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Analysis of nine compounds from Alpinia oxyphylla fruit at different harvest time using UFLC-MS/MS and an extraction method optimized by orthogonal design.

Li YH, Chen F, Wang JF, Wang Y, Zhang JQ, Guo T - Chem Cent J (2013)

Bottom Line: The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min.The contents of the six bioactive compounds were reported in A. oxyphylla for the first time.The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Hainan Provincial Key Laboratory of R&D on Tropical Herbs, Hainan Medical University, Haikou 571199, China ; School of Pharmacy, Hainan Medical University, Haikou, Hainan 571199, China.

ABSTRACT

Background: The dried fruits of Alpinia oxyphylla Miq have been widely used as an herbal medicine for the treatment of diarrhea and enuresis in China. Medicinal chemistry studies revealed that the tepenes, diphenylheptanes and flavones were the main components. Therefore, these three kinds of components should be chosen as the bioactive marker compounds for the quality control of A. oxyphylla fruits. Moreover, multiple active components has been widely recognized to be a more feasible method for the quality control of herbal medicines. This study firstly provided a better method for comprehensive component analysis of A. oxyphylla fruits. Meanwhile, the different harvest time was also evaluated.

Results: The solvent-to-sample ratio was the most important factor comparing with solvent, extraction time and temperature. The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min. The optimized analytical method for ultra fast high performance liquid chromatography (UFLC) was a gradient elution using water containing 0.04‰ formic acid (A) and methanol containing 0.04‰ formic acid (B), at a flow rate of 0.3 mL/min. Using this optimized method, nine compounds were simultaneously separated and quantified by UFLC coupled with tandem electro-spray ionized mass spectrometry (MS/MS).

Conclusions: The contents of the six bioactive compounds were reported in A. oxyphylla for the first time. The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

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Related in: MedlinePlus

The content levels of nine compounds of different harvest time fruits of Alipina oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest levels.
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Figure 3: The content levels of nine compounds of different harvest time fruits of Alipina oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest levels.

Mentions: The proposed UFLC-MS/MS method was subsequently applied to determine nine chemical markers including nootkatone (1), yakuchinone A (2), yakuchinone B (3), oxyphyllacinol (4), tectochrysin (5), izalpinin (6), chrysin (7), kaempferide (8) and apigenin-7,4′-dimethylether (9) from the 10-day to 65-day samples of fruits. The results (Table 5 and Figure 3) showed that three kinds of components accumulated with the growth time. But there were remarkable differences in their contents at different harvest time. For example, there was few nootkatone before 27 days in fruits. With the growth of fruit, the content of nootkatone entered a rapid growth phase and reached the maximum (mean value as 1.557 mg/g) at 45-day. From 45-day to 55-day, the content of nootkatone in fruits showed a little downward trend from 1.557 mg/g to 1.160 mg/g. For the three diphenylheptanes compounds, the content of every compound was very low before 40-day but at 45-day all their contents increased sharply to the maximum (mean value as 2.401 mg/g for yakuchinone A, 0.0795 mg/g for yakuchinone B and 2.465 mg/g for oxyphyllacinol). After 45-day, the contents of three diphenylheptanes compounds declined to 503 μg/g for yakuchinone A, 40 μg/g for yakuchinone B and 353 μg/g for oxyphyllacinol at 65-day. The flavones compounds exhibited another characteristics. Most of flavones achieved the maximum content before 30 harvest time. The tectochrysin had the maximum content of 128 μg/g at 20-day, the izalpinin of 33.2 μg/g at 20-day, the kaempferide of 264 μg/g at 15-day and apigenin-7,4′-dimethylether of 86.7 μg/g at 15-day. Only the content of chrysin achieved the maximum of 62.9 μg/g at 45-day. The differences of content variation between flavones with other two kinds of compounds might indicate the biosynthetic pathway of flavones was differentiated with the other two types of phytochemicals.


