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Enhancement of mitochondrial biogenesis with polyphenols: combined effects of resveratrol and equol in human endothelial cells.

Davinelli S, Sapere N, Visentin M, Zella D, Scapagnini G - Immun Ageing (2013)

Bottom Line: Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline.Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function.In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine and Health Sciences, University of Molise, Campobasso 86100, Italy. g.scapagnini@gmail.com.

ABSTRACT
Emerging evidence suggests that combinatorial action of numerous biologically active compounds may be a valuable source in a variety of therapeutic applications. Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline. Recently, the possibilities of anti-ageing interventions have multiplied also to ameliorate the mitochondrial alterations in ageing-associated diseases. In this report, we approached a novel treatment strategy by combining two bioactive dietary constituents (resveratrol and equol) to determine their effect on mitochondrial function. Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function. In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content. Additionally, combined use of both compounds increased SIRT1 enzymatic activity and induced mitochondrial biogenesis factors such as PGC1-α, TFAM and NRF-1. Therefore, identification of this novel synergism may provide a new perspective for future treatments aiming to modulate the mitochondrial activity with implications in maintaining endothelial function which is crucial in the regulation of immune response. Further studies to discover the molecular details of this crosstalk and to identify new combinations of active compounds affecting the mitochondrial function will be extremely beneficial to prevent mitochondrial decline.

No MeSH data available.


Related in: MedlinePlus

The combined treatment of resveratrol and equol strongly increased the number of mitochondria in HUVEC cells. (A) Mitotracker fluorescent intensities were analysed to assess the mitochondrial biogenesis. (B) Relative mitochondrial DNA (mtDNA) content was estimated by qRT-PCR. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
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Figure 1: The combined treatment of resveratrol and equol strongly increased the number of mitochondria in HUVEC cells. (A) Mitotracker fluorescent intensities were analysed to assess the mitochondrial biogenesis. (B) Relative mitochondrial DNA (mtDNA) content was estimated by qRT-PCR. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.

Mentions: In the current study, we examined the combined treatment of resveratrol and equol on HUVEC cells to identify their effects on the mitochondrial biogenesis. In particular, the primary goal of this investigation was to determine whether combining resveratrol and equol would increase the expression of key factors involved in mitochondrial biogenesis. Several studies were conducted showing the synergistic effect of resveratrol with different compounds [20] but the combination with equol has never been tested on the mitochondrial function. The Mitotracker staining showed that mitochondria were located in the perinuclear region in HUVEC (data not shown). Treatment with resveratrol increased significantly the density-area ratio in Mitotracker-labeled endothelial cells as compared to the cytoplasmatic volume (Figure 1A).


Enhancement of mitochondrial biogenesis with polyphenols: combined effects of resveratrol and equol in human endothelial cells.

Davinelli S, Sapere N, Visentin M, Zella D, Scapagnini G - Immun Ageing (2013)

The combined treatment of resveratrol and equol strongly increased the number of mitochondria in HUVEC cells. (A) Mitotracker fluorescent intensities were analysed to assess the mitochondrial biogenesis. (B) Relative mitochondrial DNA (mtDNA) content was estimated by qRT-PCR. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750512&req=5

Figure 1: The combined treatment of resveratrol and equol strongly increased the number of mitochondria in HUVEC cells. (A) Mitotracker fluorescent intensities were analysed to assess the mitochondrial biogenesis. (B) Relative mitochondrial DNA (mtDNA) content was estimated by qRT-PCR. Representative data of at least 3 experiments each performed in triplicate. (*= P < 0.05, **= P < 0.01). C: control; E: equol; R: resveratrol; E+R: equol + resveratrol.
Mentions: In the current study, we examined the combined treatment of resveratrol and equol on HUVEC cells to identify their effects on the mitochondrial biogenesis. In particular, the primary goal of this investigation was to determine whether combining resveratrol and equol would increase the expression of key factors involved in mitochondrial biogenesis. Several studies were conducted showing the synergistic effect of resveratrol with different compounds [20] but the combination with equol has never been tested on the mitochondrial function. The Mitotracker staining showed that mitochondria were located in the perinuclear region in HUVEC (data not shown). Treatment with resveratrol increased significantly the density-area ratio in Mitotracker-labeled endothelial cells as compared to the cytoplasmatic volume (Figure 1A).

Bottom Line: Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline.Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function.In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine and Health Sciences, University of Molise, Campobasso 86100, Italy. g.scapagnini@gmail.com.

ABSTRACT
Emerging evidence suggests that combinatorial action of numerous biologically active compounds may be a valuable source in a variety of therapeutic applications. Several nutraceuticals have demonstrated to augment the efficacy of pharmacological approaches or provide physiological benefit to improve age-related decline. Recently, the possibilities of anti-ageing interventions have multiplied also to ameliorate the mitochondrial alterations in ageing-associated diseases. In this report, we approached a novel treatment strategy by combining two bioactive dietary constituents (resveratrol and equol) to determine their effect on mitochondrial function. Taking into account that the biological activities of resveratrol and equol has been observed in a wide range of biological processes, they were selected to examine whether combining them would be more effective to modulate mitochondrial function. In HUVEC cells our results demonstrate that the co-administration of these natural products increased mitochondrial mass and mitochondrial DNA content. Additionally, combined use of both compounds increased SIRT1 enzymatic activity and induced mitochondrial biogenesis factors such as PGC1-α, TFAM and NRF-1. Therefore, identification of this novel synergism may provide a new perspective for future treatments aiming to modulate the mitochondrial activity with implications in maintaining endothelial function which is crucial in the regulation of immune response. Further studies to discover the molecular details of this crosstalk and to identify new combinations of active compounds affecting the mitochondrial function will be extremely beneficial to prevent mitochondrial decline.

No MeSH data available.


Related in: MedlinePlus