Limits...
Effects of uric acid on endothelial dysfunction in early chronic kidney disease and its mechanisms.

Wang Y, Bao X - Eur. J. Med. Res. (2013)

Bottom Line: An increase in serum uric acid (UA) occurs during the early and middle stages of chronic kidney disease (CKD) and aggravates the deterioration of kidney function.Linear regression analysis showed that the level of serum UA had a significant positive correlation with serum endothelin-1 and the percentage of collagen I positive area, but a negative correlation with serum nitric oxide (NO) and NO/endothelin-1 ratio.In addition, the level of serum UA had significant positive correlations with serum malonaldehyde, serum C-reactive protein, serum oxidatively-modified low-density lipoprotein and serum low-density lipoprotein, but a negative correlation with serum superoxide dismutase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Nephrology, Jinshan Hospital affiliated to Fudan University, No,1508 Longhang Road, Jinshan District, Shanghai 201508, China.

ABSTRACT

Background: An increase in serum uric acid (UA) occurs during the early and middle stages of chronic kidney disease (CKD) and aggravates the deterioration of kidney function. This study aims to explore the relation between UA and endothelial dysfunction in early CKD and its mechanisms in a murine model.

Methods: The experimental animals were randomly divided into three groups (n = 10): sham-operation group (control group), right nephrectomy only group (CKD group) and right nephrectomy with oxonic potassium group (CKD with hyperuricemia group). Furthermore, we analyzed the relation between UA and endothelial dysfunction indices in early CKD as well as its mechanisms.

Results: Linear regression analysis showed that the level of serum UA had a significant positive correlation with serum endothelin-1 and the percentage of collagen I positive area, but a negative correlation with serum nitric oxide (NO) and NO/endothelin-1 ratio. In addition, the level of serum UA had significant positive correlations with serum malonaldehyde, serum C-reactive protein, serum oxidatively-modified low-density lipoprotein and serum low-density lipoprotein, but a negative correlation with serum superoxide dismutase.

Conclusions: Endothelial dysfunction in the CKD group was significant and had a positive correlation with the level of serum UA. Endothelial dysfunction in early CKD with hyperuricemia is perhaps related to oxidative stress, micro-inflammation and lipid oxidation.

Show MeSH

Related in: MedlinePlus

Pathological pictures of rat kidneys from groups A, B, and C. (A) The normal renal glomerulus of group A (×400). (B) No obvious pathological change in renal tubules of group A (×400). (C, E) Mild glomerular mesangial proliferation in renal glomerulus of groups B and C (×400). (D, F) There were no obvious pathological changes in renal tubules in groups B and C (×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750429&req=5

Figure 1: Pathological pictures of rat kidneys from groups A, B, and C. (A) The normal renal glomerulus of group A (×400). (B) No obvious pathological change in renal tubules of group A (×400). (C, E) Mild glomerular mesangial proliferation in renal glomerulus of groups B and C (×400). (D, F) There were no obvious pathological changes in renal tubules in groups B and C (×400).

Mentions: Periodic acid-Schiff staining of kidney tissues from each group showed mild glomerular mesangial proliferation in groups B and C, compared to group A (Figure 1A,C,E). No obvious pathological change in renal tubule and renal interstitium was visible in the three groups. Meanwhile, there was no renal tubular epithelial cells necrosis, no inflammatory cells in the interstitium, and no small vessel lesions in groups B and C (Figure 1D,F). In the experimental group, there was no urate crystal deposition. Compared with groups A and B, the experimental group had a significantly higher level of UA. However, there was no obvious difference in Scr among the three groups (Table 1), which was in accordance with the characteristics of early CKD. The above results indicate the establishment of an early CKD animal model with hyperuricemia.


Effects of uric acid on endothelial dysfunction in early chronic kidney disease and its mechanisms.

Wang Y, Bao X - Eur. J. Med. Res. (2013)

Pathological pictures of rat kidneys from groups A, B, and C. (A) The normal renal glomerulus of group A (×400). (B) No obvious pathological change in renal tubules of group A (×400). (C, E) Mild glomerular mesangial proliferation in renal glomerulus of groups B and C (×400). (D, F) There were no obvious pathological changes in renal tubules in groups B and C (×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750429&req=5

Figure 1: Pathological pictures of rat kidneys from groups A, B, and C. (A) The normal renal glomerulus of group A (×400). (B) No obvious pathological change in renal tubules of group A (×400). (C, E) Mild glomerular mesangial proliferation in renal glomerulus of groups B and C (×400). (D, F) There were no obvious pathological changes in renal tubules in groups B and C (×400).
Mentions: Periodic acid-Schiff staining of kidney tissues from each group showed mild glomerular mesangial proliferation in groups B and C, compared to group A (Figure 1A,C,E). No obvious pathological change in renal tubule and renal interstitium was visible in the three groups. Meanwhile, there was no renal tubular epithelial cells necrosis, no inflammatory cells in the interstitium, and no small vessel lesions in groups B and C (Figure 1D,F). In the experimental group, there was no urate crystal deposition. Compared with groups A and B, the experimental group had a significantly higher level of UA. However, there was no obvious difference in Scr among the three groups (Table 1), which was in accordance with the characteristics of early CKD. The above results indicate the establishment of an early CKD animal model with hyperuricemia.

Bottom Line: An increase in serum uric acid (UA) occurs during the early and middle stages of chronic kidney disease (CKD) and aggravates the deterioration of kidney function.Linear regression analysis showed that the level of serum UA had a significant positive correlation with serum endothelin-1 and the percentage of collagen I positive area, but a negative correlation with serum nitric oxide (NO) and NO/endothelin-1 ratio.In addition, the level of serum UA had significant positive correlations with serum malonaldehyde, serum C-reactive protein, serum oxidatively-modified low-density lipoprotein and serum low-density lipoprotein, but a negative correlation with serum superoxide dismutase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Nephrology, Jinshan Hospital affiliated to Fudan University, No,1508 Longhang Road, Jinshan District, Shanghai 201508, China.

ABSTRACT

Background: An increase in serum uric acid (UA) occurs during the early and middle stages of chronic kidney disease (CKD) and aggravates the deterioration of kidney function. This study aims to explore the relation between UA and endothelial dysfunction in early CKD and its mechanisms in a murine model.

Methods: The experimental animals were randomly divided into three groups (n = 10): sham-operation group (control group), right nephrectomy only group (CKD group) and right nephrectomy with oxonic potassium group (CKD with hyperuricemia group). Furthermore, we analyzed the relation between UA and endothelial dysfunction indices in early CKD as well as its mechanisms.

Results: Linear regression analysis showed that the level of serum UA had a significant positive correlation with serum endothelin-1 and the percentage of collagen I positive area, but a negative correlation with serum nitric oxide (NO) and NO/endothelin-1 ratio. In addition, the level of serum UA had significant positive correlations with serum malonaldehyde, serum C-reactive protein, serum oxidatively-modified low-density lipoprotein and serum low-density lipoprotein, but a negative correlation with serum superoxide dismutase.

Conclusions: Endothelial dysfunction in the CKD group was significant and had a positive correlation with the level of serum UA. Endothelial dysfunction in early CKD with hyperuricemia is perhaps related to oxidative stress, micro-inflammation and lipid oxidation.

Show MeSH
Related in: MedlinePlus