice and 3-5 d.p.i. for ferrets. Virus could also be detected in brain, liver, spleen, kidney, and intestine from inoculated mice, and in heart, liver, and olfactory bulb from inoculated ferrets. The inoculation of H7N9 could elicit seroconversion titers up to 1280 in ferrets and 160 in mice. Leukopenia, significantly reduced lymphocytes but increased neutrophils were also observed in mouse and ferret models.

Conclusions: The mouse and ferret model enables detailed studies of the pathogenesis of this illness and lay the foundation for drug or vaccine evaluation.

Show MeSH
Related in: MedlinePlus