Limits...
Enhancement of mitochondrial biogenesis with polyphenols: combined effects of resveratrol and equol in human endothelial cells.

Davinelli S, Sapere N, Visentin M, Zella D, Scapagnini G - Immun Ageing (2013)

Bottom Line: Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline.Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function.In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine and Health Sciences, University of Molise, Campobasso 86100, Italy. g.scapagnini@gmail.com.

ABSTRACT
Emerging evidence suggests that combinatorial action of numerous biologically active compounds may be a valuable source in a variety of therapeutic applications. Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline. Recently, the possibilities of anti-ageing interventions have multiplied also to ameliorate the mitochondrial alterations in ageing-associated diseases. In this report, we approached a novel treatment strategy by combining two bioactive dietary constituents (resveratrol and equol) to determine their effect on mitochondrial function. Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function. In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content. Additionally, combined use of both compounds increased SIRT1 enzymatic activity and induced mitochondrial biogenesis factors such as PGC1-α, TFAM and NRF-1. Therefore, identification of this novel synergism may provide a new perspective for future treatments aiming to modulate the mitochondrial activity with implications in maintaining endothelial function which is crucial in the regulation of immune response. Further studies to discover the molecular details of this crosstalk and to identify new combinations of active compounds affecting the mitochondrial function will be extremely beneficial to prevent mitochondrial decline.

No MeSH data available.


Related in: MedlinePlus

Effect of resveratrol and equol on mRNA expression of PGC1-α (A), NRF-1 (B), TFAM (C) in HUVEC. qRT-PCR measurement to assess the mRNA expression of the mitochondrial biogenesis factors. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750512&req=5

Figure 3: Effect of resveratrol and equol on mRNA expression of PGC1-α (A), NRF-1 (B), TFAM (C) in HUVEC. qRT-PCR measurement to assess the mRNA expression of the mitochondrial biogenesis factors. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.

Mentions: PGC1-α is a key component in modulating mitochondrial function and interacts with transcription factors such as NRF-1and TFAM. Specifically, PGC1-α is involved in regulating the expression of mtDNA via increased expression of TFAM which is co-activated by NRF-1 [27]. Moreover, NRF-1 in addition to regulating the activation of TFAM is also a downstream effector of SIRT1/PGC1-α [28]. Accordingly we used qRT-PCR measurements to analyse the expression of PGC1-α, NRF-1and TFAM. The results indicate that these mitochondrial biogenesis factors were strongly increased by combined treatment of resveratrol and equol (Figure 3).


Enhancement of mitochondrial biogenesis with polyphenols: combined effects of resveratrol and equol in human endothelial cells.

Davinelli S, Sapere N, Visentin M, Zella D, Scapagnini G - Immun Ageing (2013)

Effect of resveratrol and equol on mRNA expression of PGC1-α (A), NRF-1 (B), TFAM (C) in HUVEC. qRT-PCR measurement to assess the mRNA expression of the mitochondrial biogenesis factors. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750512&req=5

Figure 3: Effect of resveratrol and equol on mRNA expression of PGC1-α (A), NRF-1 (B), TFAM (C) in HUVEC. qRT-PCR measurement to assess the mRNA expression of the mitochondrial biogenesis factors. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
Mentions: PGC1-α is a key component in modulating mitochondrial function and interacts with transcription factors such as NRF-1and TFAM. Specifically, PGC1-α is involved in regulating the expression of mtDNA via increased expression of TFAM which is co-activated by NRF-1 [27]. Moreover, NRF-1 in addition to regulating the activation of TFAM is also a downstream effector of SIRT1/PGC1-α [28]. Accordingly we used qRT-PCR measurements to analyse the expression of PGC1-α, NRF-1and TFAM. The results indicate that these mitochondrial biogenesis factors were strongly increased by combined treatment of resveratrol and equol (Figure 3).

Bottom Line: Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline.Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function.In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine and Health Sciences, University of Molise, Campobasso 86100, Italy. g.scapagnini@gmail.com.

ABSTRACT
Emerging evidence suggests that combinatorial action of numerous biologically active compounds may be a valuable source in a variety of therapeutic applications. Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline. Recently, the possibilities of anti-ageing interventions have multiplied also to ameliorate the mitochondrial alterations in ageing-associated diseases. In this report, we approached a novel treatment strategy by combining two bioactive dietary constituents (resveratrol and equol) to determine their effect on mitochondrial function. Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function. In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content. Additionally, combined use of both compounds increased SIRT1 enzymatic activity and induced mitochondrial biogenesis factors such as PGC1-α, TFAM and NRF-1. Therefore, identification of this novel synergism may provide a new perspective for future treatments aiming to modulate the mitochondrial activity with implications in maintaining endothelial function which is crucial in the regulation of immune response. Further studies to discover the molecular details of this crosstalk and to identify new combinations of active compounds affecting the mitochondrial function will be extremely beneficial to prevent mitochondrial decline.

No MeSH data available.


Related in: MedlinePlus