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Enhancement of mitochondrial biogenesis with polyphenols: combined effects of resveratrol and equol in human endothelial cells.

Davinelli S, Sapere N, Visentin M, Zella D, Scapagnini G - Immun Ageing (2013)

Bottom Line: Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline.Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function.In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine and Health Sciences, University of Molise, Campobasso 86100, Italy. g.scapagnini@gmail.com.

ABSTRACT
Emerging evidence suggests that combinatorial action of numerous biologically active compounds may be a valuable source in a variety of therapeutic applications. Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline. Recently, the possibilities of anti-ageing interventions have multiplied also to ameliorate the mitochondrial alterations in ageing-associated diseases. In this report, we approached a novel treatment strategy by combining two bioactive dietary constituents (resveratrol and equol) to determine their effect on mitochondrial function. Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function. In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content. Additionally, combined use of both compounds increased SIRT1 enzymatic activity and induced mitochondrial biogenesis factors such as PGC1-α, TFAM and NRF-1. Therefore, identification of this novel synergism may provide a new perspective for future treatments aiming to modulate the mitochondrial activity with implications in maintaining endothelial function which is crucial in the regulation of immune response. Further studies to discover the molecular details of this crosstalk and to identify new combinations of active compounds affecting the mitochondrial function will be extremely beneficial to prevent mitochondrial decline.

No MeSH data available.


Related in: MedlinePlus

Increase of SIRT1 enzymatic activity by combined administration of resveratrol and equol. Fluorimetric SIRT1 activity assay to determine the effect achieved in HUVEC by the combined exposure to equol and resveratrol. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). AFU: arbitrary fluorescence units; C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
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Figure 2: Increase of SIRT1 enzymatic activity by combined administration of resveratrol and equol. Fluorimetric SIRT1 activity assay to determine the effect achieved in HUVEC by the combined exposure to equol and resveratrol. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). AFU: arbitrary fluorescence units; C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.

Mentions: It has been consistently demonstrated that activation of SIRT1 stimulates mitochondrial biogenesis and resveratrol has been utilized as a SIRT1 activator to regulate mitochondrial function [26]. Since equol exhibits a wide range of biological properties, it may be a sirtuin-targeting nutraceutical to prevent mitochondrial decline. Analysis of the effect of a combined administration of resveratrol and equol showed an increase in SIRT1 enzymatic activity in HUVEC endothelial cells (Figure 2). Although equol was much less effective to induce SIRT1, the effect of a combination of resveratrol and equol was greater than the response achieved by the single compounds (Figure 2). Therefore, the combination of resveratrol with equol is also associated with the activation of SIRT1.


Enhancement of mitochondrial biogenesis with polyphenols: combined effects of resveratrol and equol in human endothelial cells.

Davinelli S, Sapere N, Visentin M, Zella D, Scapagnini G - Immun Ageing (2013)

Increase of SIRT1 enzymatic activity by combined administration of resveratrol and equol. Fluorimetric SIRT1 activity assay to determine the effect achieved in HUVEC by the combined exposure to equol and resveratrol. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). AFU: arbitrary fluorescence units; C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750512&req=5

Figure 2: Increase of SIRT1 enzymatic activity by combined administration of resveratrol and equol. Fluorimetric SIRT1 activity assay to determine the effect achieved in HUVEC by the combined exposure to equol and resveratrol. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). AFU: arbitrary fluorescence units; C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
Mentions: It has been consistently demonstrated that activation of SIRT1 stimulates mitochondrial biogenesis and resveratrol has been utilized as a SIRT1 activator to regulate mitochondrial function [26]. Since equol exhibits a wide range of biological properties, it may be a sirtuin-targeting nutraceutical to prevent mitochondrial decline. Analysis of the effect of a combined administration of resveratrol and equol showed an increase in SIRT1 enzymatic activity in HUVEC endothelial cells (Figure 2). Although equol was much less effective to induce SIRT1, the effect of a combination of resveratrol and equol was greater than the response achieved by the single compounds (Figure 2). Therefore, the combination of resveratrol with equol is also associated with the activation of SIRT1.

Bottom Line: Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline.Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function.In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine and Health Sciences, University of Molise, Campobasso 86100, Italy. g.scapagnini@gmail.com.

ABSTRACT
Emerging evidence suggests that combinatorial action of numerous biologically active compounds may be a valuable source in a variety of therapeutic applications. Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline. Recently, the possibilities of anti-ageing interventions have multiplied also to ameliorate the mitochondrial alterations in ageing-associated diseases. In this report, we approached a novel treatment strategy by combining two bioactive dietary constituents (resveratrol and equol) to determine their effect on mitochondrial function. Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function. In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content. Additionally, combined use of both compounds increased SIRT1 enzymatic activity and induced mitochondrial biogenesis factors such as PGC1-α, TFAM and NRF-1. Therefore, identification of this novel synergism may provide a new perspective for future treatments aiming to modulate the mitochondrial activity with implications in maintaining endothelial function which is crucial in the regulation of immune response. Further studies to discover the molecular details of this crosstalk and to identify new combinations of active compounds affecting the mitochondrial function will be extremely beneficial to prevent mitochondrial decline.

No MeSH data available.


Related in: MedlinePlus