Analysis of nine compounds from Alpinia oxyphylla fruit at different harvest time using UFLC-MS/MS and an extraction method optimized by orthogonal design.

Li YH, Chen F, Wang JF, Wang Y, Zhang JQ, Guo T - Chem Cent J (2013)

The content levels of nine compounds of different harvest time fruits of Alipina oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest levels.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750461&req=5

Figure 3: The content levels of nine compounds of different harvest time fruits of Alipina oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest levels.
Mentions: The proposed UFLC-MS/MS method was subsequently applied to determine nine chemical markers including nootkatone (1), yakuchinone A (2), yakuchinone B (3), oxyphyllacinol (4), tectochrysin (5), izalpinin (6), chrysin (7), kaempferide (8) and apigenin-7,4′-dimethylether (9) from the 10-day to 65-day samples of fruits. The results (Table 5 and Figure 3) showed that three kinds of components accumulated with the growth time. But there were remarkable differences in their contents at different harvest time. For example, there was few nootkatone before 27 days in fruits. With the growth of fruit, the content of nootkatone entered a rapid growth phase and reached the maximum (mean value as 1.557 mg/g) at 45-day. From 45-day to 55-day, the content of nootkatone in fruits showed a little downward trend from 1.557 mg/g to 1.160 mg/g. For the three diphenylheptanes compounds, the content of every compound was very low before 40-day but at 45-day all their contents increased sharply to the maximum (mean value as 2.401 mg/g for yakuchinone A, 0.0795 mg/g for yakuchinone B and 2.465 mg/g for oxyphyllacinol). After 45-day, the contents of three diphenylheptanes compounds declined to 503 μg/g for yakuchinone A, 40 μg/g for yakuchinone B and 353 μg/g for oxyphyllacinol at 65-day. The flavones compounds exhibited another characteristics. Most of flavones achieved the maximum content before 30 harvest time. The tectochrysin had the maximum content of 128 μg/g at 20-day, the izalpinin of 33.2 μg/g at 20-day, the kaempferide of 264 μg/g at 15-day and apigenin-7,4′-dimethylether of 86.7 μg/g at 15-day. Only the content of chrysin achieved the maximum of 62.9 μg/g at 45-day. The differences of content variation between flavones with other two kinds of compounds might indicate the biosynthetic pathway of flavones was differentiated with the other two types of phytochemicals.

Bottom Line: The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min.The contents of the six bioactive compounds were reported in A. oxyphylla for the first time.The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Hainan Provincial Key Laboratory of R&D on Tropical Herbs, Hainan Medical University, Haikou 571199, China ; School of Pharmacy, Hainan Medical University, Haikou, Hainan 571199, China.

ABSTRACT

Background: The dried fruits of Alpinia oxyphylla Miq have been widely used as an herbal medicine for the treatment of diarrhea and enuresis in China. Medicinal chemistry studies revealed that the tepenes, diphenylheptanes and flavones were the main components. Therefore, these three kinds of components should be chosen as the bioactive marker compounds for the quality control of A. oxyphylla fruits. Moreover, multiple active components has been widely recognized to be a more feasible method for the quality control of herbal medicines. This study firstly provided a better method for comprehensive component analysis of A. oxyphylla fruits. Meanwhile, the different harvest time was also evaluated.

Results: The solvent-to-sample ratio was the most important factor comparing with solvent, extraction time and temperature. The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min. The optimized analytical method for ultra fast high performance liquid chromatography (UFLC) was a gradient elution using water containing 0.04‰ formic acid (A) and methanol containing 0.04‰ formic acid (B), at a flow rate of 0.3 mL/min. Using this optimized method, nine compounds were simultaneously separated and quantified by UFLC coupled with tandem electro-spray ionized mass spectrometry (MS/MS).

Conclusions: The contents of the six bioactive compounds were reported in A. oxyphylla for the first time. The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

No MeSH data available.


Related in: MedlinePlus