Limits...
Five markers useful for the distinction of canine mammary malignancy.

Pawłowski KM, Maciejewski H, Majchrzak K, Dolka I, Mol JA, Motyl T, Król M - BMC Vet. Res. (2013)

Bottom Line: There are significant differences in survival between cases with different tumor grades.The expression of this gene set (sehrl, zfp37, mipep, relaxin, and magi3) differs significantly in the most malignant tumors at mRNA level as well as at protein level.These genes are also interesting as targets for further investigations and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - WULS, Nowoursynowska 159, 02-776, Warsaw, Poland.

ABSTRACT

Background: Spontaneous canine mammary tumors constitute a serious clinical problem. There are significant differences in survival between cases with different tumor grades. Unfortunately, the distinction between various grades is not clear. A major problem in evaluating canine mammary cancer is identifying those, that are "truly" malignant. That is why the aim of our study was to find the new markers of canine malignancy, which could help to diagnose the most malignant tumors.

Results: Analysis of gene expression profiles of canine mammary carcinoma of various grade of malignancy followed by the boosted tree analysis distinguished a `gene set`. The expression of this gene set (sehrl, zfp37, mipep, relaxin, and magi3) differs significantly in the most malignant tumors at mRNA level as well as at protein level. Despite this `gene set` is very interesting as an additional tool to estimate canine mammary malignancy, it should be validated using higher number of samples.

Conclusions: The proposed gene set can constitute a `malignancy marker` that could help to distinguish the most malignant canine mammary carcinomas. These genes are also interesting as targets for further investigations and therapy. So far, only two of them were linked with the cancer development.

Show MeSH

Related in: MedlinePlus

Expression of `diagnostic gene set` assessed using real-time qPCR. Expression of `diagnostic gene set` in canine mammary carcinoma of various grade of malignancy. The changes in gene expression which differed significantly (p < 0.05) markered as *, the changes in gene expression which differed highly significant (p < 0.01 and p < 0.001) markered as ** and ***, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3750412&req=5

Figure 2: Expression of `diagnostic gene set` assessed using real-time qPCR. Expression of `diagnostic gene set` in canine mammary carcinoma of various grade of malignancy. The changes in gene expression which differed significantly (p < 0.05) markered as *, the changes in gene expression which differed highly significant (p < 0.01 and p < 0.001) markered as ** and ***, respectively.

Mentions: Real-Time qPCR analysis confirmed similar trends in expression of the examined five genes (Figure 2). To check the expression of the selected `gene set` in mammary tumors in general, all the 18 tumors were under analysis using microarrays [1]. These tumors were classified by pathologists as grade I, grade II and grade III (six of each group).


Five markers useful for the distinction of canine mammary malignancy.

Pawłowski KM, Maciejewski H, Majchrzak K, Dolka I, Mol JA, Motyl T, Król M - BMC Vet. Res. (2013)

Expression of `diagnostic gene set` assessed using real-time qPCR. Expression of `diagnostic gene set` in canine mammary carcinoma of various grade of malignancy. The changes in gene expression which differed significantly (p < 0.05) markered as *, the changes in gene expression which differed highly significant (p < 0.01 and p < 0.001) markered as ** and ***, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750412&req=5

Figure 2: Expression of `diagnostic gene set` assessed using real-time qPCR. Expression of `diagnostic gene set` in canine mammary carcinoma of various grade of malignancy. The changes in gene expression which differed significantly (p < 0.05) markered as *, the changes in gene expression which differed highly significant (p < 0.01 and p < 0.001) markered as ** and ***, respectively.
Mentions: Real-Time qPCR analysis confirmed similar trends in expression of the examined five genes (Figure 2). To check the expression of the selected `gene set` in mammary tumors in general, all the 18 tumors were under analysis using microarrays [1]. These tumors were classified by pathologists as grade I, grade II and grade III (six of each group).

Bottom Line: There are significant differences in survival between cases with different tumor grades.The expression of this gene set (sehrl, zfp37, mipep, relaxin, and magi3) differs significantly in the most malignant tumors at mRNA level as well as at protein level.These genes are also interesting as targets for further investigations and therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - WULS, Nowoursynowska 159, 02-776, Warsaw, Poland.

ABSTRACT

Background: Spontaneous canine mammary tumors constitute a serious clinical problem. There are significant differences in survival between cases with different tumor grades. Unfortunately, the distinction between various grades is not clear. A major problem in evaluating canine mammary cancer is identifying those, that are "truly" malignant. That is why the aim of our study was to find the new markers of canine malignancy, which could help to diagnose the most malignant tumors.

Results: Analysis of gene expression profiles of canine mammary carcinoma of various grade of malignancy followed by the boosted tree analysis distinguished a `gene set`. The expression of this gene set (sehrl, zfp37, mipep, relaxin, and magi3) differs significantly in the most malignant tumors at mRNA level as well as at protein level. Despite this `gene set` is very interesting as an additional tool to estimate canine mammary malignancy, it should be validated using higher number of samples.

Conclusions: The proposed gene set can constitute a `malignancy marker` that could help to distinguish the most malignant canine mammary carcinomas. These genes are also interesting as targets for further investigations and therapy. So far, only two of them were linked with the cancer development.

Show MeSH
Related in: MedlinePlus