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Analysis of nine compounds from Alpinia oxyphylla fruit at different harvest time using UFLC-MS/MS and an extraction method optimized by orthogonal design.

Li YH, Chen F, Wang JF, Wang Y, Zhang JQ, Guo T - Chem Cent J (2013)

Bottom Line: The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min.The contents of the six bioactive compounds were reported in A. oxyphylla for the first time.The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Hainan Provincial Key Laboratory of R&D on Tropical Herbs, Hainan Medical University, Haikou 571199, China ; School of Pharmacy, Hainan Medical University, Haikou, Hainan 571199, China.

ABSTRACT

Background: The dried fruits of Alpinia oxyphylla Miq have been widely used as an herbal medicine for the treatment of diarrhea and enuresis in China. Medicinal chemistry studies revealed that the tepenes, diphenylheptanes and flavones were the main components. Therefore, these three kinds of components should be chosen as the bioactive marker compounds for the quality control of A. oxyphylla fruits. Moreover, multiple active components has been widely recognized to be a more feasible method for the quality control of herbal medicines. This study firstly provided a better method for comprehensive component analysis of A. oxyphylla fruits. Meanwhile, the different harvest time was also evaluated.

Results: The solvent-to-sample ratio was the most important factor comparing with solvent, extraction time and temperature. The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min. The optimized analytical method for ultra fast high performance liquid chromatography (UFLC) was a gradient elution using water containing 0.04‰ formic acid (A) and methanol containing 0.04‰ formic acid (B), at a flow rate of 0.3 mL/min. Using this optimized method, nine compounds were simultaneously separated and quantified by UFLC coupled with tandem electro-spray ionized mass spectrometry (MS/MS).

Conclusions: The contents of the six bioactive compounds were reported in A. oxyphylla for the first time. The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of the nine phytochemicals.
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Figure 1: Chemical structures of the nine phytochemicals.

Mentions: The dried fruits of A. oxyphylla of different harvest time (10-day, 15-day, 20-day, 25-day, 27-day, 30-day, 35-day, 40-day, 45-day, 50-day, 55-day, 60-day and 65-day) were collected from Qiongzhong county, Hainan province, China. The material was identified by vice Prof. Jian-ping Tian of Hainan Medical University. Reference standards of nootkatone, yakuchinone A, yakuchinone B, oxyphyllacinol, tectochrysin, izalpinin, chrysin, kaempferide and apigenin-7,4′-dimethylether were isolated and purified in our laboratory. On the basis of UV, NMR and MS analysis, the structures of isolated reference standards were confirmed, and their purities determined using HPLC–PDA-MS were over 98.0%. Their structures are presented in Figure 1. Methanol was HPLC-grade from Merck (Darmstadt, Germany) and deionized water was purified by a Cascada IV super purification system (Pall Corporation, NY, USA). Other reagent solutions were analytical grade (Shanghai Chemical Reagent Company, Shanghai, PR China).


Analysis of nine compounds from Alpinia oxyphylla fruit at different harvest time using UFLC-MS/MS and an extraction method optimized by orthogonal design.

Li YH, Chen F, Wang JF, Wang Y, Zhang JQ, Guo T - Chem Cent J (2013)

Chemical structures of the nine phytochemicals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750461&req=5

Figure 1: Chemical structures of the nine phytochemicals.
Mentions: The dried fruits of A. oxyphylla of different harvest time (10-day, 15-day, 20-day, 25-day, 27-day, 30-day, 35-day, 40-day, 45-day, 50-day, 55-day, 60-day and 65-day) were collected from Qiongzhong county, Hainan province, China. The material was identified by vice Prof. Jian-ping Tian of Hainan Medical University. Reference standards of nootkatone, yakuchinone A, yakuchinone B, oxyphyllacinol, tectochrysin, izalpinin, chrysin, kaempferide and apigenin-7,4′-dimethylether were isolated and purified in our laboratory. On the basis of UV, NMR and MS analysis, the structures of isolated reference standards were confirmed, and their purities determined using HPLC–PDA-MS were over 98.0%. Their structures are presented in Figure 1. Methanol was HPLC-grade from Merck (Darmstadt, Germany) and deionized water was purified by a Cascada IV super purification system (Pall Corporation, NY, USA). Other reagent solutions were analytical grade (Shanghai Chemical Reagent Company, Shanghai, PR China).

Bottom Line: The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min.The contents of the six bioactive compounds were reported in A. oxyphylla for the first time.The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Hainan Provincial Key Laboratory of R&D on Tropical Herbs, Hainan Medical University, Haikou 571199, China ; School of Pharmacy, Hainan Medical University, Haikou, Hainan 571199, China.

ABSTRACT

Background: The dried fruits of Alpinia oxyphylla Miq have been widely used as an herbal medicine for the treatment of diarrhea and enuresis in China. Medicinal chemistry studies revealed that the tepenes, diphenylheptanes and flavones were the main components. Therefore, these three kinds of components should be chosen as the bioactive marker compounds for the quality control of A. oxyphylla fruits. Moreover, multiple active components has been widely recognized to be a more feasible method for the quality control of herbal medicines. This study firstly provided a better method for comprehensive component analysis of A. oxyphylla fruits. Meanwhile, the different harvest time was also evaluated.

Results: The solvent-to-sample ratio was the most important factor comparing with solvent, extraction time and temperature. The highest yield of nine compounds was achieved with 70% ethanol-water and a solvent-to-sample ratio of 20:1 at 60°C for 30 min. The optimized analytical method for ultra fast high performance liquid chromatography (UFLC) was a gradient elution using water containing 0.04‰ formic acid (A) and methanol containing 0.04‰ formic acid (B), at a flow rate of 0.3 mL/min. Using this optimized method, nine compounds were simultaneously separated and quantified by UFLC coupled with tandem electro-spray ionized mass spectrometry (MS/MS).

Conclusions: The contents of the six bioactive compounds were reported in A. oxyphylla for the first time. The contents of nine compounds of different harvest time fruits of A. oxyphylla were assessed under the optimized extraction and UFLC-MS/MS analytical conditions, and the 45-day culture fruit had the highest content levels.

No MeSH data available.


Related in: MedlinePlus