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Enhancing performance of a miniaturized surface plasmon resonance sensor in the reflectance detection mode using a waveguide-coupled bimetallic chip.

Lee YK, Jang DH, Lee KS, Kim WM, Sohn YS - Nanoscale Res Lett (2013)

Bottom Line: In the intensity measurement mode, the sensitivity could be improved by reducing the full width at half maximum (FWHM) of the reflectance curve.Enhancement of the sensitivity in the intensity detection mode was achieved using the WcBiM chip compared with the Au chip.Therefore, sufficient sensitivity for the detection of a disease-related biomarker is attainable with the WcBiM chip in the intensity measurement mode using a miniaturized SPR sensor.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Catholic University of Daegu, 13-13 Hayang-ro, Hayang-eup, Gyeongsan-si, Gyeongbuk 712-702, Republic of Korea. sohnys@cu.ac.kr.

ABSTRACT
The characteristics of a waveguide-coupled bimetallic (WcBiM) chip in a miniaturized surface plasmon resonance (SPR) sensor and its detection capability for a low molecular weight biomolecule were investigated. The configuration of the WcBiM chip was gold (Au)/waveguide (ZnS-SiO2)/silver (Ag). In the intensity measurement mode, the sensitivity could be improved by reducing the full width at half maximum (FWHM) of the reflectance curve. The FWHM of the WcBiM chip is narrower than that of the Au chip, which suggests that the slope of the reflectance curve for the WcBiM chip is steeper. In order to generate enhanced resolution, the reflectance should be monitored at the specific angle where the slope is the steepest in the reflectance curve. For the detection of biotin that is a low molecular weight biomolecule, streptavidin was formed on the SPR sensor chip surface. The response of the SPR to biotin at various concentrations was then acquired. The sensitivities of the WcBiM chip and the Au chip were 0.0052%/(ng/ml) and 0.0021%/(ng/ml), respectively. The limit of detection of the biotin concentration for both the WcBiM and Au chips was calculated. The values were 2.87 ng/ml for the WcBiM chip and 16.63 ng/ml for the Au chip. Enhancement of the sensitivity in the intensity detection mode was achieved using the WcBiM chip compared with the Au chip. Therefore, sufficient sensitivity for the detection of a disease-related biomarker is attainable with the WcBiM chip in the intensity measurement mode using a miniaturized SPR sensor.

No MeSH data available.


Related in: MedlinePlus

Linearity in calibration curves between SPR response and biotin concentration ranging from 50 to 200 ng/ml.
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Figure 7: Linearity in calibration curves between SPR response and biotin concentration ranging from 50 to 200 ng/ml.

Mentions: To confirm these results, Figure 7 presents the reflectance change as a function of the concentration of biotin. The sensitivity can be defined as the derivative of the SPR response with respect to the analyte concentration and is an extract from the linear part of the calibration curve for each of the investigated analytes. Thus, the sensitivity can be obtained by the slope (ΔReflectance (%)/ΔConcentration (ng/ml)) of their respective linear relations. The slopes for the SPR responses of biotin in the WcBiM chip and the Au chip were 0.0052%/(ng/ml) and 0.0021%/(ng/ml), respectively. This shows that the sensitivity of the WcBiM chip was twice that of the Au chip. Thus, the experimental results showed that the WcBiM chip enhances sensitivity in the reflectance measurement mode.


Enhancing performance of a miniaturized surface plasmon resonance sensor in the reflectance detection mode using a waveguide-coupled bimetallic chip.

Lee YK, Jang DH, Lee KS, Kim WM, Sohn YS - Nanoscale Res Lett (2013)

Linearity in calibration curves between SPR response and biotin concentration ranging from 50 to 200 ng/ml.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750426&req=5

Figure 7: Linearity in calibration curves between SPR response and biotin concentration ranging from 50 to 200 ng/ml.
Mentions: To confirm these results, Figure 7 presents the reflectance change as a function of the concentration of biotin. The sensitivity can be defined as the derivative of the SPR response with respect to the analyte concentration and is an extract from the linear part of the calibration curve for each of the investigated analytes. Thus, the sensitivity can be obtained by the slope (ΔReflectance (%)/ΔConcentration (ng/ml)) of their respective linear relations. The slopes for the SPR responses of biotin in the WcBiM chip and the Au chip were 0.0052%/(ng/ml) and 0.0021%/(ng/ml), respectively. This shows that the sensitivity of the WcBiM chip was twice that of the Au chip. Thus, the experimental results showed that the WcBiM chip enhances sensitivity in the reflectance measurement mode.

Bottom Line: In the intensity measurement mode, the sensitivity could be improved by reducing the full width at half maximum (FWHM) of the reflectance curve.Enhancement of the sensitivity in the intensity detection mode was achieved using the WcBiM chip compared with the Au chip.Therefore, sufficient sensitivity for the detection of a disease-related biomarker is attainable with the WcBiM chip in the intensity measurement mode using a miniaturized SPR sensor.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Catholic University of Daegu, 13-13 Hayang-ro, Hayang-eup, Gyeongsan-si, Gyeongbuk 712-702, Republic of Korea. sohnys@cu.ac.kr.

ABSTRACT
The characteristics of a waveguide-coupled bimetallic (WcBiM) chip in a miniaturized surface plasmon resonance (SPR) sensor and its detection capability for a low molecular weight biomolecule were investigated. The configuration of the WcBiM chip was gold (Au)/waveguide (ZnS-SiO2)/silver (Ag). In the intensity measurement mode, the sensitivity could be improved by reducing the full width at half maximum (FWHM) of the reflectance curve. The FWHM of the WcBiM chip is narrower than that of the Au chip, which suggests that the slope of the reflectance curve for the WcBiM chip is steeper. In order to generate enhanced resolution, the reflectance should be monitored at the specific angle where the slope is the steepest in the reflectance curve. For the detection of biotin that is a low molecular weight biomolecule, streptavidin was formed on the SPR sensor chip surface. The response of the SPR to biotin at various concentrations was then acquired. The sensitivities of the WcBiM chip and the Au chip were 0.0052%/(ng/ml) and 0.0021%/(ng/ml), respectively. The limit of detection of the biotin concentration for both the WcBiM and Au chips was calculated. The values were 2.87 ng/ml for the WcBiM chip and 16.63 ng/ml for the Au chip. Enhancement of the sensitivity in the intensity detection mode was achieved using the WcBiM chip compared with the Au chip. Therefore, sufficient sensitivity for the detection of a disease-related biomarker is attainable with the WcBiM chip in the intensity measurement mode using a miniaturized SPR sensor.

No MeSH data available.


Related in: MedlinePlus